NS3 of Bluetongue Virus Interferes with the Induction of Type I Interferon

Chauveau, Emilie; Doceul, Virginie; Lara, Estelle; Bréard, Emmanuel; Sailleau, Corinne; Vidalain, Pierre‐Olivier; Meurs, Éliane; Dabo, Stéphanie; Schwartz-Cornil, Isabelle; Zientara, Stéphan; Vitour, Damien

doi:10.1128/jvi.00678-13

Cited by 61 publications

(76 citation statements)

References 34 publications

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“…In a previous study, we have shown that BTV modulates IFN-I synthesis in epithelial cells and that this inhibition is mediated by the viral protein NS3 that interferes with the IFN-I synthesis pathway downstream of RIG-I and upstream of TBK1/IKKε activation (38). In the present study, we report novel findings showing that BTV has evolved additional strategies to interfere with the IFN-I system by modulating IFN-I signaling and the JAK/ STAT pathway in epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that most viruses are able to counteract the IFN system in order to establish an infection. Our group has shown recently that BTV is able to modulate the IFN-I production pathway (38). This inhibition depends on the viral protein NS3 that interferes with the IFN-I synthesis pathway downstream of RIG-I and upstream of TBK1/IKKε activation (38).…”

mentioning

confidence: 99%

“…Our group has shown recently that BTV is able to modulate the IFN-I production pathway (38). This inhibition depends on the viral protein NS3 that interferes with the IFN-I synthesis pathway downstream of RIG-I and upstream of TBK1/IKKε activation (38). However, nothing is known about the ability of BTV to modulate the antiviral response triggered by the secretion of IFN-I.…”

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confidence: 99%

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Dual Modulation of Type I Interferon Response by Bluetongue Virus

Doceul

Chauveau

Lara

et al. 2014

J Virol

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Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) virus that causes an economically important disease in ruminants. BTV infection is a strong inducer of type I interferon (IFN-I) in multiple cell types. It has been shown recently that BTV and, more specifically, the nonstructural protein NS3 of BTV are able to modulate the IFN-I synthesis pathway. However, nothing is known about the ability of BTV to counteract IFN-I signaling. Here, we investigated the effect of BTV on the IFN-I response pathway and, more particularly, the Janus tyrosine kinase (JAK)/signal transducer and activator of transcription protein (STAT) signaling pathway. We found that BTV infection triggered the expression of IFN-stimulated genes (ISGs) in A549 cells. However, when BTV-infected cells were stimulated with external IFN-I, we showed that activation of the IFN-stimulated response element (ISRE) promoter and expression of ISGs were inhibited. We found that this inhibition involved two different mechanisms that were dependent on the time of infection. After overnight infection, BTV blocked specifically the phosphorylation and nuclear translocation of STAT1. This inhibition correlated with the redistribution of STAT1 in regions adjacent to the nucleus. At a later time point of infection, BTV was found to interfere with the activation of other key components of the JAK/STAT pathway and to induce the downregulation of JAK1 and TYK2 protein expression. Overall, our study indicates for the first time that BTV is able to interfere with the JAK/STAT pathway to modulate the IFN-I response. IMPORTANCE Bluetongue virus (BTV) causes a severe disease in ruminants and has an important impact on the livestock economy in areas of

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Dual Modulation of Type I Interferon Response by Bluetongue Virus

Doceul

Chauveau

Lara

et al. 2014

J Virol

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“…However, viral non-structural (NS) proteins NS3 and NS4 have been described to be IFN antagonists (Chauveau et al, 2013;Ratinier et al, 2011Ratinier et al, , 2014. Lack of these proteins in a vaccine might lead to better induction of immunity.…”

Section: Onset Of Immunitymentioning

confidence: 99%

“…NS3/NS3a is involved in virus release, in particular from Culicoides cells (French et al, 1989;Guirakhoo et al, 1995;Wechsler & McHolland, 1988;Wechsler et al, 1989), and is also an IFN antagonist (Chauveau et al, 2013). van Gennip et al (2014) showed that NS3/NS3a expression is not essential for BTV replication in vitro.…”

Section: Disabled Infectious Single-animal (Disa) Vaccinementioning

confidence: 99%

Current and next-generation bluetongue vaccines: Requirements, strategies, and prospects for different field situations

Feenstra

Rijn

2016

Critical Reviews in Microbiology

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Orbiviruses and Bluetongue Virus

Mertens

Jaafar

Attoui

2015

Encyclopedia of Life Sciences

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Bluetongue virus (BTV) is the type species of the genus Orbivirus within the family Reoviridae (the largest double‐stranded ribonucleic acid (dsRNA) virus family). BTV is an arthropod‐borne pathogen that is transmitted by the bites of its vector, the Culicoides biting midges, which transmit the virus between its ruminant hosts. The virus can infect most species of domestic and wild ruminants. BT clinical signs are most common in sheep and some species of deer. In recent years, the distribution of BTV has changed considerably, particularly in Europe. New outbreaks have occurred each year from 1998 in southern and central Europe, involving several strains from 10 different serotypes. In 2006, BTV serotype 8 caused an outbreak approximately 5° further north in Europe than ever before, infecting ruminants in a wide area across northern Europe. A new virus ( Toggenburg virus ) has been identified in goats from Switzerland in 2008 and designated as the twenty‐fifth serotype of BTV and another one isolated from goats in Corsica in France more recently and was designated as serotype 27. Additional serotypes have been detected/isolated, bringing now the number of serotypes to 29. Key Concepts Genus Orbivirus is the most diverse genus of family Reoviridae containing 32 virus species. Bluetongue disease affects mainly sheep causing severe clinical signs and high mortality. Bluetongue virus is transmitted by competent species of Culicoides midges. Phylogenetic analysis identifies two major groups of orbiviruses, those with their subcore shell protein (T2 protein) being the VP2 and those with the T2 being VP3. Orbiviruses are nonturreted members of family Reoviridae , and the atomic structure of BTV core has been determined.

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NS3 of Bluetongue Virus Interferes with the Induction of Type I Interferon

Cited by 61 publications

References 34 publications

Dual Modulation of Type I Interferon Response by Bluetongue Virus

Dual Modulation of Type I Interferon Response by Bluetongue Virus

Current and next-generation bluetongue vaccines: Requirements, strategies, and prospects for different field situations

Orbiviruses and Bluetongue Virus

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