Novel synthesis of nanosilver particles using plant active principle aloin and evaluation of their cytotoxic effect against Staphylococcus aureus

Kumar, Thota Venkata Chaitanya; Prasad, Tollamadugu Naga Venkata Krishna Vara; Adilaxmamma, K; Alpharaj, Mekapogu; Yegireddy, Muralidhar; Prasad, P.

doi:10.1016/s2222-1808(14)60421-7

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…Among the compounds screened, ANS showed highest antibacterial activity against resistant bacteria followed by CNS and BNS. Similar observations of potent antibacterial activity of ANS against Staphylococcus aureus with an MIC of 21.8 ng/mL were reported (Kumar et al 2013(Kumar et al , 2014. Several authors evaluated the antibacterial activity of citrate mediated silver nanoparticles against E. coli O157:H7 and observed a MIC of 12.43 ppm; (Petrus et al 2011); E. coli (ATCC8739) with an MIC of 160 ppm (ElKheshen and El-Rab 2012).…”

Section: Minimum Inhibitory Concentrationsupporting

confidence: 67%

First report on rapid screening of nanomaterial-based antimicrobial agents against β-lactamase resistance using pGLO plasmid transformed Escherichia coli HB 101 K-12

Raj¹,

Yegireddy²,

Sravanthi³

et al. 2015

Appl Nanosci

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Combating antibiotic resistance requires discovery of novel antimicrobials effective against resistant bacteria. Herein, we present for the first time, pGLO plasmid transformed Escherichia coli HB 101 K 12 as novel model for screening of nanomaterial-based antimicrobial agents against b-lactamase resistance. E. coli HB 101 was transformed by pGLO plasmid in the presence of calcium chloride (50 mM; pH 6.1) aided by heat shock (0-42-0°C). The transformed bacteria were grown on Luria-Bertani agar containing ampicillin (amp) and arabinose (ara). The transformed culture was able to grow in the presence of ampicillin and also exhibited fluorescence under UV light. Both untransformed and transformed bacteria were used for screening citrate-mediated nanosilver (CNS), aloin-mediated nanosilver (ANS), 11-a-ketoboswellic acid (AKBA)-mediated nanosilver (BNS); nanozinc oxide, nanomanganese oxide (NMO) and phytochemicals such as aloin and AKBA. Minimum inhibitory concentrations (MIC) were obtained by microplate method using q-iodo nitro tetrazolium indicator. All the compounds were effective against transformed bacteria except NMO and AKBA. Transformed bacteria exhibited reverse cross resistance against aloin. ANS showed the highest antibacterial activity with a MIC of 0.32 ppm followed by BNS (10.32 ppm), CNS (20.64 ppm) and NZO (34.83 ppm). Thus, pGLO plasmid can be used to induce resistance against b-lactam antibiotics and the model can be used for rapid screening of new antibacterial agents effective against resistant bacteria.

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Section: Minimum Inhibitory Concentrationsupporting

confidence: 67%

First report on rapid screening of nanomaterial-based antimicrobial agents against β-lactamase resistance using pGLO plasmid transformed Escherichia coli HB 101 K-12

Raj¹,

Yegireddy²,

Sravanthi³

et al. 2015

Appl Nanosci

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“…Phytogenic NS synthesised using CE was found to be safer than chemically synthesised NS in cytotoxicity assay on mouse splenocytes. The safety of phytochemically synthesised NS using aloin over chemical mediated nano-silver was reported earlier [19,20]. On the other hand, chemically synthesised NS was reported to be toxic to HepG2, mice primary cell cultures and colon Caco2 cell linesat concentrations ranging from 1 to 20 µg/ml [60,61].…”

Section: Discussionmentioning

confidence: 80%

“…NS is reported to have potent antibacterial effect against many bacterial species [19][20][21]. A previous study reported that NS dressing protects from bacterial contamination in a variety of chronic non-healing wounds.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the wound healing efficacy of chemical and phytogenic silver nanoparticles

Pothireddy

Adilaxmamma

Mekapogu³

et al. 2016

IET nanobiotechnol.

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Wound healing requires a series of cellular events and a cascade of co-ordinated and systemic biochemical events. Silver nanoparticles possess many beneficial properties for wound management including antibacterial, anti-inflammatory and pro-healing properties. In this study, the authors investigated the wound healing properties of extract mediated nanosilver (CENS) particles in comparison with 1% povidone iodine, citrate mediate NS and CE treatments. The topical application of CENS showed good antibacterial activity and accelerated wound healing with complete epithelialisation and normal re-growth of hair in all three models of study: namely, excision, incision and dead space models in rats compared with all other treatments. CENS was also found to promote collagen synthesis, stabilise wound besides countering oxidative stress and stimulating cellular proliferation CENS could be a novel therapeutic agent for wound management.

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“…The inquiry on the pharmacological activity of diverse plants and plant products is growing considerably in the recent past. Novel applications of plants and plant products such as amelioration of heavy metal induced toxicities,[ 4 5 6 ] synthesis of nanoparticles[ 7 8 9 ] including normalization of dyslipidemias[ 10 11 12 ] have been reported. Chickpea or Bengal gram ( Cicer arientum L) is the second largest legume cultivated in the world providing a rich source of dietary protein.…”

Section: Introductionmentioning

confidence: 99%

Antihyperlipidemic activity of chickpea sprouts supplementation in ovariectomy-induced dyslipidemia in rats

Harini

Adilaxmamma²,

Mohan

et al. 2015

J Ayurveda Integr Med

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Background:Phytoestrogens are increasingly becoming popular as alternatives for hormone replacement therapy in postmenopausal condition.Objective:In this study, the antihyperlipidemic effect of chickpea (Cicer arientum) sprouts was evaluated in ovariectomy-induced dyslipidemia in rat model in comparison with standard antihyperlipidemic agent atorvastatin.Materials and Methods:A total of 24 female adult Wistar rats were divided into four groups that is, Group I - Control; Group II - Ovariectomized (OVX) rats; Group III - OVX + germinated chickpea sprouts (20% in diet) and Group IV OVX + atorvastatin (1.2 mg/kg b.wt, p.o.). Body and organ weights, serum, and liver lipid profile were assessed at the end of 8 weeks.Results:The results indicated that ovariectomy significantly (P < 0.05) increased total cholesterol, nonhigh-density lipoprotein cholesterol and triglycerides (TGs) in serum and liver. The total lipid and phospholipid content in liver were also significantly (P < 0.05) increased. The weights of uterus and heart were significantly (P < 0.05) decreased. Dietary supplementation with germinated chickpea normalized the lipid profile in serum and liver. Further, high-density lipoprotein (HDL) cholesterol, body weight, uterine, heart, and spleen weights were significantly (P < 0.05) increased. Atorvastatin administration showed similarly normalized lipid profile, but showed no improvement on decreased uterus and heart weights. Histopathological examination revealed fatty changes in liver, uterine atrophy, and subintimal fat accumulation in aorta in OVX group. The changes were mild in chickpea group with no improvement in statin group.Conclusions:Germinated seeds of chickpea showed significant antihyperlipidemic activity, which was comparable to atorvastatin. Further, germinated chickpea improved organ weights and helped in the reversal of histopathological changes suggesting its usefulness in postmenopausal condition.

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Novel synthesis of nanosilver particles using plant active principle aloin and evaluation of their cytotoxic effect against Staphylococcus aureus

Cited by 12 publications

References 19 publications

First report on rapid screening of nanomaterial-based antimicrobial agents against β-lactamase resistance using pGLO plasmid transformed Escherichia coli HB 101 K-12

First report on rapid screening of nanomaterial-based antimicrobial agents against β-lactamase resistance using pGLO plasmid transformed Escherichia coli HB 101 K-12

Evaluation of the wound healing efficacy of chemical and phytogenic silver nanoparticles

Antihyperlipidemic activity of chickpea sprouts supplementation in ovariectomy-induced dyslipidemia in rats

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