Nuclear Factor I Regulates Brain Fatty Acid-Binding Protein and Glial Fibrillary Acidic Protein Gene Expression in Malignant Glioma Cell Lines

Brun, Miranda; Coles, Jeffrey E.; Monckton, Elizabeth A.; Glubrecht, Darryl; Bisgrove, Dwayne A.; Godbout, Roseline

doi:10.1016/j.jmb.2009.06.041

Cited by 58 publications

(84 citation statements)

References 63 publications

(77 reference statements)

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“…Based on chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays, NFI binds to all three NFI consensus sites in both genes. In addition, we have shown that modulation of NFI expression alters B-FABP and GFAP promoter activity, as well as endogenous expression of B-FABP and GFAP in MG cell lines (16). Our data indicate that NFI is differentially phosphorylated in different MG cell lines and that NFI phosphorylation state correlates with expression of B-FABP and GFAP, i.e.…”

mentioning

confidence: 57%

“…Our laboratory has demonstrated that specific NFIs have distinct effects on NFI-dependent promoter activity (16). Furthermore, NFIs can either activate or repress transcription from NFI-dependent promoters, and regulation by NFI is both tissue-and promoter context-dependent (16,20).…”

mentioning

confidence: 96%

“…Expression of B-FABP and GFAP in MG cells is regulated by nuclear factor I (NFI) (16,17). The NFI family of transcription factors consists of the four genes NFIA, NFIB, NFIC, and NFIX, all of which can bind to the consensus binding site 5Ј-TTGGCN 5 GCCAA-3Ј as a homodimer or heterodimer to regulate target gene expression (18,19).…”

mentioning

confidence: 99%

“…The B-FABP and GFAP promoters each contain three NFI consensus binding sites (16,17,32). Based on chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays, NFI binds to all three NFI consensus sites in both genes.…”

mentioning

confidence: 99%

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Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines

Brun¹,

Glubrecht²,

Baksh

et al. 2013

Journal of Biological Chemistry

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Background: Nuclear factor I (NFI) phosphorylation controls the expression of glial genes associated with migration. Results: NFI is dephosphorylated and activated by a cleaved form of calcineurin in malignant glioma cells. Conclusion: NFI transcriptional activity in malignant glioma is regulated in part by calcineurin. Significance: Regulation of NFI by calcineurin provides a novel approach to control the expression of genes associated with migration/infiltration in malignant glioma.

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confidence: 57%

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confidence: 96%

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confidence: 99%

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confidence: 99%

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Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines

Brun¹,

Glubrecht²,

Baksh

et al. 2013

Journal of Biological Chemistry

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“…Among these are the Nuclear factor I (NFI) transcription factors, with NFIA/B/X upregulating FABP7, and NFIC down-regulating FABP7 in malignant glioma cell lines [65].…”

Section: Fabp7 and Its Transcriptional Regulatorsmentioning

confidence: 99%

Interaction of brain fatty acid-binding protein with the polyunsaturated fatty acid environment as a potential determinant of poor prognosis in malignant glioma

Elsherbiny

Emara

Godbout

2013

Progress in Lipid Research

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Malignant gliomas are the most common adult brain cancers. In spite of aggressive treatment, recurrence occurs in the great majority of patients and is invariably fatal. Polyunsaturated fatty acids are abundant in brain, particularly ω-6 arachidonic acid (AA) and ω-3 docosahexaenoic acid (DHA). Although the levels of ω-6 and ω-3 polyunsaturated fatty acids are tightly regulated in brain, the ω-6:ω-3 ratio is dramatically increased in malignant glioma, suggesting deregulation of fundamental lipid homeostasis in brain tumor tissue. The migratory properties of malignant glioma cells can be modified by altering the ratio of AA:DHA in growth medium, with increased migration observed in AA-rich medium. This fatty acid-dependent effect on cell migration is dependent on expression of the brain fatty acid binding protein (FABP7) previously shown to bind DHA and AA. Increased levels of enzymes involved in eicosanoid production in FABP7-positive malignant glioma cells suggest that FABP7 is an important modulator of AA metabolism. We provide evidence that increased production of eicosanoids in FABP7-positive malignant glioma growing in an AA-rich environment contributes to tumor infiltration in the brain. We discuss pathways and molecules that may underlie FABP7/AA-mediated promotion of cell migration and FABP7/DHA-mediated inhibition of cell migration in malignant glioma.

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Variants in nuclear factor I genes influence growth and development

Zenker

Bunt

Schanze

et al. 2019

American J of Med Genetics Pt C

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Nuclear Factor I Regulates Brain Fatty Acid-Binding Protein and Glial Fibrillary Acidic Protein Gene Expression in Malignant Glioma Cell Lines

Cited by 58 publications

References 63 publications

Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines

Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines

Interaction of brain fatty acid-binding protein with the polyunsaturated fatty acid environment as a potential determinant of poor prognosis in malignant glioma

Variants in nuclear factor I genes influence growth and development

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