Nucleoside by-product dietary supplementation influences blood chemistry, immune response, oxidative stress resistance and intestinal morphology of juvenile amberjack,Seriola dumerili

Abstract

We explored the influence of nucleoside by-products (NBP) on blood chemistry, immune response, oxidative stress and intestinal morphology of Seriola dumerili. Four experimental diets were formulated where diets 1-3 are semipurified and supplemented with liquid NBP at concentration of 0 (D1, negative control), 30 (D2) and 90 (D3) g/kg. Diet 4 (D4) is a fishmeal-based positive control diet. Each diet was randomly allocated to triplicate groups of fish for 50 days. The results revealed a significant influence of NBP supplementations on total cholesterol, blood urea nitrogen and triglyceride level. Lysozyme activity (LA), total serum protein (TSP) and peroxidase activity (PA) also increased with NBP supplementation as compared to the negative control.TSP, PA and LA were numerically higher in diet groups D2 and D4, respectively.Anterior enterocyte height (hE) was higher in diet group D4, while NBP-supplemented groups showed intermediate values with D1 group and negative control group showed significantly lowest value. Supplementation of NBP also increased anterior fold height (hF) and microvillus height and posterior hF, hE and microvillus height compared with D1. Finally, we concluded that NBP can be used as a cheaper nucleotide/nucleoside source for amberjack. Supplementation of more than 30 g/kg in diets improved blood chemistry, non-specific immunity, oxidative stress resistance and intestinal morphology of amberjack. K E Y W O R D Samberjack, blood chemistry, inosine, intestinal morphology, nucleoside by-product, oxidative stress 1

Dietary nucleotide-rich yeast supplementation improves growth, innate immunity and intestinal morphology of Pacific white shrimp (Litopenaeus vannamei)

“…In contrast, the results of present study indicated that dietary NT-rich yeast supplementation did not significantly affect T-NOS and T-SOD activities for shrimp. Thus, although there are considerable gaps in existing knowledge about the effects of the NT diets on shrimp's immune responses, it was clear that yeast NT exerted a beneficial effects on immunity in our study, which agrees with previous findings in amberjack (Hossain et al, 2017), common carp (Sakai et al, 2001), olive flounder (Paralichthys olivaceus) (Song, Lim, & Lee, 2012), rainbow trout (Yousefi et al, 2016), red drum (Sciaenops ocellatus) (Cheng et al, 2011), sea bream (Pagrus major) and tilapia (Xu et al, 2015). However, high levels of dietary NT could cause high serum uric acid, toxicological effects and interference with the metabolism of other nutrients in most monogastric animals (Rumsey, Winfree, & Hughes, 1992).…”

“…Serum biochemical parameters in the current experiment were within the normal range for L. vannamei (Cao et al., ; Hu et al., ). TP concentration in serum is the most essential compound and it is important for sustaining a healthy immune system (Hossain et al., ). In the present study, increased serum TP in the NT‐rich yeast‐supplemented diets suggested that dietary NT‐rich yeast may act as an immunostimulant for shrimp.…”

“…Recently, Hossain et al. () reported that dietary NT supplementation increased fold height, enterocyte height and microvillus height in anterior and posterior intestine of juvenile amberjack ( Seriola dumerili ). In the present study, shrimp fed the diet containing 30 g/kg NT‐rich yeast had higher intestinal fold height, fold width and enterocyte height than those fed the control diet, and shrimp fed the 50 g/kg NT‐rich yeast diet had the highest microvillus height among all treatments.…”

Spawning Induction of First-Generation (F1) Greater Amberjack Seriola dumerili in the Canary Islands, Spain Using GnRHa Delivery Systems

“…Analysis of hematological and biochemical parameters in the blood is a valuable tool that can be used as an effective index to monitor fish health and pathological changes. Both hematological and biochemical parameters obtained in the present experiment were within the normal range for greater amberjack, compared to previous studies [80][81][82]. Most blood parameters studied remained constant along the study and only erythrocytes, protein, cholesterol and amylase were lower at the end of the spawning season.…”

Increased parasite resistance of greater amberjack (Seriola dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues

“…Little information is available about the immune system of greater amberjack [10,35] and few studies have investigated the use of immunostimulants with this species [36,37,38], with none using MOS or concentrated MOS (cMOS) inclusion. For this reason, the objective of the present work was to determine the effect of MOS and cMOS (Bio-Mos® and Actigen®) and their combination on greater amberjack juveniles, focusing on immune parameters, protective effects against a N. girellae and any impact on growth/feed efficiency.…”