NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

Abstract

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

Comparison of dengue and other febrile illnesses in patients admitted to Teaching Hospital- Peradeniya

“…The study conducted in Bangladesh mentioned that DEN 2 serotype was most prevalent in their population 9 .…”

“…Similar dengue symptoms as fever, headache, joint pain, muscle pain and rash are observed in other febrile illnesses as Chikungunya/Zika viral infections 8 . These infections are also transmitted by the dengue vector, Aedesaegypti and Aedes albopictus 9 .…”

Dengue Serotypes in Bangladesh: Whole Genome Sequencing and Comparative Genomics Facilitates Pathogenesis and Epidemiology Studies and Informs Improved Disease Control

“…In 2018, clinical incidence of dengue peaked at approximately 6,500 cases, apparently due to circulation of the DENV-3 serotype in the country, which had been notably absent during 2013-2017 [43]. A recently published study that examined dengue isolates over the three-year period 2015-2017 confirmed co-circulation of DENV-1 and DENV-2 serotypes in the nation's capital and largest city, Dhaka, suggesting the absence of DENV-3 and DENV-4 [29]. The epidemiology of dengue transmission in Bangladesh is therefore complex and fluid.…”

“…Several variable factors, such as sample collection time, storage and transportation, contribute to the differing sensitivity of tests performed under separate circumstances. Quite a few studies have highlighted the use of NS1 antigen and anti-NS1 antibody as a key dengue diagnostic device as it has the capacity to differentiate different serotypes and also helps to distinguish between primary and subsequent infections [27][28][29]. However, possibilities for improvement remain, with, for example, a reliable, cost-effective, highly specific and ultra-sensitive tool yet to be designed.…”

Knowledge, awareness and preventive practices of dengue outbreak in Bangladesh: a countrywide study

“…The incidence and transmission of dengue is in uenced by a variety of factors such as uncontrolled population growth, urbanization, deterioration in waste management systems, changes in the climate and lack of effective vector control [12]. Water storage practice as the water supplies are inadequate mainly in metropolitan cities is also regarded as a major contributor to dengue epidemics [13].…”