Novel chemiluminescence-inducing cocktails, part II: Measurement of the anti-oxidant capacity of vitamins, thiols, body fluids, alcoholic beverages and edible oils

Ginsburg, Isaac; Sadovnic, Milu; Oron, Miriam; Kohen, Ron

doi:10.1163/1568560043696218

Cited by 35 publications

(31 citation statements)

References 9 publications

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“…Using a new chemiluminescence assay and an assay involving the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) (which are adapted to quantify antioxidants in whole blood), 1 we have shown that erythrocytes, platelets, and lymphocytes not only rapidly scavenge reactive oxygen species but also avidly bind to their surfaces a large variety of antioxidant polyphenols, which further enhance their oxidantscavenging abilities. 2,3 Such complexes could further act synergistically with low-molecular-weight antioxidants in plasma and with albumin to decompose reactive oxygen species.…”

Section: Quantifying Oxidant-scavenging Ability Of Bloodmentioning

confidence: 99%

Quantifying Oxidant-Scavenging Ability of Blood

Ginsburg¹,

Kohen²,

Koren³

2011

N Engl J Med

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To the Editor:We applaud the publication of the report regarding a 65-year-old woman who underwent an incorrect operation on the left hand, and we commend Ring et al. (Nov. 11 issue) 1 for making the case public. We agree with the discussion noting that the patient's limited English proficiency contributed to this error. Limited English proficiency has been associated with medication errors 2 and with a lack of informed consent for invasive procedures. 3 Limited English proficiency may also be associated with procedural errors when safety strategies, such as checklists or "time-outs," are not appropriately modified to surmount language barriers, as was apparently the case here. We suggest that operating room checklists prompt the staff to call for professional interpreters when the patient has limited English proficiency; this will allow both the surgeon and the staff to follow routine safety protocols. This modification has been incorporated into informed-consent checklists elsewhere. 4 In addition, although Ring may have excellent Spanish skills, many physicians choose to get by with limited skills, thereby placing patients at risk. 5,6 Hospitals will need to increase their capacity to provide timely professional interpretation if they wish to eliminate ethnic disparities in patient safety.University of California, San Francisco San Francisco, CA afernandez@medsfgh.ucsf.edu No potential conflict of interest relevant to this letter was reported. The discussants reply: Fernandez et al. detail the importance of limited English proficiency as a contributing factor to the case we reported. Our hospital policy for medical-interpretation services discourages the use of staff members as medical interpreters. We identified multiple opportunities to get our medical-interpreter service involved in mitigating the risks identified here. These opportunities included having the surgeon's office schedule an interpreter for the day of surgery, paging the available interpreter at the time of surgery, and using our phone and video interpreter programs. As noted by Fernandez et al., availability is not enough. We need to hardwire the use of interpreter services into our clinical operations. The suggestion to include an additional element of safety related to universalprotocol performance in procedures involving patients with limited English proficiency is an excellent one that we will incorporate into an upcoming policy revision. Case Records of the Massachusetts General Quantifying Oxidant-Scavenging Ability of BloodTo the Editor: Screening the voluminous literature describing the quantifications of antioxidant levels in patients with various clinical disorders and after the administration of supplements has revealed that nearly all the studies have exclusively involved measurements in plasma. Since the classic tests have mainly quantified levels of low-molecular-weight antioxidants and albumin in human plasma but not those associated with erythrocyte enzymes and hemoglobin, such studies have not taken into account the ro...

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Section: Quantifying Oxidant-scavenging Ability Of Bloodmentioning

confidence: 99%

Quantifying Oxidant-Scavenging Ability of Blood

Ginsburg¹,

Kohen²,

Koren³

2011

N Engl J Med

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“…Several studies have demonstrated the role of salivary UCL, [30][31][32] although little research has been published that assesses salivary UCL as an index of psychologic stress. We previously reported that the change in salivary UCL before and after an experimental examination might be a new sensitive biomarker of acute psychologic stress.…”

Section: Discussionmentioning

confidence: 99%

Effect of Psychologic Stress on Peroxidase and Thiocyanate Levels in Human Saliva Detected by Ultraweak Chemiluminescence

Goi

Takagi

Hirai

et al. 2007

JOURNAL OF HEALTH SCIENCE

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Saliva sampling has the advantage of being noninvasive and stress free. Based on a recent study, salivary ultraweak chemiluminescence (UCL) is a new biomarker of psychologic stress. However, it is not clear what causes changes in the UCL level and whether the change is biologically significant. We investigated the candidates for salivary UCL induced by psychological stressors and discuss the physiologic function of these candidates. Volunteers completed a questionnaire and then performed the Kraepelin test. Saliva was sampled just before, immediately after, and 30 min after the stress exposure. The UCL of saliva significantly increased just after stress exposure (1.56-fold) and returned to prestress levels after 30 min. The concentration of secretory immunoglobulin A also increased significantly and the change in both biomarkers was rapid. Similar significant changes were observed in salivary peroxidase activity and the concentration of thiocyanate (SCN − ). On the other hand, the levels of amylase activity did not significantly increase and the concentration of cortisol increased slowly. Moreover, in the reconstitution experiment, UCL was generated at the same level by a mixture of peroxidase and SCN − at physiologic concentrations. In conclusion, we determined that the Kraepelin test as a mental arithmetic task elicited a significant response in the body and this response can be calculated using salivary UCL. Furthermore, SCN − and peroxidase in the saliva play a key role in salivary UCL.

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“…Zum einen ist es in der Lage, andere, niedermolekulare Antioxidantien zu regenerieren und sozusagen zu «recyceln». Damit könnte es einen wichtigen Beitrag zur Aufrechterhaltung des antioxidativen Netzwerks leisten [18,22]. Zum anderen induzierte der Padma 28-Extrakt den durch Nrf-2 vermittelten ARE(antioxidant response element)-Signalweg [13].…”

Section: Oxidativer Stressunclassified

Pathogenese und pleiotrope Behandlungsansätze bei diabetischen Folgeerkrankungen - Übersicht über Wirkmechanismen von Padma® 28

Vennos¹,

Loepfe²

2014

Schweiz Z Ganzheitsmed

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Diabetes mellitus Typ 2 hat aufgrund seiner hohen Prävalenz, seines chronischen Verlaufs und seiner Folgeerkrankungen eine grosse medizinische, aber auch eine sozioökonomische Bedeutung. Es handelt sich um eine komplexe Erkrankung mit einer Vielzahl an pathogenen Faktoren, wobei neben der metabolischen Störung vor allem auch chronisch entzündliche Prozesse beteiligt sind. Oxidativer Stress und die Bildung von Advanced Glycation Endproducts (AGE) spielen eine zentrale Rolle bei der Entstehung und dem Fortschreiten diabetischer Folgeerkrankungen, wie z.B. mikro- und makrovaskulärer Störungen, akzelerierter Arteriosklerose, diabetischer Neuro-, Nephro- und Retinopathie sowie anderer assoziierter chronisch entzündlicher Erkrankungen. Die pathogenen Mechanismen sind komplex; sie beinhalten redundante Signalwege sowie positive Rückkoppelungen und verstärken sich gegenseitig. Daher sind zur Behandlung und Prävention vor allem Arzneimittel geeignet, die ebenfalls ein netzwerkartiges, pleiotropes Wirkmuster haben und an mehreren Stellen im Krankheitskomplex angreifen. Phytotherapeutika besitzen in der Regel eine solche komplexe Wirksignatur. Ein Beispiel für ein solches Phytotherapeutikum mit komplexer Signatur ist das Arzneimittel Padma® 28, das in der Schweiz nach den Traditionen der Tibetischen Medizin hergestellt wird. In der vorliegenden Arbeit werden die Wirkmechanismen dieses Vielstoffgemischs in den Kontext der pathogenen Vorgänge des Diabetes mellitus und seiner Folgeerkrankungen gestellt und diskutiert. Unter diesen scheinen vor allem vielfältige direkte und indirekte antioxidative Effekte, die Hemmung der Bildung von AGE und Advanced Oxidation Protein Products (AOPP) sowie die antientzündliche Aktivität im Vordergrund zu stehen. Das Wirkprofil von Padma 28 scheint damit über arterielle Durchblutungsstörungen hinaus auch die bei Diabetes verstärkten und beschleunigten pathogenen Prozesse günstig beeinflussen zu können. Die Resultate weisen auf einen möglichen Nutzen des Präparats auch bei Diabetes-bedingten vaskulären Störungen und assoziierten chronisch entzündlichen Erkrankungen hin.

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Novel chemiluminescence-inducing cocktails, part II: Measurement of the anti-oxidant capacity of vitamins, thiols, body fluids, alcoholic beverages and edible oils

Cited by 35 publications

References 9 publications

Quantifying Oxidant-Scavenging Ability of Blood

Quantifying Oxidant-Scavenging Ability of Blood

Effect of Psychologic Stress on Peroxidase and Thiocyanate Levels in Human Saliva Detected by Ultraweak Chemiluminescence

Pathogenese und pleiotrope Behandlungsansätze bei diabetischen Folgeerkrankungen - Übersicht über Wirkmechanismen von Padma® 28

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