Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs)

Abstract

A and Reddy, Subrayal M (2007) Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs). Journal of Materials Science, 42 (22) AbstractWe report the transmission electron microscopy (TEM) imaging of a hydrogel-based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that clearly displays molecularly imprinted cavities, ranging from 5-50 nm in size, that correlate (in terms of size)with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase.

The preparation of poly(2-hydroxyethyl methacrylate) and poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} by photoinitiated polymerisation-induced phase separation in water

“…As discussed above, for PHEMA homopolymers there was no apparent difference between samples dried by critical point drying or freeze drying. Compared to critical point drying, however, freeze drying is generally considered an inferior method of dehydration due to surface tension effects leading to specimen collapse [31,32]. Table 3).…”

Molecularly Imprinted Ligand-Free Nanogels for Recognizing Bee Venom-Originated Phospholipase A2 Enzyme

“…These micrographs, probably the first of their kind, show how the PLA2 enzyme binds to the nanogel matrix and, subsequently, leaves marks of its interaction by creating molecularly imprinted cavities ( Figure 3 f,i). TEM micrographs of the MIP-LFNGs after the PLA2 extraction also showed the presence of multiple spherical zones with different electron densities that sustain the latter affirmation [ 51 ]. Therefore, this structural detail is proof of the non-covalent interactions between the template and macromers and can be suggestive and characteristic of the presence of imprinted free nanocavities on the surface of synthesized MIP-LFNGs [ 52 ].…”

Effect of meat enhancement solutions with hydroxypropyl methylcellulose and konjac flour on texture and quality attributes of pale, soft, and exudative pork

“…Samples were transferred to the Texas A&M University Microscopy and Imaging Center for imaging preparation. Samples were excised to 2–3 mm cubes and prepared according to the methods of Ellis and Pendleton () and Hawkins, Ellis, Stevenson, Holxenburg, and Reddy (). Imaging was performed on a Joel JSM‐6400 equipped with a PGT EDS System.…”