Nuclear Architecture and Dynamics: Territories, Nuclear Bodies, and Nucleocytoplasmic Trafficking

Cheung, Alice Y.; Reddy, Anireddy S. N.

doi:10.1104/pp.111.900426

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…Figure (b) shows that AG‐incRNA4 and COLDAIR co‐localized with CLF and formed nuclear speckles. These results implied that AG‐incRNA/ CLF and COLDAIR /CLF might form complexes comprising proteins and RNAs from the surrounding nucleoplasm (Cheung & Reddy, ). By contrast, the nonfunctional AG‐incRNA1 showed similar result as RSM1 , which served as a negative control (Fig.…”

Section: Resultsmentioning

confidence: 99%

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

Deng

et al. 2018

New Phytologist

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Dispersed H3K27 trimethylation (H3K27me3) of the AGAMOUS (AG) genomic locus is mediated by CURLY LEAF (CLF), a component of the Polycomb Repressive Complex (PRC) 2. Previous reports have shown that the AG second intron, which confers AG tissue-specific expression, harbors sequences targeted by several positive and negative regulators. Using RACE reverse transcription polymerase chain reaction, we found that the AG intron 2 encodes several noncoding RNAs. RNAi experiment showed that incRNA4 is needed for CLF repressive activity. AG-incRNA4RNAi lines showed increased leaf AG mRNA levels associated with a decrease of H3K27me3 levels; these plants displayed AG overexpression phenotypes. Genetic and biochemical analyses demonstrated that the AG-incRNA4 can associate with CLF to repress AG expression in leaf tissues through H3K27me3-mediated repression and to autoregulate its own expression level. The mechanism of AG-incRNA4-mediated repression may be relevant to investigations on tissue-specific expression of Arabidopsis MADS-box genes.

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Section: Resultsmentioning

confidence: 99%

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

Deng

et al. 2018

New Phytologist

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“…Studies of nuclear architecture and dynamics have been emerging as hot topics in plant science (Cheung and Reddy, ). The nucleoplasm is organized into non‐membrane‐bound subdomains and bodies, which play diverse roles in DNA replication, gene expression, gene silencing, proteolysis and RNA splicing (Mao et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a soybean nuclear localized type–III DnaJ domain‐containing HSP40 reveals its roles in cell death and disease resistance

2013

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SUMMARYHeat-shock proteins such as HSP70 and HSP90 are important molecular chaperones that play critical roles in biotic and abiotic stress responses; however, the involvement of their co-chaperones in stress biology remains largely uninvestigated. In a screen for candidate genes stimulating cell death in Glycine max (soybean), we transiently overexpressed full-length cDNAs of soybean genes that are highly induced during soybean rust infection in Nicotiana benthamiana leaves. Overexpression of a type-III DnaJ domain-containing HSP40 (GmHSP40.1), a co-chaperone of HSP70, caused hypersensitive response (HR)-like cell death. The HRlike cell death was dependent on MAPKKKa and WIPK, because silencing each of these genes suppressed the HR. Consistent with the presence of a nuclear localization signal (NLS) motif within the GmHSP40.1 coding sequence, GFP-GmHSP40.1 was exclusively present in nuclear bodies or speckles. Nuclear localization of GmHSP40.1 was necessary for its function, because deletion of the NLS or addition of a nuclear export signal abolished its HR-inducing ability. GmHSP40.1 co-localized with HcRed-SE, a protein involved in pri-miRNA processing, which has been shown to be co-localized with SR33-YFP, a protein involved in pre-mRNA splicing, suggesting a possible role for GmHSP40.1 in mRNA splicing or miRNA processing, and a link between these processes and cell death. Silencing GmHSP40.1 enhanced the susceptibility of soybean plants to Soybean mosaic virus, confirming its positive role in pathogen defense. Together, the results demonstrate a critical role of a nuclear-localized DnaJ domain-containing GmHSP40.1 in cell death and disease resistance in soybean.

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“…It serves as the regulator in cell signaling for perceiving and transmitting extra- and inter-cellular signals in many cellular pathways. Communication between the cytoplasm and the nucleus is necessary and evident because of events such as apoptosis (Broers et al, 2002), mechanical stress (Dahl et al, 2008), environmental perturbation (Cheung and Reddy, 2012) and pathogen infection (Rivas, 2012), which lead to altered biosynthesis and modification of nuclear architecture and downstream cytoplasmic events. In addition, nucleoskeleton acts as a substrate for genome partitioning during mitosis.…”

Section: Introductionmentioning

confidence: 99%

Comparative analyses of nuclear proteome: extending its function

Narula¹,

Datta²,

Chakraborty³

et al. 2013

Front. Plant Sci.

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Organeller proteomics is an emerging technology that is critical in determining the cellular signal transduction pathways. Nucleus, the regulatory hub of the eukaryotic cell is a dynamic system and a repository of various macromolecules that serve as modulators of such signaling that dictate cell fate decisions. Nuclear proteins (NPs) are predicted to comprise about 10–20% of the total cellular proteins, suggesting the involvement of the nucleus in a number of diverse functions. Indeed, NPs constitute a highly organized but complex network that plays diverse roles during development and physiological processes. In plants, relatively little is known about the nature of the molecular components and mechanisms involved in coordinating NP synthesis, their action and function. Proteomic study hold promise to understand the molecular basis of nuclear function using an unbiased comparative and differential approach. We identified a few hundred proteins that include classical and non-canonical nuclear components presumably associated with variety of cellular functions impinging on the complexity of nuclear proteome. Here, we review the nuclear proteome based on our own findings, available literature, and databases focusing on detailed comparative analysis of NPs and their functions in order to understand how plant nucleus works. The review also shed light on the current status of plant nuclear proteome and discusses the future prospect.

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Nuclear Architecture and Dynamics: Territories, Nuclear Bodies, and Nucleocytoplasmic Trafficking

Cited by 8 publications

References 18 publications

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

Overexpression of a soybean nuclear localized type–III DnaJ domain‐containing HSP40 reveals its roles in cell death and disease resistance

Comparative analyses of nuclear proteome: extending its function

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