Novel Flavivirus or New Lineage of West Nile Virus, Central Europe

Bakonyi, Tamás; Hubálek, Zdeněk; Rudolf, Ivo; Nowotny, Norbert

doi:10.3201/eid1102.041028

Cited by 210 publications

(171 citation statements)

References 20 publications

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“…In relation to population abundance, the following species do not create a large nuisance problem in the Osijek area, but their roles in pathogen transmission has been confirmed by many authors (Lundström 1999;Gaunt et al 2001;Bakonyi et al 2005;Gratz 2006;Hubálek 2008). …”

Section: Disscussionmentioning

confidence: 91%

Seasonal dynamics of mosquitoes (Diptera: Culicidae) in Osijek (Croatia) for the period 1995–2004

et al. 2009

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The ten year research (1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004) of adult mosquitoes in the area of Osijek by dry ice baited CDC traps has provided significant information on mosquito species, species prevalence, their dynamics and seasonal activity. A total of 207,136 adult mosquitoes comprising twenty species belonging to the following genera: Anopheles, Ochlerotatus, Aedes, Culex, Coquillettidia, Culiseta and Uranotaenia were collected. The seven most common species collected during this study were: Aedes vexans (75.6%), Ochlerotatus sticticus (13.3%), Culex pipiens complex (5.9%), Anopheles maculipennis complex (1.9%), Oc. cantans (0.9%), Oc. caspius (0.6%) and Oc. excrucians (0.6%). The temporal changes in the relative abundance of the twenty mosquito species in the Osijek area were reflected in three peaks: in May, July and September. Seasonal population dynamics differed between years and between mosquito species in relation to wetland, urban and forest habitats and was influenced by water level of the Drava and the Danube rivers.

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Section: Disscussionmentioning

confidence: 91%

Seasonal dynamics of mosquitoes (Diptera: Culicidae) in Osijek (Croatia) for the period 1995–2004

et al. 2009

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“…This region frequently experiences calamity incidences of mosquitoes, and agents of human disease, including West Nile Virus (WNV), have been isolated from these insects [12,13]. The Sedlec site (48°47'N, 16°43'E, 176 m a.s.l.)…”

Section: Sitesmentioning

confidence: 99%

“…Great attention has been devoted to researching mosquito outbreaks, which occur relatively frequently in this area [1,2], while faunistic works are also numerous [3][4][5][6][7][8][9]. Especially significant are studies focusing on mosquitoes as potential vectors of pathogenic agents, particularly viruses [10][11][12][13][14][15]. However, in Moravia, as in the rest of Europe, research on seasonal dynamics of the most prominent mosquito species and their daily activity has been largely neglected.…”

Section: Introductionmentioning

confidence: 99%

Daily and seasonal variation in the activity of potential vector mosquitoes

Šebesta¹,

Gelbič

Peško

2011

Open Life Sciences

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In the course of season 2010 (May–September), three 2-day trappings of female mosquitoes were carried out at two sites in order to determine the daily activity of the common mosquito species (e.g. species from genus Culex, Aedes, Ochlerotatus, Anopheles, etc.) in the area. CDC light traps filled with CO2 and placed at a height of 1 m were used to trap individuals, and were sampled every 2 h. A total of 19,604 female mosquitoes from 20 identifiable species were trapped: 7,549 at Sedlec and 12,055 at Kančí obora. The activity of the major species of mosquitoes in South Moravia differed throughout the course of the day. Calamity species of the genus Aedes and Ochlerotatus remained active throughout the day and night, but with different course. Aedes vexans, Ae. cinereus, and Ae. rossicus were most active in the late afternoon and highly active for most of the night. Ochlerotatus sticticus was captured most often in the afternoon, and its abundance decreased rapidly before sunset. The activity of Oc. cantans s.l. (Oc. cantans + Oc. annulipes) females varied little during the day and night. The daily activity for the main vectors of West Nile virus, Culex pipiens and Cx. modestus, were totally different from that of other species. Cx. pipiens females showed significant night activity, while Cx. modestus was most active in the evening. Nighttime activity was also observed in female mosquitoes of the genus Anopheles.

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“…?show "fnote_aff1"$^! "content-markup(./author-grp [1]/aff|./author-grp [1]/dept-list)> West Nile virus (WNV; family Flaviviridae, genus Flavivirus) was first detected in a woman in the West Nile District of Uganda in 1937. 24 West Nile virus is an arthropod-borne virus and grouped in the Japanese encephalitis virus (JEV) serocomplex, which includes the St. Louis encephalitis virus, JEV, and Murray Valley encephalitis virus (MVEV), among others.…”

mentioning

confidence: 99%

“…Threshold cycle (Ct) values in at least 3 replicates are plotted against synthetic RNA copies on a log scale. The regression function and correlation coefficient (R 2 ) of qRT-PCR with probe of assay 1 [1] and synthetic control probe [2] are inserted into the plot. C, The limit of detection (LOD) was determined by using quantified serial dilutions in at least 3 replicates of external calibrator RNA, which sets the end-point limit of detection for 3 copies per reaction.…”

mentioning

confidence: 99%

Two New Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction Assays with Unique Target Sites for the Specific and Sensitive Detection of Lineages 1 and 2 West Nile Virus Strains

et al. 2010

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Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strainsMartin Eiden, Ariel Vina-Rodriguez, Bernd Hoffmann, Ute Ziegler, Martin H. Groschup 1Abstract. Two novel 1-step real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for the simultaneous detection of West Nile virus (WNV) lineage 1 and 2 strains were developed. Primers and the probe of assay 1 target the 59-untranslated region (UTR), whereas the amplicon of assay 2 is located in the nonstructural region NS2A, which enables an unambiguous and independent WNV diagnosis based on 2 different amplicons. Both assays allow the detection of as few as 2-4 genome copies of WNV strains NY99, Uganda B956, Kunjin, and Sarafend (all cultured on Vero cells). A new synthetic RNA mutant of the 59-UTR amplicon, which contains 6 twist inverted base-pair changes at the probe attachment site, was used as external calibrator control.

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Novel Flavivirus or New Lineage of West Nile Virus, Central Europe

Abstract: Rabensburg virus, isolated from Culex pipiens mosquitoes in central Europe, represents a new lineage of West Nile virus or a novel flavivirus of the Japanese encephalitis virus group.

Cited by 210 publications

References 20 publications

Seasonal dynamics of mosquitoes (Diptera: Culicidae) in Osijek (Croatia) for the period 1995–2004

Seasonal dynamics of mosquitoes (Diptera: Culicidae) in Osijek (Croatia) for the period 1995–2004

Daily and seasonal variation in the activity of potential vector mosquitoes

Two New Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction Assays with Unique Target Sites for the Specific and Sensitive Detection of Lineages 1 and 2 West Nile Virus Strains

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