Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.12.074 View full text
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Line Naomi Lund, Per-Erik Gustavsson, Roice Michael, Johan Lindgren, Leif Nørskov-Lauritsen, Martin Lund, Gunnar Houen, Arne Staby, Phaedria M. St. Hilaire

Abstract: Small synthetic ligands for protein purification have become increasingly interesting with the growing need for cheap chromatographic materials for protein purification and especially for the purification of monoclonal antibodies (mAbs). Today, Protein A-based chromatographic resins are the most commonly used capture step in mAb down stream processing; however, the use of Protein A chromatography is less attractive due to toxic ligand leakage as well as high cost. Whether used as an alternative to the Protein …

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