Nuclear envelope associated protein that binds telomeric DNAs

A Telomere-Binding Protein (TRF2/MTBP) from Mouse Nuclear Matrix with Motives of an Intermediate Filament-Type Rod Domain

“…The immunological results presented here show that the frog oocyte nuclear envelope protein MTBP, which was earlier shown to bind specifically to telomeric DNA in vitro, 22 is identical to the previously identified telomeric DNA-binding protein TRF2, 15,16 and we have therefore termed this protein MTBP/TRF2. TRF2 appears to control the structure of the very end of the chromosomal DNA molecule.…”

“…To examine the localisation of MTBP/TRF2 in mitotic mouse cells, Centrospin preparation of cultured cells treated with colcemid were probed with affinity-purified anti-MTBP antibody and by in situ hybridisation with the (T 2 AG 3 ) 135 telomeric DNA probe; the signals from these probes are co-localised in interphase nuclei. 22,53 On metaphase chromosomes, part of the antibody signal was seen at the ends of the chromosomes (Fig. 6I.a) like that seen with antibody against TRF2.…”

“…MTBP/TRF2 appears to be predominantly a nuclear envelope protein; it is localized in the outer region of the nuclei of frog blood cells 47 and on the envelope of frog oocyte nuclei. 22 In the isolated mouse liver nuclei examined here, it was located close to the nuclear envelope rather than distributed randomly or according to the Rabl model (Fig. 6IV).…”

“…AB against MTBP do not stain TRF2 myb-containing fusion protein for its antigenic determinant does not overlap with myb-domain. 22 Parts of TRF2/MTBP similarity to the rod-domain also lie outside myb-domain (Fig. 7).…”

A Telomere-Binding Protein (TRF2/MTBP) from Mouse Nuclear Matrix with Motives of an Intermediate Filament-Type Rod Domain

“…The immunological results presented here show that the frog oocyte nuclear envelope protein MTBP, which was earlier shown to bind specifically to telomeric DNA in vitro, 22 is identical to the previously identified telomeric DNA-binding protein TRF2, 15,16 and we have therefore termed this protein MTBP/TRF2. TRF2 appears to control the structure of the very end of the chromosomal DNA molecule.…”

“…To examine the localisation of MTBP/TRF2 in mitotic mouse cells, Centrospin preparation of cultured cells treated with colcemid were probed with affinity-purified anti-MTBP antibody and by in situ hybridisation with the (T 2 AG 3 ) 135 telomeric DNA probe; the signals from these probes are co-localised in interphase nuclei. 22,53 On metaphase chromosomes, part of the antibody signal was seen at the ends of the chromosomes (Fig. 6I.a) like that seen with antibody against TRF2.…”

“…MTBP/TRF2 appears to be predominantly a nuclear envelope protein; it is localized in the outer region of the nuclei of frog blood cells 47 and on the envelope of frog oocyte nuclei. 22 In the isolated mouse liver nuclei examined here, it was located close to the nuclear envelope rather than distributed randomly or according to the Rabl model (Fig. 6IV).…”

“…AB against MTBP do not stain TRF2 myb-containing fusion protein for its antigenic determinant does not overlap with myb-domain. 22 Parts of TRF2/MTBP similarity to the rod-domain also lie outside myb-domain (Fig. 7).…”

Dynamics of satellite binding protein CENP‐B and telomere binding protein TRF2/MTBP in the nuclei of mouse spermatogenic line

“…TEL binding to the membrane is often observed in morphological studies (Comings, 1980). A series of studies in our laboratory prove that it is TRF2 that provides this connection (Bugaeva and Podgornaya, 1997;Voronin et al, 1999;Podgornaya et al, 2000;Dolnik et al, 2003;Voronin et al, 2003).…”

A Telomere-Binding Protein (TRF2/MTBP) from Mouse Nuclear Matrix with Motives of an Intermediate Filament-Type Rod Domain

“…The immunological results presented here show that the frog oocyte nuclear envelope protein MTBP, which was earlier shown to bind specifically to telomeric DNA in vitro, 22 is identical to the previously identified telomeric DNA-binding protein TRF2, 15,16 and we have therefore termed this protein MTBP/TRF2. TRF2 appears to control the structure of the very end of the chromosomal DNA molecule.…”

“…To examine the localisation of MTBP/TRF2 in mitotic mouse cells, Centrospin preparation of cultured cells treated with colcemid were probed with affinity-purified anti-MTBP antibody and by in situ hybridisation with the (T 2 AG 3 ) 135 telomeric DNA probe; the signals from these probes are co-localised in interphase nuclei. 22,53 On metaphase chromosomes, part of the antibody signal was seen at the ends of the chromosomes (Fig. 6I.a) like that seen with antibody against TRF2.…”

“…MTBP/TRF2 appears to be predominantly a nuclear envelope protein; it is localized in the outer region of the nuclei of frog blood cells 47 and on the envelope of frog oocyte nuclei. 22 In the isolated mouse liver nuclei examined here, it was located close to the nuclear envelope rather than distributed randomly or according to the Rabl model (Fig. 6IV).…”

“…AB against MTBP do not stain TRF2 myb-containing fusion protein for its antigenic determinant does not overlap with myb-domain. 22 Parts of TRF2/MTBP similarity to the rod-domain also lie outside myb-domain (Fig. 7).…”

Dynamics of satellite binding protein CENP‐B and telomere binding protein TRF2/MTBP in the nuclei of mouse spermatogenic line

“…TEL binding to the membrane is often observed in morphological studies (Comings, 1980). A series of studies in our laboratory prove that it is TRF2 that provides this connection (Bugaeva and Podgornaya, 1997;Voronin et al, 1999;Podgornaya et al, 2000;Dolnik et al, 2003;Voronin et al, 2003).…”

A Telomere-Binding Protein (TRF2/MTBP) from Mouse Nuclear Matrix with Motives of an Intermediate Filament-Type Rod Domain

“…The immunological results presented here show that the frog oocyte nuclear envelope protein MTBP, which was earlier shown to bind specifically to telomeric DNA in vitro, 22 is identical to the previously identified telomeric DNA-binding protein TRF2, 15,16 and we have therefore termed this protein MTBP/TRF2. TRF2 appears to control the structure of the very end of the chromosomal DNA molecule.…”

“…To examine the localisation of MTBP/TRF2 in mitotic mouse cells, Centrospin preparation of cultured cells treated with colcemid were probed with affinity-purified anti-MTBP antibody and by in situ hybridisation with the (T 2 AG 3 ) 135 telomeric DNA probe; the signals from these probes are co-localised in interphase nuclei. 22,53 On metaphase chromosomes, part of the antibody signal was seen at the ends of the chromosomes (Fig. 6I.a) like that seen with antibody against TRF2.…”

“…MTBP/TRF2 appears to be predominantly a nuclear envelope protein; it is localized in the outer region of the nuclei of frog blood cells 47 and on the envelope of frog oocyte nuclei. 22 In the isolated mouse liver nuclei examined here, it was located close to the nuclear envelope rather than distributed randomly or according to the Rabl model (Fig. 6IV).…”

“…AB against MTBP do not stain TRF2 myb-containing fusion protein for its antigenic determinant does not overlap with myb-domain. 22 Parts of TRF2/MTBP similarity to the rod-domain also lie outside myb-domain (Fig. 7).…”

Dynamics of satellite binding protein CENP‐B and telomere binding protein TRF2/MTBP in the nuclei of mouse spermatogenic line

“…TEL binding to the membrane is often observed in morphological studies (Comings, 1980). A series of studies in our laboratory prove that it is TRF2 that provides this connection (Bugaeva and Podgornaya, 1997;Voronin et al, 1999;Podgornaya et al, 2000;Dolnik et al, 2003;Voronin et al, 2003).…”