Novel liposome-based and in situ gelling artificial tear formulation for dry eye disease treatment

Acar, Duygu Besnili; Molina‐Martínez, Irene T.; Gómez-Ballesteros, Miguel; Guzmán-Navarro, Manuel; Benítez-del-Castillo, J.M.; Herrero–Vanrell, R.

doi:10.1016/j.clae.2017.11.004

Cited by 43 publications

(20 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cationic and anionic liposomes were prepared by using the lipid film hydration method [20,21] with diverse modifications depending on the used lipids. In order to prepare lipid bilayers, lipids were first dissolved in chloroform.…”

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes

et al. 2019

View full text Add to dashboard Cite

Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium. Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes with phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can be used in bacterial infections against Escherichia coli with high inhibitory activity.

show abstract

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Liposomes were prepared by using the lipid thin-film hydration method [39,40]. First, appropriate quantities of DOPE and ruthenium-based lipids were dissolved in 2 mL chloroform.…”

Section: Liposome Preparationmentioning

confidence: 99%

Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material

et al. 2020

View full text Add to dashboard Cite

Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+ complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between Kapp and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide’s condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.

show abstract

“…They contribute to forming a viscous continuous layer on the ocular surface able to partially protect the drug against lacrimal drainage, extending the ocular residence time [29,30]. In fact, polymers such as carboxymethylcellulose, hydroxypropyl methylcellulose (HPMC), hyaluronic acid or xanthan gum have been employed with this aim [19,30,31]. All of them have shown also good in vitro and in vivo tolerance.…”

Section: Introductionmentioning

confidence: 99%

Osmoprotectants in Hybrid Liposome/HPMC Systems as Potential Glaucoma Treatment

Gómez-Ballesteros¹,

López-Cano²,

Bravo‐Osuna

et al. 2019

Polymers

Self Cite

View full text Add to dashboard Cite

The combination of acetazolamide-loaded nano-liposomes and Hydroxypropyl methylcellulose (HPMC) with similar components to the preocular tear film in an osmoprotectant media (trehalose and erythritol) is proposed as a novel strategy to increase the ocular bioavailability of poorly soluble drugs. Ophthalmic formulations based on acetazolamide-loaded liposomes, dispersed in the osmoprotectant solution (ACZ-LP) or in combination with HPMC (ACZ-LP-P) were characterized and in vivo evaluated. The pH and tonicity of both formulations resulted in physiological ranges. The inclusion of HPMC produced an increment in viscosity (from 0.9 to 4.7 mPa·s. 64.9 ± 2.6% of acetazolamide initially included in the formulation was retained in vesicles. In both formulations, a similar onset time (1 h) and effective time periods were observed (7 h) after a single instillation (25 μL) in normotensive rabbits’ eyes. The AUC0–8h of the ACZ-LP-P was 1.5-fold higher than of ACZ-LP (p < 0.001) and the maximum hypotensive effect resulted in 1.4-fold higher (p < 0.001). In addition, the formulation of ACZ in the hybrid liposome/HPMC system produced a 30.25-folds total increment in ocular bioavailability, compared with the drug solution. Excellent tolerance in rabbits’ eyes was confirmed during the study.

show abstract

Novel liposome-based and in situ gelling artificial tear formulation for dry eye disease treatment

Abstract: The liposome-based and in situ gelling artificial tear formulation presented good tolerance and suitable properties for topical ophthalmic administration. It may be beneficial in the treatment of dry eye disease.

Cited by 43 publications

References 23 publications

Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes

Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes

Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material

Osmoprotectants in Hybrid Liposome/HPMC Systems as Potential Glaucoma Treatment

Contact Info

Product

Resources

About