2016
DOI: 10.18632/aging.101070
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Abstract: Spermatogenesis is a complex process producing haploid spermatozoa, and the formation of lipid droplets (LDs) within Sertoli cells is critical to maintaining normal spermatogenesis. However, the utilization of LDs within Sertoli cells is still largely unknown. In the present study, proliferation of spermatogonial cells had begun in May, whereas the meiotic cells occurred predominately in July and majority of spermiogenic cells were observed in the seminiferous tubules in October. However, TEM and Oil Red O sta… Show more

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Cited by 13 publications
(12 citation statements)
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References 43 publications
(52 reference statements)
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“…These results suggest that lipophagy characteristics can be determined both in fasted fish and cells. It should be noted that, beside mammals, lipophagy has also been reported during spermatogenesis in the Chinese soft-shelled turtle (Ahmed et al, 2016). Therefore, it is reasonable to suggest that lipophagy might be an evolutionarily conserved basic cellular process in vertebrates.…”
Section: Resultsmentioning
confidence: 99%
“…These results suggest that lipophagy characteristics can be determined both in fasted fish and cells. It should be noted that, beside mammals, lipophagy has also been reported during spermatogenesis in the Chinese soft-shelled turtle (Ahmed et al, 2016). Therefore, it is reasonable to suggest that lipophagy might be an evolutionarily conserved basic cellular process in vertebrates.…”
Section: Resultsmentioning
confidence: 99%
“…TEM was performed as previously described [ 40 ]. Briefly, the samples were fixed in 2.5% glutaraldehyde overnight at 4°C, post-fixed for 1.5 h in buffered 1% osmium tetroxide, dehydrated in a graded series of ethyl alcohol or acetone, and embedded in LX 112 epoxy resin.…”
Section: Methodsmentioning
confidence: 99%
“…. The TEM was referred to a previous study [26]. Tissues were soaked in 2.5% glutaraldehyde over 24 h. The fixed intact follicles were dehydrated in gradient ethanol and fixed in 1% osmium tetroxide buffer for 1.5 h, then dehydrated in acetone, and embedded in LX-112 epoxy resin.…”
Section: Transmission Electron Microscopy (Tem)mentioning
confidence: 99%