Novel fabrication development for the application of polycaprolactone and composite polycaprolactone/hydroxyapitite scaffolds for bone tissue engineering

Shor, Lauren

doi:10.17918/etd-2966

Cited by 5 publications

(12 citation statements)

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“…COMSOL's microfluidic module is widely used to study microfluidic devices. The flow of a fluid through a microfluidic channel can be characterized by Reynolds number using equation (1):…”

Section: Computational Analysismentioning

confidence: 99%

“…Microelectromechanical systems (MEMS) were popularized with the introduction of the integrated circuit (IC). Since then, MEMS technologies have been utilized in many fields with great success [1][2][3][4][5]. Micro-molding, etching, and deposition of features on or in materials are frequently referred to as microfabrication [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…A combination of several tissue engineering and microfabrication techniques was utilized to develop the sensor presented in this paper, namely; (1) a digital micro-mirror device (DMD), (2) a freeform micro-plasma system, and (3) a multi-nozzle biological deposition system. (1) The DMD is a digital light processing unit that projects images of '.jpeg', '.bitmap', or '.gif' formats. The micro-mirrors have the option to switch between masks within a matter of microseconds, while offering high resolutions performance in spatial light modulation.…”

Section: Introductionmentioning

confidence: 99%

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A three-dimensional cell-laden microfluidic chip for in vitro drug metabolism detection

Hamid¹,

Wang²,

Zhao³

et al. 2014

Biofabrication

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Three-dimensional tissue platforms are rapidly becoming the method of choice for quantification of the heterogeneity of cell populations for many diagnostic and drug therapy applications. Microfluidic sensors and the integration of sensors with microfluidic systems are often described as miniature versions of their macro-scale counterparts. This technology presents unique advantages for handling costly and difficult-to-obtain samples and reagents as a typical system requires between 100 nL to 10 µL of working fluid. The fabrication of a fully functional cell-based biosensor utilizes both biological patterning and microfabrication techniques. A digital micro-mirror (photolithographic) system is initiated to construct the tissue platform while a cell printer is used to precisely embed the cells within the construct. Tissue construct developed with these technologies will provide an early diagnostic of a drug's potential use. A three-dimensional interconnected microfluidic environment has the potential to eliminate the limitations of the traditional mainstays of two-dimensional investigations. This paper illustrates an economical and an innovative approach of fabricating a three-dimensional cell-laden microfluidic chip for detecting drug metabolism.

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“…COMSOL's microfluidic module is widely used to study microfluidic devices. The flow of a fluid through a microfluidic channel can be characterized by Reynolds number using equation (1):…”

Section: Computational Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A three-dimensional cell-laden microfluidic chip for in vitro drug metabolism detection

Hamid¹,

Wang²,

Zhao³

et al. 2014

Biofabrication

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“…Moreover, they have relatively good mechanical strength [7]. In particular, PCL has relatively good elongation at break and high flexibility, and as an FDA-approved material, it is a promising material for clinical usage [2,8,9]. However, inserting a membrane or scaffold based on PCL into the body may cause an inflammatory reaction or infection caused by bacteria [10].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Nanostructured Polycaprolactone (PCL) Film Using a Thermal Imprinting Technique and Assessment of Antibacterial Function for Its Application

et al. 2022

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In the use of the medical devices, it is essential to prevent the attachment of bacteria to the device surface or to kill the attached bacteria. To kill bacteria, many researchers have used antibiotics or studied nanostructure-based antibacterial surfaces, which rely on mechanical antibacterial methods. Several polymers are widely used for device fabrication, one of which is polycaprolactone (PCL). PCL is biocompatible, biodegradable, easy to fabricate using 3D printing, relatively inexpensive and its quality is easily controlled; therefore, there are various approaches to its use in bio-applications. In addition, it is an FDA-approved material, so it is often used as an implantable material in the human body. However, PCL has no inherent antibacterial function, so it is necessary to develop antibacterial functions in scaffold or film-based PCL medical devices. In this study, process parameters for nanopillar fabrication were established through a simple thermal imprinting method with PCL. Finally, a PCL film with a flexible and transparent nanopillar structure was produced, and the mechano-bactericidal potential was demonstrated using only one PCL material. PCL with nanopillars showed bactericidal ability against Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) bacteria cultured on its surface that resulted in membrane damage and death due to contact with nanopillars. Additionally, bacteriostatic results were shown to inhibit bacterial growth and activity of Staphylococcus aureus (S. aureus) on PCL nanostructured columns. The fabricated nanopillar structure has confirmed that mechanically induced antibacterial function and can be applied to implantable medical devices.

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“…The field of biofabrication brings together the multidisciplinary field of engineering and integrated sciences to fabricate three-dimensional constructs that aid the generation or regeneration of organic tissues and organs [1,2]. The means by which this can be done is determined by many factors.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of three-dimensional scaffolds using precision extrusion deposition with an assisted cooling device

et al. 2011

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In the field of biofabrication, tissue engineering and regenerative medicine, there are many methodologies to fabricate a building block (scaffold) which is unique to the target tissue or organ that facilitates cell growth, attachment, proliferation and/or differentiation. Currently, there are many techniques that fabricate three-dimensional scaffolds; however, there are advantages, limitations and specific tissue focuses of each fabrication technique. The focus of this initiative is to utilize an existing technique and expand the library of biomaterials which can be utilized to fabricate three-dimensional scaffolds rather than focusing on a new fabrication technique. An expanded library of biomaterials will enable the precision extrusion deposition (PED) device to construct three-dimensional scaffolds with enhanced biological, chemical and mechanical cues that will benefit tissue generation. Computer-aided motion and extrusion drive the PED to precisely fabricate micro-scaled scaffolds with biologically inspired, porosity, interconnectivity and internal and external architectures. The high printing resolution, precision and controllability of the PED allow for closer mimicry of tissues and organs. The PED expands its library of biopolymers by introducing an assisting cooling (AC) device which increases the working extrusion temperature from 120 to 250 °C. This paper investigates the PED with the integrated AC's capabilities to fabricate three-dimensional scaffolds that support cell growth, attachment and proliferation. Studies carried out in this paper utilized a biopolymer whose melting point is established to be 200 °C. This polymer was selected to illustrate the newly developed device's ability to fabricate three-dimensional scaffolds from a new library of biopolymers. Three-dimensional scaffolds fabricated with the integrated AC device should illustrate structural integrity and ability to support cell attachment and proliferation.

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Novel fabrication development for the application of polycaprolactone and composite polycaprolactone/hydroxyapitite scaffolds for bone tissue engineering

Cited by 5 publications

References 0 publications

A three-dimensional cell-laden microfluidic chip for in vitro drug metabolism detection

A three-dimensional cell-laden microfluidic chip for in vitro drug metabolism detection

Fabrication of Nanostructured Polycaprolactone (PCL) Film Using a Thermal Imprinting Technique and Assessment of Antibacterial Function for Its Application

Fabrication of three-dimensional scaffolds using precision extrusion deposition with an assisted cooling device

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