Nucleolar fine structure and RNA synthesis in bovine oocytes from antral follicles

Crozet, Nicole; Kaňka, Jiří; Motlı́k, Jan; Fulka, Josef

doi:10.1002/mrd.1120140108

Cited by 154 publications

(147 citation statements)

References 17 publications

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“…They displayed homogeneous contents without any granules or individual fibrils. The &dquo;nucleoli&dquo; were composed of densely packed fibrils (Crozet et al, 1986). In oocytes of antral follicles, the &dquo;nucleoli&dquo; might be composed of acid proteins (Antoine et al, 1988) or, on the contrary, be rich in basic proteins (Takeuchi, 1986).…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Rozinek¹,

Muller²,

Courtens³

1989

Reprod. Nutr. Dévelop.

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Summary ― In this preliminary work, using pig embryos ultrastructural immunocytochemistry with polyclonal antibodies against purified histones was used to demonstrate both their localization and the time of their appearance in pronuclei, from 15 h after ovulation (pronuclear stage) to 48 h postinsemination (4-cell stage).In pronuclei, the histones H2B, H3, and H4 were located in the heterochromatin as soon as it appeared. Usually, one of the pronuclei seemed to be more heavily labelled. The chromatin facing the zone of pronuclear contact formed a bowl-shaped region in each pronucleus heavily labelled for these histones. The so-called pseudo-nucleoli were present in both pronuclei and contained H2B.In 4-cell stages, the labelling intensities of heterochromatin for H2B, H3 and H4 were equal in all the nuclei. H2B was still evident in the pseudo-nucleoli, but in a lower quantity than before. The condensed chromatin located either under the nuclear envelope or surrounding the pseudo-nucleoli was heavily labelled for H2B, H3 and H4.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Rozinek¹,

Muller²,

Courtens³

1989

Reprod. Nutr. Dévelop.

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“…Competence is acquired during oocyte growth, when the synthesis and storage of proteins and RNA take place (Crozet et al 1981, Brevini-Gandolfi & Gandolfi 2001. The mRNA content in oocytes is affected by animal nutrition (Pisani et al 2008), follicle diameter (Caixeta et al 2009), IVM culture media (Salhab et al 2011), in vivo and in vitro conditions (Wells & Patrizio 2008) and apoptosis (Li et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep

Catalá¹,

Izquierdo²,

Uzbekova³

et al. 2011

Reproduction

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The aim of this study was to test the Brilliant Cresyl Blue (BCB) stain to select prepubertal sheep oocytes for in vitro blastocyst production. Oocyte diameter, mitochondrial activity, maturation-promoting factor (MPF) activity and mRNA relative expression (RE) of genes related to metabolism (ATPase Na C /K C transporting a 1 (ATP1A1) and cytochrome c oxidase subunit 1 (COX1)) and constitutive function of the cell (cytoplasmic polyadenylation-element-binding protein (CPEB) and S100A10) were assessed. Immature oocytes were exposed to different BCB concentrations (13, 26, 39 and 52 mM) and classified according to their cytoplasm colouration as grown BCBC (blue cytoplasm) and growing BCBK (colourless cytoplasm). Staining oocytes with 13 mM BCB during 60 min allows selection of (BCBC) the largest (123.66 mm) and most competent oocytes to develop to the blastocyst stage (21%) with a higher number of cells (69.71G6.19S.E.M.) compared with non-stained BCBK oocytes (106.82 mm, 9% and 45.91G3.35 S.E.M. respectively). Mitochondrial activity, assessed by MitoTracker Orange CMTMRos probe, was significantly higher in BCBC than in BCBK oocytes after in vitro maturation (3369 and 1565 AU respectively). MPF activity was assessed by CDC2 kinase activity assay showing significantly higher activity at metaphase II stage in BCBC than in BCBK oocytes (1.479G0.09 and 1.184G0.05 optical density respectively). The genes analysed in this work, ATP1A1, COX1, CPEB and S100A10, did not show significant effect in mRNA RE between BCB selected oocytes. In conclusion, BCB stains larger and more competent oocytes to develop to the blastocyst stage with more active mitochondria and MPF activity and higher blastocyst cell number.

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“…Another difference between the two types of oocytes is the extent of DNA methylation, histone acetylation, and histone methylation, which is higher in SN oocytes (Kageyama et al 2007). Oocytes possessing NSN and SN chromatin organizations have been found in a variety of mammals, including rats (Mandl 1962), monkeys (Lefevre et al 1989), pigs (Crozet et al 1981), and humans (Parfenov et al 1989).…”

Section: Introductionmentioning

confidence: 99%

The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

Bellone¹,

Zuccotti²,

Redi³

et al. 2009

Reproduction

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Based on their chromatin organization, antral oocytes can be classified into two classes, namely surrounded nucleolus (SN, chromatin forms a ring around the nucleolus), and not surrounded nucleolus (NSN, chromatin has a diffuse pattern). Oocytes of both classes are capable of meiotic resumption, but while SN oocytes, following fertilization, develop to term, NSN oocytes never develop beyond the two-cell stage. A recent study has shown that the position of the germinal vesicle (GV) can be used as a morphological marker predictive of oocyte meiotic competence, i.e. oocytes with a central GV have a higher meiotic competence than oocytes with an eccentric GV. In the present study, we have associated both markers with the aim of identifying, with more accuracy, the oocytes' developmental competence. Following their isolation, antral oocytes were classified on the basis of both SN and NSN chromatin configuration and their GV position, matured to metaphase II and fertilized in vitro. We demonstrated that the position of the GV is a good marker to predict the oocytes' developmental competence, but only when associated with the observation of the chromatin organization.

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Nucleolar fine structure and RNA synthesis in bovine oocytes from antral follicles

Cited by 154 publications

References 17 publications

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep

The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

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