Novel Surfactants with Diglutamic Acid Polar Head Group: Drug Solubilization and Toxicity Studies

Ménard, Nathalie; Tsapis, Nicolas; Poirier, Cécile; Arnauld, Thomas; Moine, Laurence; Gignoux, C.; Lefoulon, François; Péan, Jean-Manuel

doi:10.1007/s11095-012-0714-8

Cited by 11 publications

(13 citation statements)

References 24 publications

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“…In the case of ANS, this follows literature examples of dye binding to and sensitization by both hydrophobic protein environments [26] and surfactant micelles [27]. With pyrene, a decreased I 1 / I 3 ratio is strongly indicative of decreased microenvironment polarity [28] and such a photophysical change is consistent with the known preferential partitioning of pyrene into a hydrophobic micelle core [29].…”

Section: Resultssupporting

confidence: 77%

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Nanoparticle self‐assembly by a highly stable recombinant spider wrapping silk protein subunit

Tremblay

Orrell

et al. 2013

FEBS Letters

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a b s t r a c tArtificial spider silk proteins may form fibers with exceptional strength and elasticity. Wrapping silk, or aciniform silk, is the toughest of the spider silks, and has a very different protein composition than other spider silks. Here, we present the characterization of an aciniform protein (AcSp1) subunit named W 1 , consisting of one AcSp1 199 residue repeat unit from Argiope trifasciata. The structural integrity of recombinant W 1 is demonstrated in a variety of buffer conditions and time points. Furthermore, we show that W 1 has a high thermal stability with reversible denaturation at $71°C and forms self-assembled nanoparticle in near-physiological conditions. W 1 therefore represents a highly stable and structurally robust module for protein-based nanoparticle formation.

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Section: Resultssupporting

confidence: 77%

“…Both fluorophores indicate behavior consistent with a critical concentration for W 1 nanoparticle formation, akin to a critical micelle concentration (cmc). This can be most reliably estimated from the pyrene I 1 /I 3 inflection point [29], providing a cmc estimate of $20 lM (Fig. 6D).…”

Section: Self-assembly Of W 1 Protein Into Nanoparticlesmentioning

confidence: 99%

Nanoparticle self‐assembly by a highly stable recombinant spider wrapping silk protein subunit

Tremblay

Orrell

et al. 2013

FEBS Letters

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“…Dioctyl sodium sulfosuccinate (DOSS), one of the chemical components of CE [ 60 ], has been documented to cause mass detachment of the intestinal epithelium of horses and guinea pigs at concentrations ranging from 0.5 g/kg to 1.0 g/kg of body weight [ 61 ]. Similarly, Polysorbate 80, another component of CE, commonly used as an emulsifier in food products, has been linked to plasma membrane damage in human umbilical vein endothelial cells in-vitro [ 62 ]. Therefore, the injury induced by CE may be attributed to one or both components.…”

Section: Discussionmentioning

confidence: 99%

Heme Oxygenase-1 Protects Corexit 9500A-Induced Respiratory Epithelial Injury across Species

Duggal

Oliva

et al. 2015

PLoS ONE

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The effects of Corexit 9500A (CE) on respiratory epithelial surfaces of terrestrial mammals and marine animals are largely unknown. This study investigated the role of CE-induced heme oxygenase-1 (HO-1), a cytoprotective enzyme with anti-apoptotic and antioxidant activity, in human bronchial airway epithelium and the gills of exposed aquatic animals. We evaluated CE-mediated alterations in human airway epithelial cells, mice lungs and gills from zebrafish and blue crabs. Our results demonstrated that CE induced an increase in gill epithelial edema and human epithelial monolayer permeability, suggesting an acute injury caused by CE exposure. CE induced the expression of HO-1 as well as C-reactive protein (CRP) and NADPH oxidase 4 (NOX4), which are associated with ROS production. Importantly, CE induced caspase-3 activation and subsequent apoptosis of epithelial cells. The expression of the intercellular junctional proteins, such as tight junction proteins occludin, zonula occludens (ZO-1), ZO-2 and adherens junctional proteins E-cadherin and Focal Adhesion Kinase (FAK), were remarkably inhibited by CE, suggesting that these proteins are involved in CE-induced increased permeability and subsequent apoptosis. The cytoskeletal protein F-actin was also disrupted by CE. Treatment with carbon monoxide releasing molecule-2 (CORM-2) significantly inhibited CE-induced ROS production, while the addition of HO-1 inhibitor, significantly increased CE-induced ROS production and apoptosis, suggesting a protective role of HO-1 or its reaction product, CO, in CE-induced apoptosis. Using HO-1 knockout mice, we further demonstrated that HO-1 protected against CE-induced inflammation and cellular apoptosis and corrected CE-mediated inhibition of E-cadherin and FAK. These observations suggest that CE activates CRP and NOX4-mediated ROS production, alters permeability by inhibition of junctional proteins, and leads to caspase-3 dependent apoptosis of epithelial cells, while HO-1 and its reaction products protect against oxidative stress and apoptosis.

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“…In contrast, Au NPs were generally shown to be non‐toxic or only slightly toxic (viability of HUVECs larger than 80% after NP exposure) at relatively high concentrations (Bartczak et al, ; Soenen et al, ; Li et al, ; Klingberg et al, ). The micelles based on polymers also showed little to no toxicity to HUVECs (Menard et al, ; Su et al, ; Liu et al, ).…”

Section: Toxic Effects Of Nps To Huvecsmentioning

confidence: 99%

The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review

Cao

Gong

Liu

et al. 2017

J of Applied Toxicology

230

173

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With the rapid development of nanotechnologies, nanoparticles (NPs) are increasingly produced and used in many commercial products, which could lead to the contact of human blood vessels with NPs. Thus, it is necessary to understand the adverse effects of NPs to relevant cells lining human blood vessels, especially endothelial cells (ECs) that cover the lumen of blood vessels. Human umbilical vein endothelial cells (HUVECs) are among one of the most popular models used for ECs in vitro. In the present review, we discussed studies that have used HUVECs as a model to investigate the EC-NP interactions, the toxic effects of NPs on ECs and the mechanisms. The results of these studies indicated that NPs could be internalized into HUVECs by the endocytosis pathway as well as transported across HUVECs by exocytosis and paracellular pathways. Exposure of HUVECs to NPs could induce cytotoxicity, genotoxicity, eNOS uncoupling and endothelial activation, which could be explained by NP-induced oxidative stress, inflammatory response and dysfunction of organelles. In addition, some studies have also evaluated the influences of microenvironment (e.g. the presence of proteins and excessive nutrients), the physiological and/or pathological stimuli related to the diversity of ECs (e.g. shear stress, cyclic stretch and inflammatory stimuli), and the physicochemical properties of NPs on the responses of ECs to NP exposure. In conclusion, it has been suggested that HUVECs could be considered as a relatively reliable and simple in vitro model for ECs to predict and evaluate the toxicity of NPs to endothelium. Copyright © 2017 John Wiley & Sons, Ltd.

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Novel Surfactants with Diglutamic Acid Polar Head Group: Drug Solubilization and Toxicity Studies

Abstract: Among the surfactants tested, stearoyl-DG is the more efficient for drug solubilization but its use is limited by its possible liver toxicity.

Cited by 11 publications

References 24 publications

Nanoparticle self‐assembly by a highly stable recombinant spider wrapping silk protein subunit

Nanoparticle self‐assembly by a highly stable recombinant spider wrapping silk protein subunit

Heme Oxygenase-1 Protects Corexit 9500A-Induced Respiratory Epithelial Injury across Species

The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review

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