Nuclear pool of phosphatidylinositol 4 phosphate 5 kinase 1α is modified by polySUMO-2 during apoptosis

Chakrabarti, Rajarshi; Bhowmick, Debashis; Bhargava, Varsha; Bhar, Kaushik; Siddhanta, Anirban

doi:10.1016/j.bbrc.2013.08.058

Cited by 4 publications

(9 citation statements)

References 25 publications

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“…Previous reports from our laboratory confirmed that nuclear PIP5K is modified by SUMO-1 (12). Primary sequence analysis of PIP5K by SUMOplot TM displays six possible sites for SUMOylation, three of which (Lys-33, Lys-244, and Lys-490) have a probability score higher than 0.50 (Table 1).…”

Section: Resultsmentioning

confidence: 64%

“…Immunofluorescence and Microscopy-Immunofluorescence was done as described previously (12). Mounted cells were imaged using Nikon inverted research microscope ECLIPSE Ti-U and Olympus FV1000 LSM confocal microscope.…”

Section: Methodsmentioning

confidence: 99%

“…Cell Lysate Preparation and Western Blotting-Cell lysate preparation and Western blotting were performed as described previously (12).…”

Section: Methodsmentioning

confidence: 99%

“…PIP5K Associates with H3K9me3 during rDNA Silencing-To investigate the role of PIP5K in rDNA transcription, we monitored the occupancy pattern of modified histone H3 in the context of rRNA gene in cells expressing either wild type or kinasedead PIP5K. We chose to use kinase-dead PIP5K (PIP5KD) as a negative control because our previous studies (12) and results from other laboratories (23) have shown that this mutant fails to get translocated into the nucleus. We sought to study the relative occupancy pattern of an activation mark, H3K4me3, and a repression mark, H3K9me3, throughout the human rDNA loci using ChIP assays (Fig.…”

Section: Pip5k Accumulates In the Fibrillar Center Of The Nucleolus Wmentioning

confidence: 99%

“…Most of these studies implicated PIP 2 in mediating the aforesaid nuclear functions. Studies from our laboratory demonstrated that the nuclear pool of PIP5K is modified by SUMO-1, and during apoptotic stress in addition to the increase in this SUMOylated pool, poly-SUMO-2-modified PIP5K also occurs in the nucleus (12).…”

mentioning

confidence: 99%

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Phosphatidylinositol-4-phosphate 5-Kinase 1α Modulates Ribosomal RNA Gene Silencing through Its Interaction with Histone H3 Lysine 9 Trimethylation and Heterochromatin Protein HP1-α

Chakrabarti

Sanyal

Ghosh

et al. 2015

Journal of Biological Chemistry

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Background: Cell cycle-specific localization and nuclear function of Phosphatidylinositol-4-phosphate 5-kinase1␣ (PIP5K) is unclear. Results: SUMOylation of PIP5K at Lys-244 and Lys-490 directs its nuclear entry and its interaction with H3K9me3/HP1-␣, respectively. Conclusion: PIP5K functions as a member of the rDNA silencing complex. Significance: Our results indicate a possible novel epigenetic role of PIP5K per se in silencing of rDNA.

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Section: Resultsmentioning

confidence: 64%

Section: Methodsmentioning

confidence: 99%

“…Cell Lysate Preparation and Western Blotting-Cell lysate preparation and Western blotting were performed as described previously (12).…”

Section: Methodsmentioning

confidence: 99%

Section: Pip5k Accumulates In the Fibrillar Center Of The Nucleolus Wmentioning

confidence: 99%

mentioning

confidence: 99%

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Phosphatidylinositol-4-phosphate 5-Kinase 1α Modulates Ribosomal RNA Gene Silencing through Its Interaction with Histone H3 Lysine 9 Trimethylation and Heterochromatin Protein HP1-α

Chakrabarti

Sanyal

Ghosh

et al. 2015

Journal of Biological Chemistry

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When PIP2 Meets p53: Nuclear Phosphoinositide Signaling in the DNA Damage Response

Wang

Sheetz

2022

Front. Cell Dev. Biol.

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The mechanisms that maintain genome stability are critical for preventing tumor progression. In the past decades, many strategies were developed for cancer treatment to disrupt the DNA repair machinery or alter repair pathway selection. Evidence indicates that alterations in nuclear phosphoinositide lipids occur rapidly in response to genotoxic stresses. This implies that nuclear phosphoinositides are an upstream element involved in DNA damage signaling. Phosphoinositides constitute a new signaling interface for DNA repair pathway selection and hence a new opportunity for developing cancer treatment strategies. However, our understanding of the underlying mechanisms by which nuclear phosphoinositides regulate DNA damage repair, and particularly the dynamics of those processes, is rather limited. This is partly because there are a limited number of techniques that can monitor changes in the location and/or abundance of nuclear phosphoinositide lipids in real time and in live cells. This review summarizes our current knowledge regarding the roles of nuclear phosphoinositides in DNA damage response with an emphasis on the dynamics of these processes. Based upon recent findings, there is a novel model for p53’s role with nuclear phosphoinositides in DNA damage response that provides new targets for synthetic lethality of tumors.

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Nuclear Phosphoinositides: Their Regulation and Roles in Nuclear Functions

Fiume

Faenza

Sheth

et al. 2019

IJMS

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Polyphosphoinositides (PPIns) are a family of seven lipid messengers that regulate a vast array of signalling pathways to control cell proliferation, migration, survival and differentiation. PPIns are differentially present in various sub-cellular compartments and, through the recruitment and regulation of specific proteins, are key regulators of compartment identity and function. Phosphoinositides and the enzymes that synthesise and degrade them are also present in the nuclear membrane and in nuclear membraneless compartments such as nuclear speckles. Here we discuss how PPIns in the nucleus are modulated in response to external cues and how they function to control downstream signalling. Finally we suggest a role for nuclear PPIns in liquid phase separations that are involved in the formation of membraneless compartments within the nucleus.

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Nuclear pool of phosphatidylinositol 4 phosphate 5 kinase 1α is modified by polySUMO-2 during apoptosis

Cited by 4 publications

References 25 publications

Phosphatidylinositol-4-phosphate 5-Kinase 1α Modulates Ribosomal RNA Gene Silencing through Its Interaction with Histone H3 Lysine 9 Trimethylation and Heterochromatin Protein HP1-α

Phosphatidylinositol-4-phosphate 5-Kinase 1α Modulates Ribosomal RNA Gene Silencing through Its Interaction with Histone H3 Lysine 9 Trimethylation and Heterochromatin Protein HP1-α

When PIP2 Meets p53: Nuclear Phosphoinositide Signaling in the DNA Damage Response

Nuclear Phosphoinositides: Their Regulation and Roles in Nuclear Functions

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