Nucleotide sequences of the attachment sites of bacteriophage Mu DNA

Kahmann, Regine; Kamp, Dietmar

doi:10.1038/280247a0

Cited by 87 publications

(50 citation statements)

References 20 publications

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“…It can catalyze the full spectrum of processes normally attributed to bacterial transposons, such as chromosomal rearrangements (3) and precise and imprecise excision (4,5). Moreover, Mu has also been found to induce five base pair duplications at its insertion site (6,7) as has been previously reported for IS2 (8), Tn3 (9), a transposable sequence carried on pSC101 (10) and yb (11). Recently, a comparison of the nucleotide sequences of Mu and a number of transposable elements has also revealed a common heptanucleotide sequence near their termini (11).…”

mentioning

confidence: 77%

Association of Mu-containing plasmids with the Escherichia coli chromosome upon prophage induction

Chaconas¹,

Harshey²,

Bukhari³

1980

Proc. Natl. Acad. Sci. U.S.A.

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confidence: 77%

Association of Mu-containing plasmids with the Escherichia coli chromosome upon prophage induction

Chaconas¹,

Harshey²,

Bukhari³

1980

Proc. Natl. Acad. Sci. U.S.A.

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“…toward the control region of lacZ when it is not being transcribed (42). Phage genome-host DNA junction sequence determination revealed some preference (43) at the 5-bp target site duplication (44,45). An analysis of junction sequences of mini-Mu insertion sites further revealed a similar consensus sequence of duplication (5Ј-NY(G/C)RN-3Ј) from in vivo and in vitro transposition (46).…”

mentioning

confidence: 99%

DNA Transposition of Bacteriophage Mu

Haapa-Paananen¹,

Rita²,

Savilahti³

2002

Journal of Biological Chemistry

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The Mu transpositional DNA recombination machinery selects target sites by assembling a protein-DNA complex that interacts with the target DNA and reacts whenever it locates a favorable sequence composition. Splicing of a transposon into the target generates a 5-bp duplication that reflects the original target site. Preferential usage of different target pentamers was examined with a minimal Mu in vitro system and quantitatively compiled consensus sequences for the most preferred and the least preferred sites were generated. When analyzed as base steps, preferences toward certain steps along the 5-bp target site were detected. We further show that insertion sites can be predicted on the basis of additively calculated base step values. Also surrounding sequences influence the preference of a given pentamer; a symmetrical structural component was revealed, suggesting potential hinges at and around the target site.

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“…We therefore examined whether evidence for linkage between a cspA homologue and the Mudlux element in the MPG300 derivative MPG361 could be obtained by PCR. Primers were designed which corresponded to the first eighteen bases of the coding region (from the ATG) of the sense strand of the E. coli cspA gene (Goldstein et al, 1990), and to a twenty base region which corresponded to nucleotide residues 83-64 from the terminus of the P-segment of phage Mu (Kahmann & Kamp, 1979). Amplification of chromosomal DNA from MPG361 resulted in a fragment of approximately 150 base pairs which was subsequently blunt-ended with Klenow polymerase and inserted into the SmaI site of pBluescript KS (Short et al, 1988), forming plasmid pKPF1.…”

Section: Resultsmentioning

confidence: 99%

“…The threshold temperature for light expression correlates with cspB mRNA levels and involves differential mRNA stability Analysis of the sequence of the insert in pKPFl revealed that an additional T residue lies between residues G,, and T,, of the published sequence of the /I-end of the phage Mu sequence (Kahmann & Kamp, 1979) and that a continuous ORF encoding a polypeptide of 71 amino acids had formed between the cspB gene and the Mudlux element. It therefore remained possible that light production from MPG361 at or below 22 "C (Fig.…”

Section: Bioluminescence From Mpg361 Occurs Below a Threshold Temperamentioning

confidence: 99%

Expression of the cold-shock gene cspB in Salmonella typhimurium occurs below a threshold temperature

Craig

Boyle²,

Francis³

et al. 1998

Microbiology

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Previous studies have shown that several bacterial species exhibit a multigenic response following temperature downshift (cold shock). Evidence for such a response in Salmonella typhimurium is reported, based on the isolation of a range of low-induction-temperature gene fusions containing Mudlux insertions. The fusions exhibited different levels of basal light at 30 "C, and were induced at different rates and to different degrees over several hours following a reduction in temperature to 10 OC. Of the Mudlux gene fusions isolated, one was found which produced essentially no light when grown at 30 "C but exhibited rapid and high-level induction when the temperature was reduced to I 0 OC. The target of this gene fusion (which was named CSlpB) was shown to lie adjacent to the umuDC operon and to encode a homologue of the major cold-shock protein of Escherichia coli, CspA. Luminescence studies revealed that substantial light production occurred from the cspB: : Mudlux fusion at or below 22 "C but not at higher temperatures, even following a temperature drop from 30 OC. Moreover, crpB mRNA levels were found to mimic this pattern of luminescence, suggesting that cspB expression occurs below a defined temperature threshold. The cspB mRNA was also found to be very stable at 10 "C but to become highly unstable when the temperature was raised towards the threshold temperature, even in the presence of rifampicin. Existing cellular RNases therefore appear to mediate the decay of crpB mRNA at high temperatures, but are incapable of this at low temperatures.

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Nucleotide sequences of the attachment sites of bacteriophage Mu DNA

Cited by 87 publications

References 20 publications

Association of Mu-containing plasmids with the Escherichia coli chromosome upon prophage induction

Association of Mu-containing plasmids with the Escherichia coli chromosome upon prophage induction

DNA Transposition of Bacteriophage Mu

Expression of the cold-shock gene cspB in Salmonella typhimurium occurs below a threshold temperature

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