NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in<i>Arabidopsis</i>

Chen, Liang; Guan, Liping; Qian, Pingping; Xu, Fan; Z, Wu; Wu, Yujun; He, Kongwang; Gou, Xiaoping; Li, Jia; Hou, Suiwen

doi:10.1242/dev.129098

Cited by 24 publications

(30 citation statements)

References 69 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among the transcriptional co-regulators we found two significantly enriched transcriptional co-activators: MED16, which is part of the mediator complex that links TFs to RNA Pol II (Kidd et al 2011), and HAC1, which is a histone acetyl transferase (HAT) (Deng et al 2007). Combined with previous data showing a link between FAMA and RNA Pol II (Chen et al 2016), this suggests that FAMA activates genes both directly by recruiting RNA Pol II and by opening up the chromatin for other transcriptional regulators. Among transcriptional co-repressors were TOPLESS (TPL)-related proteins TPR3 and TPR4 and LEUNIG (LUG) and LEUNIG HOMOLOG (LUH), which recruit histone deacetylases (HDACs) to TFs (Long et al 2006).…”

Section: Figure 5: 'Fama Interactome' Experiments -Pl With Tid Revealssupporting

confidence: 70%

“…The low abundance of FAMA and FAMAexpressing cells renders identification of interaction partners and cell type-specific nuclear proteins by traditional methods challenging and makes it well suited for a proof-of-concept experiment. Potential FAMA interaction partners were previously identified in yeast-2-hybrid (Y2H) and bimolecular fluorescence complementation studies (Chen et al 2016;Kanaoka et al 2008;Lee, Lucas, and Sack 2014;Li, Yang, and Chen 2018;Matos et al 2014;Ohashi-Ito and Bergmann 2006), but only few have been confirmed by in vivo functional data.…”

Section: Testing Turboid's Potential To Identify Partners Of a Very Lmentioning

confidence: 99%

See 1 more Smart Citation

Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID

Mair

Branon

et al. 2019

Preprint

View full text Add to dashboard Cite

Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, or events. Proximity labeling enables discovery of protein neighborhoods defining functional complexes and/or organellar protein compositions. Recent technological improvements, namely two highly active biotin ligase variants (TurboID and miniTurboID), allowed us to address two challenging questions in plants: (1) what are in vivo partners of a low abundant key developmental transcription factor and (2) what is the nuclear proteome of a rare cell type? Proteins identified with FAMA-TurboID include known interactors of this stomatal transcription factor and novel proteins that could facilitate its activator and repressor functions. Directing TurboID to stomatal nuclei enabled purification of cell type-and subcellular compartment-specific proteins. Broad tests of TurboID and miniTurboID in Arabidopsis and N. benthamiana and versatile vectors enable customization by plant researchers.

show abstract

Section: Figure 5: 'Fama Interactome' Experiments -Pl With Tid Revealssupporting

confidence: 70%

Section: Testing Turboid's Potential To Identify Partners Of a Very Lmentioning

confidence: 99%

Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID

Mair

Branon

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Some of these marker genes could potentially be involved in regulating the development of stomatal lineage cells. SLAC1 and SCAP1(DOF5.7) play important roles in regulating the development of stomatal lineage cells (Engineer et al, 2015; Chen et al, 2016). Consistently, a high level of expression of SLAC1 and DOF5.7 was detected in YGC and GMC (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

Global Dynamic Molecular Profiles of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing

Liu

Zhou

Guo

et al. 2020

Preprint

View full text Add to dashboard Cite

30The regulation of stomatal lineage cell development has been extensively investigated. 31 However a comprehensive characterization of this biological process based on 32 single-cell transcriptome analysis has not yet been reported. Here, we performed 33 RNA-seq on over 12,844 individual cells from the cotyledons of five-day-old 34 Arabidopsis seedlings. We identified 11 cell clusters corresponding mostly to cells at 35 specific stomatal developmental stages with a series of new marker genes. 36 Comparative analysis of genes with the highest variable expression in these cell 37 clusters revealed three transcriptional networks that regulate the development of 38 mesophyll and guard cells, as well as the differentiation from protodermal to guard 39 mother cells. We investigated the developmental dynamics of marker genes via 40 pseudo-time analysis which revealed potential interactions between them. The 41 identification of several novel marker genes suggests new regulatory mechanisms 42 during development of stomatal cell lineage. 43 44 45 46 47 48 49 50 51 52 3 / 37 53 54 55 75 MacAlister et al., 2007; Pillitteri et al., 2007). To specify each cell state differentiation, 76 SPCH, MUTE, and FAMA form heterodimers with two paralogous bHLH-leucine 77 zipper (bHLH-LZ) transcription factors, SCREAM (SCRM) and SCRM2 (Kanaoka et 78 al., 2008). In addition, two partially redundant R2R3 MYB transcription factors, 79 FOUR LIPS (FLP) and MYB88, control stomatal terminal differentiation 80 independently of FAMA (GMC to GCs) (Lai et al., 2005; Ohashi-Ito and Bergmann, 81 2006). Two secreted cysteine-rich peptides, EPIDERMAL PATTERNING FACTOR1 82 4 / 37 (EPF1) and EPF2, are expressed at later and earlier stages of stomatal development, 83 respectively. These peptides are perceived by the cell-surface receptors, ERECTA 84 (ER)-family leucine-rich repeat receptor kinases (LRR-RKs), ER-LIKE1 (ERL1) and 85 ERL2, resulting in inhibition of stomatal development (Shpak et al., 2005; Hara et al., 86 2007; Hunt and Gray, 2009b; Lee et al., 2012). The receptor-like protein TOO 87 MANY MOUTHS (TMM) modulates the signaling strength of ER-family receptor 88kinases in a domain-specific manner (Nadeau and Sack, 2002; Lee et al., 2012). 89 Genetic evidence suggests that these signals are mediated via a mitogen-activated 90 protein kinase (MAPK) cascade, which eventually downregulates the transcription 91 factors responsible for initiating stomatal lineage via direct phosphorylation 92 (Bergmann et al., 2004; Lampard et al., 2008; Lampard et al., 2009; Kim et al., 2012). 93 Stomagen (also known as EPF-LIKE9) peptide promotes stomatal development by 94 competing with EPF2 for binding to ER (Sugano et al., 2010; Zhang et al., 2014; 95 Hronkova et al., 2015). One homeodomain-leucine zipper IV (HD-ZIP IV) protein, 96 HOMEODOMAIN GLABROUS2 (HDG2), acts as a key epidermal component 97 promoting stomatal differentiation (Peterson et al., 2013). It is highly expressed in 98 meristemoids, and a hdg2 mutant exhibits delayed meristemoid-to-GM...

show abstract

“…The GUS staining assay was performed as previously described (Chen et al, 2016) with T3 ProB1L:GUS transgenic plants of 1-day-old seedlings, 2-day-old seedlings, 10-day-old seedlings, and 8-week-old mature plants. After staining, the samples were rinsed with acetic acid/methanol [1:3 (v/v)].…”

Section: Methodsmentioning

confidence: 99%

BYPASS1-LIKE, A DUF793 Family Protein, Participates in Freezing Tolerance via the CBF Pathway in Arabidopsis

Chen

Zhang

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

The C-REPEAT BINDING FACTOR signaling pathway is strictly modulated by numerous factors and is essential in the cold response of plants. Here, we show that the DUF793 family gene BYPASS1-LIKE modulates freezing tolerance through the CBFs in Arabidopsis . The expression of B1L was rapidly induced under cold treatment. Comparing to wild type, B1L knockout mutants were more sensitive to freezing treatment, whereas B1L-overexpressing lines were more tolerant. The expression of CBF s and CBF target genes was significantly decreased in b1l mutant. Using yeast two-hybrid screening system, 14-3-3λ was identified as one of proteins interacting with B1L. The interaction was confirmed with bimolecular fluorescence complementation assay and co-immunoprecipitation assay. Biochemical assays revealed that b1l mutation promoted the degradation of CBF3 compared to wild type, whereas 14-3-3κλ mutant and b1l 14-3-3κλ mutant suppressed the degradation of CBF3. Consistently, 14-3-3κλ and b1l 14-3-3κλ mutants showed enhanced freezing tolerance compared to wild type. These results indicate that B1L enhances the freezing tolerance of plants, at least partly through stabilizing CBF. Our findings improve our understanding of the regulation of CBF in response to cold stress.

show abstract

NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation inArabidopsis

Cited by 24 publications

References 69 publications

Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID

Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID

Global Dynamic Molecular Profiles of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing

BYPASS1-LIKE, A DUF793 Family Protein, Participates in Freezing Tolerance via the CBF Pathway in Arabidopsis

Contact Info

Product

Resources

About