Novel mass spectrometry imaging software assisting labeled normalization and quantitation of drugs and neuropeptides directly in tissue sections

Källback, Patrik; Shariatgorji, Mohammadreza; Nilsson, Anna; Andrén, Per E.

doi:10.1016/j.jprot.2012.07.034

Cited by 139 publications

(147 citation statements)

References 24 publications

(24 reference statements)

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“…This finding is well in line with previous reports showing expression of the Tac1 gene in human and rat brain and human spleen (6,14). Moreover, peptide expression studies have detected SP in the brain using mass spectrometry (26,27). Spectra obtained from our study showed that SP was detected with high abundance in both tissues; however post-translational modifications were not identified on SP.…”

Section: Discussionsupporting

confidence: 92%

Mouse Hemokinin-1 Decapeptide Subjected to a Brain-specific Post-translational Modification

Deliconstantinos

Barton

Soloviev

et al. 2018

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Abstract. Background Tachykinins as a vast family of highly conserved peptides share the signature C-terminal motif FXGLM-NH2 (where X in mammals is F, Y, V; in fish, H, I, and in ascidians T) (1-3). The tachykinin mouse hemokinin-1 (mHK-1) discovered as an autocrine factor during pro-B lymphocyte differentiation introduced the group of endokinins (4) which also comprises human HK-1 (5), and its extended forms endokinin A (EKA) and endokinin (EKB) (6) or truncated forms such as the tachykinin human hemokinin (hHK-1) (4-11) (7). Tachykinins act as agonists on the three mammalian tachykinin receptors neurokinin 1, 2, 3 (NK1, NK2, NK3) with different potencies (5, 8). The hydrophobic C-terminal tachykinin motif activates the receptor, and the C-terminal amidation is crucial for activation (9, 10). Binding studies on the NK1 receptor showed mHK-1 as having similar binding affinity on this receptor as substance P (SP) (11-13) and a similar affinity for each of the mouse, rat and human NK1 receptors (5,7,14).The opposite effects of mHK-1 and SP in thermal hyperalgesia, have suggested that the two peptides could bind to different subtypes of the NK1 receptor (15, 16). The activation of NK1 receptor is related to the release of endogenous proopiomelanocortin, as well as the increased expression levels of the μ-opioid receptor in analgesia (17). Moreover, it was reported that the N-and C-terminal fragments of the mHK-1 elicited opposite biological effects (i.e. inhibition or induction of pruritus) when administered in rats, through the activation of the NK1 receptor (18). Recent evidence showed anxiolytic and anti-depressant-like actions for mHK-1 (19). The nociceptive behaviors induced by mHK-1 were inhibited by NMDA receptor antagonists. A significant increase in glutamate levels was observed, which was reduced by co-administration with NMDA receptor antagonists, suggesting that mHK-1-induced nociceptive effects may be mediated by the NMDA receptor (20). The Tachykinin receptor-1 (TACR1) gene was found among the neurotoxicityassociated genes in brain tissue that were significantly modulated after irradiation, suggesting that catenin-β1 and tumor suppressor-related transcription regulation are significantly activated by radiation and/or minocycline, suggesting minocycline as a potential neuroprotectant against radiation-induced damage (21). Moreover, the TACR3 was shown to be highly expressed in oral squamous cell carcinoma suggesting TAC3 was released by the peripheral sensory nerves which may act in tumor cells and that TACR3 signaling may, in turn, contribute to tumor progression (22).

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Section: Discussionsupporting

confidence: 92%

Mouse Hemokinin-1 Decapeptide Subjected to a Brain-specific Post-translational Modification

Deliconstantinos

Barton

Soloviev

et al. 2018

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“…Källback et al recently developed an MSI software for the quantification of drugs and neuropeptides in tissue sections [23]. Among the different normalization methods, the use of labeled internal standards (ISTDs) showed the best linear regression fit [24,25].…”

Section: Introductionmentioning

confidence: 99%

Quantification in MALDI-MS imaging: what can we learn from MALDI-selected reaction monitoring and what can we expect for imaging?

et al. 2014

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Quantification by mass spectrometry imaging (Q-MSI) is one of the hottest topics of the current discussions among the experts of the MS imaging community. If MSI is established as a powerful qualitative tool in drug and biomarker discovery, its reliability for absolute and accurate quantification (QUAN) is still controversial. Indeed, Q-MSI has to deal with several fundamental aspects that are difficult to control, and to account for absolute quantification. The first objective of this manuscript is to review the state-of-the-art of Q-MSI and the current strategies developed for absolute quantification by direct surface sampling from tissue sections. This includes comments on the quest for the perfect matrix-matched standards and signal normalization approaches. Furthermore, this work investigates quantification at a pixel level to determine how many pixels must be considered for accurate quantification by ultraviolet matrix-assisted laser desorption/ionization (MALDI), the most widely used technique for MSI. Particularly, this study focuses on the MALDI-selected reaction monitoring (SRM) in rastering mode, previously demonstrated as a quantitative and robust approach for small analyte and peptide-targeted analyses. The importance of designing experiments of good quality and the use of a labeled compound for signal normalization is emphasized to minimize the signal variability. This is exemplified by measuring the signal for cocaine and a tryptic peptide (i.e., obtained after digestion of a monoclonal antibody) upon different experimental conditions, such as sample stage velocity, laser power and frequency, or distance between two raster lines. Our findings show that accurate quantification cannot be performed on a single pixel but requires averaging of at least 4-5 pixels. The present work demonstrates that MALDI-SRM/MSI is quantitative with precision better than 10-15 %, which meets the requirements of most guidelines (i.e., in bioanalysis or toxicology) for quantification of drugs or peptides from tissue homogenates.

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“…Mouse brain tissue treated with one of three different drug compounds (cimetidine, imipramine or "compound c") had their sections washed with ammonium acetate buffer at pH 10, significantly increasing signal intensities as compared to the results from using acidic or neutral buffers. 18) For the analysis of lipids, washing steps are usually avoided. However, Angel et al developed a washing protocol for the analysis of lipids in negative mode utilizing ammonium formate at pH 6.4 or ammonium acetate at pH 6.7, which significantly increased the number of detectable analytes along with their signal intensities.…”

Section: Washingmentioning

confidence: 99%

“…Recently, the Andrén group presented a quantification method that is useful for the analysis of a pre-selected target molecule. 18) Standard solutions of the target compound are spotted at known concentrations onto control tissue. The spots (5-8 in total) then serve as references for a calibration curve.…”

Section: Quanti Cation and Data Normalizationmentioning

confidence: 99%

MALDI Imaging Mass Spectrometry—A Mini Review of Methods and Recent Developments

et al. 2013

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As the only imaging method available, Imaging Mass Spectrometry (IMS) can determine both the identity and the distribution of hundreds of molecules on tissue sections, all in one single run. IMS is becoming an established research technology, and due to recent technical and methodological improvements the interest in this technology is increasing steadily and within a wide range of scientific fields. Of the different IMS methods available, matrix-assisted laser desorption/ionization (MALDI) IMS is the most commonly employed. The course at IMSC 2012 in Kyoto covered the fundamental principles and techniques of MALDI-IMS, assuming no previous experience in IMS. This mini review summarizes the content of the one-day course and describes some of the most recent work performed within this research field.

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Novel mass spectrometry imaging software assisting labeled normalization and quantitation of drugs and neuropeptides directly in tissue sections

Cited by 139 publications

References 24 publications

Mouse Hemokinin-1 Decapeptide Subjected to a Brain-specific Post-translational Modification

Mouse Hemokinin-1 Decapeptide Subjected to a Brain-specific Post-translational Modification

Quantification in MALDI-MS imaging: what can we learn from MALDI-selected reaction monitoring and what can we expect for imaging?

MALDI Imaging Mass Spectrometry—A Mini Review of Methods and Recent Developments

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