Nucleolar organizer regions in small cell tumours of childhood

Egan, Mark; Raafat, F.; Crocker, J; Smith, Kristy

doi:10.1002/path.1711530312

Cited by 77 publications

(11 citation statements)

References 19 publications

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“…One study by Egan et al 1987 studied AgNOR counts in 20 RMS, 20 neuroblastoma, and 10 Ewings sarcoma and found that mean AgNOR counts tended to be characteristic of each particular histology with RMS having the least counts followed by Ewings Sarcoma and neuroblastoma the most. A study of 194 adult soft tissue sarcomas revealed that AgNOR count was an independent prognostic factor in a multivariate analysis with mitotic count, extent of necrosis, cellularity, cellular pleomorphism, extent of myxoid change, sclerosis, nonspecific histological diagnosis, and mast cell counts (Tomita et al, 1993).…”

Section: Discussionmentioning

confidence: 96%

Nascent pre-rRNA overexpression correlates with an adverse prognosis in alveolar rhabdomyosarcoma

Williamson

Fang

et al. 2006

Genes Chromosom. Cancer

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Comparative expressed sequence hybridization (CESH) is an expression profiling technique which identifies chromosomal regions corresponding to differential gene expression. Here, we observe that various tumor samples including rhabdomyosarcoma show very prominent staining on the short arms of the acrocentric chromosomes suggesting an increase in expression of ribosomal RNA synthesized from the repetitive rDNA of the nucleolar organizer regions located on these chromosomes. Survival analysis showed a correlation with overexpression from this region and a poor prognosis in rhabdomyosarcoma. This phenomenon was studied in an extended set of rhabdomyosarcoma tumor samples using quantitative real-time reverse transcriptase-PCR to quantify levels of pre-rRNA (precursor ribosomal RNA). It was demonstrated first that the strong CESH signals did correspond to a marked increase in pre-rRNA expression and second that high pre-rRNA expression correlated with an adverse prognosis in alveolar subtype rhabdomyosarcoma. In addition, we demonstrate that pre-rRNA expression is significantly correlated with tumor stage. We conclude that measuring expression of pre-rRNA by real-time PCR is a useful prognostic marker in alveolar rhabdomyosarcoma. Furthermore, given that we have observed similar rDNA staining in all cancer types that we have studied by CESH, we propose that pre-rRNA overexpression is a general phenomenon in cancer and that our real-time PCR assay may be applicable as a prognostic marker in other tumor types.

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Section: Discussionmentioning

confidence: 96%

Nascent pre-rRNA overexpression correlates with an adverse prognosis in alveolar rhabdomyosarcoma

Williamson

Fang

et al. 2006

Genes Chromosom. Cancer

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“…Fibrosarcoma can be differentiated from fibrous proliferations (Egan et al, 1988a) and neuroblastoma can be differentiated from other small round cell tumours on the basis of AgNOR counts (Egan et al, 1987). AgNOR numbers are strongly correlated with histopathological prognostic indices of neuroblastoma and are an independent prognostic indicator in that condition (Egan et al, 1988b).…”

Section: Endocrine Tumoursmentioning

confidence: 99%

Nucleolar organiser regions in pathology

Egan

Crocker²

1992

Br J Cancer

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“…[10][11][12][13] AgNOR analysis has been shown to be useful for diagnosis of malignant tumors of different cell types. 14,[15][16][17][18] To date only two studies have demonstrated the positive diagnostic value of AgNOR analysis in nasopharyngeal carcinoma (NPC). Their conclusions were based on optical counting of the AgNOR numbers.…”

mentioning

confidence: 99%