NtMYB3, an R2R3-MYB from Narcissus, Regulates Flavonoid Biosynthesis

Anwar, Muhammad; Yu, Wenbao; Hong, Ying; Zhou, Ping; Allan, Andrew C.; Zeng, Lihui

doi:10.3390/ijms20215456

Cited by 48 publications

(49 citation statements)

References 36 publications

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“…AtMYB7 ( A. thaliana ) and FaMYB1 ( Fragaria × ananassa ) were identified and found to downregulate anthocyanin biosynthesis [ 37 , 143 ]. The proposed phylogeny ( Figure 4 ), for R2R3-MYB-TF, suggests two ways to regulate flavonoid metabolism: AtMYB4-like (direct binding) [ 74 , 92 , 99 ] and FaMYB1-like (combined with other proteins) [ 44 , 74 , 104 ].…”

Section: Transcriptional Regulation Mechanism Of Anthocyanins By Mmentioning

confidence: 99%

MYB-Mediated Regulation of Anthocyanin Biosynthesis

Yan

Pei

Zhang

et al. 2021

IJMS

211

140

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Anthocyanins are natural water-soluble pigments that are important in plants because they endow a variety of colors to vegetative tissues and reproductive plant organs, mainly ranging from red to purple and blue. The colors regulated by anthocyanins give plants different visual effects through different biosynthetic pathways that provide pigmentation for flowers, fruits and seeds to attract pollinators and seed dispersers. The biosynthesis of anthocyanins is genetically determined by structural and regulatory genes. MYB (v-myb avian myeloblastosis viral oncogene homolog) proteins are important transcriptional regulators that play important roles in the regulation of plant secondary metabolism. MYB transcription factors (TFs) occupy a dominant position in the regulatory network of anthocyanin biosynthesis. The TF conserved binding motifs can be combined with other TFs to regulate the enrichment and sedimentation of anthocyanins. In this study, the regulation of anthocyanin biosynthetic mechanisms of MYB-TFs are discussed. The role of the environment in the control of the anthocyanin biosynthesis network is summarized, the complex formation of anthocyanins and the mechanism of environment-induced anthocyanin synthesis are analyzed. Some prospects for MYB-TF to modulate the comprehensive regulation of anthocyanins are put forward, to provide a more relevant basis for further research in this field, and to guide the directed genetic modification of anthocyanins for the improvement of crops for food quality, nutrition and human health.

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Section: Transcriptional Regulation Mechanism Of Anthocyanins By Mmentioning

confidence: 99%

MYB-Mediated Regulation of Anthocyanin Biosynthesis

Yan

Pei

Zhang

et al. 2021

IJMS

211

140

View full text Add to dashboard Cite

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“…AtMYB12 and AtMYB111 can control flavonoid biosynthesis in Arabidopsis (Stracke et al, 2007). Narcissus R2R3-MYB transcription factor gene NtMYB3 can regulate flavonoid biosynthesis (Anwar et al, 2019). FeMYBF1 regulates flavonoid biosynthesis in buckwheat (Matsui et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Poplar R2R3-MYB Gene Family and Over-Expression of PsnMYB108 Confers Salt Tolerance in Transgenic Tobacco

et al. 2020

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The MYB, one of the largest transcription factor families in plants, is related to various biological processes. For an example, the R2R3-MYB family plays an important role in regulation of primary and secondary metabolism, plant growth and development, and responses to hormones and stresses. However, functional studies on the poplar R2R3-MYB genes are limited. In this study, we identified 207 poplar R2R3-MYB genes that are unevenly distributed on the 19 chromosomes of poplar, followed by characterization of their conserved domains. On the basis of phylogenetic analysis, these genes can be divided into 23 groups. Evidence from synteny analyses indicated that the poplar R2R3-MYB gene family is featured by tandem and segmental duplication events. On the basis of RNA-Seq data, we investigated salt responsive genes and explored their expression patterns. Furthermore, we cloned the PsnMYB108 gene from poplar, which is significantly up-regulated in roots and leaves in response to salt stress. To validate its function, we developed transgenic tobacco plants that over-express the PsnMYB108 gene. It appears that the transgenic lines are more tolerant to salt stress than the wild type does. Evidence from physiological analyses demonstrated that over-expression of PsnMYB108 may improve tobacco salt stress tolerance by increasing the reactive oxygen species scavenging ability and the accumulation of proline. These results laid the foundation for future analysis and functional studies of poplar R2R3-MYB family members, and revealed that PsnMYB108 plays an important role in improving plant salt stress tolerance.

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“…In the Chinese narcissus ( Narcissus tazetta L. var. chinensis ), NtMYB3 induces PA accumulation by repressing flavonol biosynthesis ( Anwar et al, 2019 ). In Medicago truncatula , MtPAR promotes PA accumulation by directly repressing isoflavone biosynthesis and by redirecting the flux of the immediate precursors into the PA biosynthesis branch ( Li et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, MYB TFs with opposite effects on different biosynthesis branches of flavonoids have been observed in other plant species recently, such as Narcissus tazetta NtMYB3, Medicago truncatula MtPAR, and Camellia sinensis CsMYB5a. All of these TFs promote PA biosynthesis but repress other flavonoid biosynthesis branches, such as flavonols, isoflavones, and anthocyanins ( Li et al, 2016 ; Jiang et al, 2018 ; Anwar et al, 2019 ). In grape berries, TFs that have opposite regulatory mechanisms to PA and anthocyanin pathways are being identified.…”

Section: Introductionmentioning

confidence: 99%

Transcription Factor VviMYB86 Oppositely Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Grape Berries

Cheng

Shi

et al. 2021

Front. Plant Sci.

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Proanthocyanidins (PAs) and anthocyanins are two vital groups of flavonoid compounds for grape berries and red wines. Several transcription factors (TFs) have been identified to be involved in regulating PA and anthocyanin biosynthesis in grape berries. However, research on TFs with different regulatory mechanisms for these two biosynthesis branches in grapes remains limited. In this study, we identified an R2R3-MYB TF, VviMYB86, whose spatiotemporal gene expression pattern in grape berries coincided well with PA accumulation but contrasted with anthocyanin synthesis. Both in vivo and in vitro experiments verified that VviMYB86 positively regulated PA biosynthesis, primarily by upregulating the expression of the two leucoanthocyanidin reductase (LAR) genes in the Arabidopsis protoplast system, as well as in VviMYB86-overexpressing grape callus cultured under 24 h of darkness. Moreover, VviMYB86 was observed to repress the anthocyanin biosynthesis branch in grapes by downregulating the transcript levels of VviANS and VviUFGT. Overall, VviMYB86 is indicated to have a broad effect on flavonoid synthesis in grape berries. The results of this study will help elucidate the regulatory mechanism governing the expression of the two LAR genes in grape berries and provide new insights into the regulation of PA and anthocyanin biosynthesis in grape berries.

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NtMYB3, an R2R3-MYB from Narcissus, Regulates Flavonoid Biosynthesis

Cited by 48 publications

References 36 publications

MYB-Mediated Regulation of Anthocyanin Biosynthesis

MYB-Mediated Regulation of Anthocyanin Biosynthesis

Characterization of the Poplar R2R3-MYB Gene Family and Over-Expression of PsnMYB108 Confers Salt Tolerance in Transgenic Tobacco

Transcription Factor VviMYB86 Oppositely Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Grape Berries

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