Nuclear DNA Content Variation among Central European Koeleria Taxa

Pečinka, Aleš; Suchánková, Pavla; Lysak, Martin A.; Trávníček, Bohumil; Doležel, Jaroslav

doi:10.1093/aob/mcl077

Cited by 35 publications

(30 citation statements)

References 30 publications

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“…The decrease in the size of polyploidy genomes in comparison with a correspondent diploid was also observed in other species (Baack et al 2005, Pecinka et al 2006, but mechanisms that lead to DNA loss are still poorly understood. These phenomena can occur due to genome rearrangements, transposon activations, elimination of specific sequences and unequal homologous recombination (Leitch and Bennett 2004).…”

Section: Discussionmentioning

confidence: 77%

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Pierre

Sousa

Davide

et al. 2011

An. Acad. Bras. Ciênc.

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Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of mixoploidy. Our data indicates that sexual reproduction among those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.

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Section: Discussionmentioning

confidence: 77%

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Pierre

Sousa

Davide

et al. 2011

An. Acad. Bras. Ciênc.

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“…23,28,29) We investigated the nucleic DNA contents of several yeast strains using FACS, and calculated differences in DNA contents between the G1 phase and the G2/M phase ( Table 2). The DNA contents of S. cerevisiae cells (haploid, diploid, and tetraploid) tended to increase in proportion to their ploidy.…”

Section: Multiple Disruption Of Cuura3mentioning

confidence: 99%

Efficient Gene Disruption in the High-Ploidy YeastCandida utilisUsing the Cre-loxPSystem

Ikushima¹,

Fujii²,

Kobayashi³

2009

Bioscience, Biotechnology, and Biochemistry

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“…Koeleria capensis has been reported as diploid (2n = 14) in six eastern African mountain areas (the Ethiopian Simen, Galama, and Bale Mts, and the East African Mt Kenya, Mt Kilimanjaro, and Cherangani Hills), and one tetraploid count (2n = 28) has been reported from Mt Kilimanjaro (Hedberg and Hedberg 1977). Polyploidy is common in European Koeleria taxa (Pecinka et al 2006). Koeleria capensis is a polymorphic species showing large variation in spikelet size, plant compactness, and stiffness and pubescence of the leaves (Phillips 1995).…”

Section: Introductionmentioning

confidence: 99%

Phylogeographic history and taxonomy of some afro-alpine grasses assessed based on AFLPs and morphometry: Deschampsia cespitosa, D. angusta and Koeleria capensis

et al. 2013

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Phylogeographic studies in the high mountains of Africa are hampered by the limited material available, resulting in insufficient knowledge of taxonomic variation within and among closely related species. Here we address genetic and morphological variation in three grass species, of which one (Deschampsia angusta) has been reported as narrowly endemic and vulnerable whereas D. cespitosa and Koeleria capensis are widely distributed also outside the afro-alpine region. We used Amplified Fragment Length Polymorphisms (AFLPs) to assess genetic structuring and diversity in material collected during recent field expeditions and included additional herbarium material in morphometric analyses. The plants identified as the endemic D. angusta were genetically very similar to those identified as D. cespitosa from the same mountain (Mt Ruwenzori), forming a single coherent genetic group in STRUCTURE analysis. The plants identified as D. angusta seem to represent extremes of continuous gradients of morphological variation within a single, variable species, D. cespitosa. We found that the afro-alpine material of Deschampsia consists of three genetically very distinct groups corresponding to the three mountains investigated, suggesting persistence in isolated afroalpine refugia during one or more glacial cycles. In contrast, we found no clear genetic structure in K. capensis. This species harboured very little genetic diversity in all six mountain areas examined, and little genetic rarity except in the Ethiopian Simen Mts. This pattern may be explained by recent colonization of the afro-alpine region from a single source population or possibly by extensive recent gene flow combined with bottlenecks. We found however some differentiation between different K. capensis populations from Mt Kilimanjaro, corresponding to two described varieties. This study demonstrates the need for further taxonomic exploration of the enigmatic flora of the isolated afro-alpine 'sky islands' and highlights that different species may have conspicuously different phylogeographic histories.

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Nuclear DNA Content Variation among Central European Koeleria Taxa

Cited by 35 publications

References 30 publications

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Efficient Gene Disruption in the High-Ploidy YeastCandida utilisUsing the Cre-loxPSystem

Phylogeographic history and taxonomy of some afro-alpine grasses assessed based on AFLPs and morphometry: Deschampsia cespitosa, D. angusta and Koeleria capensis

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