Nucleoporin Nup98 participates in flowering regulation in a CONSTANS-independent mode

Jiang, Shanshan; Xiao, Long; Huang, Penghui; Cheng, Zhiyuan; Chen, Fulu; Miao, Yuchen; Fu, Yong-Fu; Chen, Qingshan; Zhang, Xiaomei

doi:10.1007/s00299-019-02442-w

Cited by 15 publications

(36 citation statements)

References 54 publications

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“…The results indicated that the mechanism of senescence regulation in the nup98 double mutant was much complicated. Many genes showed circadian expression pattern, consistent with our previous report that Nup98 participated in regulation circadian clock [12].…”

Section: Nup98 Gene May Be Involved At Multiple Pathways Of Senescencsupporting

confidence: 91%

“…Recently in Arabidopsis, the interaction between Nup98 and Nup88/MOS7 is required for plant immunity against the necrotrophic fungal pathogen Botrytis cinerea and mitogen-activated protein kinase signalling [11]. We found Nup98 contributes to flowering regulation in Arabidopsis [12].…”

Section: Introductionmentioning

confidence: 77%

“…We just reported that the nup98a1 nup98b1 ft-10 triple mutant displayed the late flowering character as the ft-10 mutant but maintained early senescence phenotypes of the nup98a1 nup8b1 double mutant [12], suggesting that Nup98a and Nup98b were involved in regulation of both flowering and senescence, two developmental processes individually regulated by Nup98a and Nup98b.…”

Section: Discussionmentioning

confidence: 94%

“…Many genes studied in this study showed time point-dependent changes. We recently found that the expression level of ELF3 and other clock genes significantly reduced in the nup98a nup98b double mutant [12].…”

Section: Discussionmentioning

confidence: 98%

“…These genes encoded enzymes for the degradation of starch not only in the early steps of starch degradation in chloroplasts [18], such as GWD1 (SEX1), β-BAM1, BAM3, BAM5, BAM6, BAM7, BAM8, SEX4, and LSF1, but also in the later steps of starch degradation, such as limit-dextrinase (LDA), α-amylase (AMY1 and AMY2), disproportioning enzyme (DPE1 and DPE2), α-glucan phosphorylase (PHS1 and PHS2). Time-dependent low-expression of these genes suggested they were in the control of circadian clock, since Nup98 is involved in circadian regulation in Arabidopsis [12]. These genes function at different steps [18].…”

Section: Starch Metabolism Is Impaired In the Nup98a Nup98b Double Mumentioning

confidence: 99%

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Two Nucleoporin 98 homologous genes jointly participate in the regulation of starch degradation to enhance growth in Arabidopsis

Xiao

Jiang

Huang

et al. 2020

Preprint

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Background: Starch is synthesized during the day for temporary storage in leaves and then degraded during the subsequent night to support plant growth and development. Impairment of starch degradation leads to stunted growth, even senescence and death. The nuclear pore complex is involved in many cellular processes, but its relationship with starch degradation is unclear until now. We previously identified two Nucleoporin98 ( Nup98 ) genes ( Nup98a and Nup98b ) redundantly regulated flowering through CONSTANS ( CO ) independent pathway. in Arabidopsis thaliana. The nup98a nup98b double mutant also showed severe senescence phenotypes. Results: We found that Nucleoporin 98 ( Nup98 ) participated in the regulation of sugar metabolism in leaves and in turn is involved in senescence regulation in Arabidopsis . We show that Nup98a and Nup98b redundantly function in the different steps of starch degradation, the nup98a nup98b double mutant accumulates more starch than wild type and has a severe early senescence phenotype compared to wild type. The expression of marker genes related to starch degradation was impaired in the nup98a nup98b double mutant, and indicator genes of carbon starvation and senescence expressed earlier in the nup98a nup98b double mutant than that in wild type plants, suggesting abnormality of energy metabolism was the cause of senescence of the nup98a nup98b double mutant. Addition of sucrose to the growth medium can rescue early senescence phenotype of the nup98a nup98b mutant. Conclusions: Our results provided a line of evidence on a novel role of the nuclear pore complex in energy metabolism related to growth and development, whereas Nup98 functioned in starch degradation conferring growth regulation in Arabidopsis .

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Section: Nup98 Gene May Be Involved At Multiple Pathways Of Senescencsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 98%

Section: Starch Metabolism Is Impaired In the Nup98a Nup98b Double Mumentioning

confidence: 99%

See 3 more Smart Citations

Two Nucleoporin 98 homologous genes jointly participate in the regulation of starch degradation to enhance growth in Arabidopsis

Xiao

Jiang

Huang

et al. 2020

Preprint

Self Cite

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Structural and functional insight into the nuclear pore complex and nuclear transport receptors in plant stress signaling

2020

Current Opinion in Plant Biology

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Nucleoporin 160 Regulates Flowering through Anchoring HOS1 for Destabilizing CO in Arabidopsis

Liu

Teo

et al. 2020

Plant Communications

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Nuclear pore complexes (NPCs), which comprise multiple copies of nucleoporins (Nups), are large protein assemblies embedded in the nuclear envelope connecting the nucleus and cytoplasm. Although it has been known that Nups affect flowering in Arabidopsis , the underlying mechanisms are poorly understood. Here, we show that loss of function of Nucleoporin 160 ( Nup160 ) leads to increased abundance of CONSTANS (CO) protein and the resulting upregulation of FLOWERING LOCUS T ( FT ) specifically in the morning. We demonstrate that Nup160 regulates CO protein stability through affecting NPC localization of an E3-ubiquitin ligase, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 (HOS1), which destabilizes CO protein in the morning period. Taken together, these results provide a mechanistic understanding of Nup function in the transition from vegetative to reproductive growth, suggesting that deposition of HOS1 at NPCs by Nup160 is essential for preventing precocious flowering in response to photoperiod in Arabidopsis .

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Nucleoporin Nup98 participates in flowering regulation in a CONSTANS-independent mode

Cited by 15 publications

References 54 publications

Two Nucleoporin 98 homologous genes jointly participate in the regulation of starch degradation to enhance growth in Arabidopsis

Two Nucleoporin 98 homologous genes jointly participate in the regulation of starch degradation to enhance growth in Arabidopsis

Structural and functional insight into the nuclear pore complex and nuclear transport receptors in plant stress signaling

Nucleoporin 160 Regulates Flowering through Anchoring HOS1 for Destabilizing CO in Arabidopsis

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