Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects

Motta, Andréa; Paris, Debora; Melck, Dominique; Laurentiis, Guglielmo de; Maniscalco, Mauro; Miceli, Sofia; Montuschi, Paolo

doi:10.1183/09031936.00036411

Cited by 71 publications

(64 citation statements)

References 20 publications

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“…Recent studies examining the influence of external contaminants on EBC, including disinfectants, found that standard disinfection protocols had measurable disinfectant contaminant signal when analysed by nuclear magnetic resonance spectroscopy. However, one study showed that the disinfectant Milton showed no signals [ 100 ]. Although water soaking alone was able to effectively remove most toxic components of disinfectant, ethanol washing was required for removal of unknown contaminants which may interfere with assays [ 104 ].…”

Section: Disinfection Of Non-disposable Apparatus Componentsmentioning

confidence: 99%

“…NMR-based metabolomics of EBC may have a potential use in characterising respiratory diseases as it was able to discriminate patients with COPD [ 104 ], asthma [ 106 ], stable and unstable cystic fibrosis [ 107 ] from healthy subjects. In addition, a recent NMR spectroscopic study has also shown that the repeated use of the EcoScreen ® condenser does not cause any carry-over effect and the disinfectant Milton does not alter EBC metabolomics [ 100 ], as opposed to another study that reported artificial signals when the Anacon condenser was used and disinfected with Descogen [ 108 ]. Different analytical techniques, including NMR and mass spectro metry, may be combined to improve sensitivity and further consolidate ' breathomics ' [ 100 ].…”

Section: Nmr Spectroscopy-based Metabolomicsmentioning

confidence: 99%

“…This is a reproducible technique and its methodology has been validated [ 100 ]. Although NMR has a lower sensitivity compared to enzyme-linked immunosorbent assay (ELISA) and mass spectrometry, it is a non-destructive analytical method that requires minimal sample preparation and has rapid acquisition time of about 10 -15 min [ 104 ].…”

Section: Nmr Spectroscopy-based Metabolomicsmentioning

confidence: 99%

See 2 more Smart Citations

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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Since the late 1990s, a surge in interest in the analysis of exhaled breath condensate (EBC) resulted in the American Thoracic Society and European Respiratory Society (ATS/ERS) organising a Task Force in 2001 to develop guidelines on EBC collection and measurement of biomarkers. This Task Force published their guidelines in 2005 based on literature and expert opinions at that time, and multiple shortcomings and knowledge deficits were also identified. The clinical application of EBC collection and its biomarkers are currently still limited by several of these knowledge gaps, hence further guidelines for standardisation are required to ensure external validity. Using related articles produced since the publication of the ATS/ERS Task Force report, this paper attempts to provide a comprehensive update to the original guideline and review the methodological shortcomings identified. This review can hopefully serve as a yardstick for future studies involving this emerging clinical tool.

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Section: Disinfection Of Non-disposable Apparatus Componentsmentioning

confidence: 99%

Section: Nmr Spectroscopy-based Metabolomicsmentioning

confidence: 99%

Section: Nmr Spectroscopy-based Metabolomicsmentioning

confidence: 99%

See 1 more Smart Citation

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Alternative volatile compounds may prove better biomarkers, for example, exhaled 8-isoprostane (15,72,78). Electronic-nose and NMR spectroscopy of the exhaled breath condensate, which are reproducible and validated techniques (18,82), could be used to identify infants at a higher risk of lung injury and to personalize a pharmacologic approach to prevention and treatment of disease.…”

Section: Oxidative Stress In Bpdmentioning

confidence: 99%

Developmental Regulation of Antioxidant Enzymes and Their Impact on Neonatal Lung Disease

Berkelhamer

Farrow

2014

Antioxidants & Redox Signaling

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Significance: Deficient antioxidant defenses and compromised ability to respond to oxidative stress burden the immature lung. Routine neonatal therapies can cause increased oxidative stress with subsequent injury to the premature lung. Novel therapeutic approaches to protect the premature lung are greatly needed. Recent Advances: Live cell imaging with targeted redox probes allows for the measurement of subcellular oxidative stress and for comparisons of oxidative stress across development. Comprehension of subcellular and cell-typespecific responses to oxidative stress may influence the targeting of future antioxidant therapies. Critical Issues: Challenges remain in identifying the optimal cellular targets, degree of enzyme activity, and appropriate antioxidant therapy. Further, the efficacy of delivering exogenous antioxidants to specific cell types or subcellular compartments remains under investigation. Treatment with a nonselective antioxidant could unintentionally compromise cellular function or impact cellular defense mechanisms and homeostasis. Future Directions: Genetic and/or biomarker screening may identify infants at the greatest risk for oxidative lung injury and guide the use of more selective antioxidant therapies. Novel approaches to the delivery of antioxidant enzymes may allow cell type-or cellular organelle-specific therapy. Improved comprehension of the antioxidant enzyme regulation across cell type, cell compartment, gender, and developmental stage is critical to the design and optimization of therapy.

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“…In addition, the simplicity of sample preparation, low volume requirement (typically a few hundred microliters), non-destructive measurement, and last but not least cost efficiency led to rapid acceptance of NMR in noninvasive diagnostics (Motta et al, 2012;Ibrahim et al, 2013;Nagana Gowda and Raftery, 2015;Soininen et al, 2015). The most outstanding point is that hundreds of metabolites can be analyzed in just one NMR measurement (Duarte et al, 2014).…”

Section: Prostate Cancermentioning

confidence: 99%

Nuclear magnetic resonance spectroscopy as a new approach for improvement of early diagnosis and risk stratification of prostate cancer

Yang

Lü

Wen

et al. 2017

J. Zhejiang Univ. Sci. B

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Prostate cancer (PCa) is the second most common male cancer worldwide and the fifth leading cause of death from cancer in men. Early detection and risk stratification is the most effective way to improve the survival of PCa patients. Current PCa biomarkers lack sufficient sensitivity and specificity to cancer. Metabolite biomarkers are evolving as a new diagnostic tool. This review is aimed to evaluate the potential of metabolite biomarkers for early detection, risk assessment, and monitoring of PCa. Of the 154 identified publications, 27 and 38 were original papers on urine and serum metabolomics, respectively. Nuclear magnetic resonance (NMR) is a promising method for measuring concentrations of metabolites in complex samples with good reproducibility, high sensitivity, and simple sample processing. Especially urine-based NMR metabolomics has the potential to be a cost-efficient method for the early detection of PCa, risk stratification, and monitoring treatment efficacy.

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Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects

Cited by 71 publications

References 20 publications

Exhaled breath condensate: a comprehensive update

Exhaled breath condensate: a comprehensive update

Developmental Regulation of Antioxidant Enzymes and Their Impact on Neonatal Lung Disease

Nuclear magnetic resonance spectroscopy as a new approach for improvement of early diagnosis and risk stratification of prostate cancer

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