Novel carbon quantum dots from egg yolk oil and their haemostatic effects

Abstract

In this study, the properties of egg yolk oil (EYO) were investigated. Water extraction, dialysis, and ultrafiltration were used to extract and purify EYO, and microscopy, spectrophotometry, and chromatography were used to identify carbon dots (CDs) present in EYO (EYO CDs). Morphology analyses demonstrated that CDs were almost spherical, with an average size of <10 nm, a lattice spacing of 0.267 nm, and a composition of mainly C, O, and Fe. The solution showed bright blue fluorescence at 365 nm. Tail haemorrhaging and liver haemorrhaging experiments showed that CD-treated mice had significantly shorter bleeding times than did control mice. Coagulation assays suggested that EYO CDs stimulate the intrinsic blood coagulation system and activate the fibrinogen system. Thus, EYO CDs possess the ability to activate haemostasis, which may lead to further investigations of this ingredient of traditional Chinese medicine.

Haemostatic bioactivity of novel Schizonepetae Spica Carbonisata-derived carbon dots via platelet counts elevation

“…Nowadays, researches on self-bioactivity of CDs are on the rise [25][26][27][28][29][30], however, more extensive and deep-going explorations of this area are still needed. In this study, we evaluated the component variation and excluded the interference of small molecular organic compounds using an HPLC method.…”

“…With dramatic properties, such as favourable photoluminescence, excellent chemical stability and hypotoxicity, water dispersity and biocompatibility [9][10][11][12][13][14][15][16][17], CDs has been widely applied in medical domain [18], including drug delivery [19,20], bio-imaging [21,22], optical imaging and sensing [23,24]. Nowadays, researches on self-bioactivity of CDs are on the rise, for example, peroxidase-like activity [25], haemostatic efficacy [26][27][28][29] and selective antibacterial activity against Porphyromonas gingivalis [30], however, more extensive and deep-going explorations of this area are still needed.…”

Chemical structure and in vitro cellular uptake of luminescent carbon quantum dots prepared by solvothermal and microwave assisted techniques

“…High number of precursors have been discussed as possible sources for the preparation of these luminescent materials. These range from graphite [4], and carbon nanotubes [2,5] to simple organic materials, like sugars [6], citric acid [7], ethylene diamine [8], amino acids [9] as well as more complex organic sources like wood [10], milk [11], petroleum coke [12] and eggs [13]. Numerous methods have been described for the preparation of carbon nanodots [14,15].…”

“…Bottom-up synthesis methods are usually based on the thermal decomposition of organic materials by microwave assisted pyrolysis or hydrothermal conversion [20,21,22,19,23]. This latter method has gathered distinguished attention as it enables the use of a wide variety of precursors from organic compounds to natural products and offers an economic way of synthesis suitable for both small and large scale [24,25,13,26,27].…”

Enhancing photoluminescence of carbon quantum dots doped PVA films with randomly dispersed silica microspheres

“…They can be synthesized with facile and low cost methods such as electrochemical fabrication 7 and solution phase synthesis 5 . They can also be made from low cost raw materials, including egg yolk oil 13 , waste papers 14 and tomatoes 15 . CDs have merits of long term stability 16 , low environmental and biological toxicity 9 , broad band optical absorption 17 and good electrochemiluminescence activity 18 .…”