Novel biomarkers in the diagnosis of acute coronary syndromes: the role of circulating miRNAs

Rognoni, Andrea; Cavallino, Chiara; Lupi, Alessandro; Secco, Gioel Gabrio; Veia, Alessia; Bacchini, Sara; Rosso, R.; Rametta, Francesco; Bongo, Angelo Sante

doi:10.1586/14779072.2014.953483

Cited by 11 publications

(8 citation statements)

References 50 publications

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“…In the cardiovascular system, miRNAs have been shown to play crucial roles in pathophysiological conditions, such as endothelial dysfunction, inflammation, apoptosis, and angiogenesis . Several studies highlighted the potential of circulating miRNAs as diagnostic and prognostic biomarkers in ACS . Yet, most of these studies used samples taken at the time of reperfusion to determine miRNAs, rather than samples taken upon initial admission to the Emergency Unit, when diagnostic uncertainty is most evident.…”

Section: Introductionmentioning

confidence: 99%

Serum miRNA-499 and miRNA-210: A potential role in early diagnosis of acute coronary syndrome

et al. 2016

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In clinical practice, there is still a need for novel biomarkers, which can reliably rule in or rule out acute coronary syndrome (ACS) immediately on admission. This is of particular interest in patients with unstable angina (UA) and non-ST-segment elevation myocardial infarction (NSTEMI) in whom diagnostic uncertainty is high. The aim of the present study is to evaluate the potential role of miRNA-499 and miRNA-210 as novel molecular biomarkers for early diagnosis of UA and NSTEMI suspected patients presented at the emergency unit. A total of 110 patients presenting to the intensive care unit (ICU) within 24 h of onset of chest pain suggestive of ACS were enrolled in the study. They included 37 UA, 48 NSTEMI and 25 noncardiac chest pain (NCCP) patients. Immediately at enrollment, blood samples were taken for estimation of serum miRNA-499 and miRNA-210 expression levels by real time PCR. miRNA-499 and miRNA-210 expression levels were significantly increased in UA and NSTEMI patients compared with NCCP patients (P < 0.001). Receiver operating characteristic (ROC) curve analysis revealed that the area under curve (AUC) of miR-499 for the diagnosis of UA and NSTEMI was 0.98 and 0.97, respectively; while the AUC of miRNA-210 was 0.84 and 0.90, respectively. The important finding of our study was that the AUC of miRNA-499 for the diagnosis of ACS patients with symptoms onset <3 h was 0.89, while the AUC of miRNA-210 was 0.86. Interestingly, combining miRNA-499 and miRNA-210 significantly improved the diagnostic value by increasing the AUC to 0.96, P < 0.001. In conclusion, serum miRNA-499 and miRNA-210 are associated with UA and NSTEMI and with those presenting within 3 h of symptom onset. Both miRNAs might be potentially novel biomarkers for accelerating the diagnosis of ACS patients in emergency unit. V C 2016 IUBMB Life, 68(8):673-682, 2016

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Section: Introductionmentioning

confidence: 99%

Serum miRNA-499 and miRNA-210: A potential role in early diagnosis of acute coronary syndrome

et al. 2016

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“…Previous studies have suggested that miRNAs are involved in the pathogenesis of various cardiovascular diseases, including MI, heart failure, atherosclerosis and acute coronary syndromes (20,21). In addition, miRNAs have been shown to be key regulators of cardiac development, angiogenesis and vascular development (22). A previous study reported that miRNAs circulated in the blood of patients with cardiovascular diseases in a stable form (23); therefore, due to this stability, circulating miRNAs may serve as novel biomarkers for the diagnosis of cardiovascular diseases.…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of microRNA involved in unstable angina using gene-chip analysis

Sun

Zhou

et al. 2016

Experimental and Therapeutic Medicine

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Increasing evidence has suggested that microRNA (miRNA) may play a role in the pathogenesis of cardiovascular disease, which has led to a greater understanding of the complex pathophysiological processes underlying unstable angina (UA). The present study aimed to investigate changes in the miRNA expression profiles of patients with UA using gene-chip analysis, in order to further elucidate the pathogenesis of UA. Total RNA was extracted and purified from plasma samples collected from patients with UA and healthy controls. The samples underwent microarray analysis using an Exiqon miRCURY LNA™ microRNA Array. Differentially expressed miRNAs were identified by volcano plot filtering, and were validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In addition, functional annotation of the differentially expressed miRNAs involved gene ontology analyses. Among the 212 miRNAs differentially expressed between the two groups, 82 were upregulated and 130 were downregulated. Notably, the results of the RT-qPCR were consistent with the gene-chip results. The miRNAs identified in the present study may be potential novel biomarkers for the prevention and early diagnosis of UA. Furthermore, the results of the present study suggested that UA occurs as a result of complex and dynamic processes regulated by numerous factors, including multiple miRNAs.

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“…MicroRNAs emerge as promising sensitive and specific biomarkers for cardiovascular disease. 19 , 20 A recent study proved certain circulating miRNAs can distinguish TTC from STEMI patients. 21 In addition, the significant up-regulation of the stress- and depression-related miRNAs suggests a close connection of TTC with neuropsychiatric disorders.…”

Section: Discussionmentioning

confidence: 99%

Takotsubo cardiomyopathy: A potentially serious trap (Data from the International Takotsubo Cardiomyopathy Registry)

Wagdy¹,

ElMaghawry²

2015

Global Cardiology Science and Practice

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Takotsubo cardiomyopathy (TTC) is an acute cardiac condition characterized by transient left ventricular dysfunction with wall motion abnormalities, most commonly in the form of apical ballooning. Despite being considered as a generally benign condition, many studies have emphasized potentially sinister outcomes associated with TTC. In this article, we review the most recent results of the International Takotsubo Registry, which investigated the clinical features, prognostic predictors, and outcomes of 1750 patients.

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Novel biomarkers in the diagnosis of acute coronary syndromes: the role of circulating miRNAs

Cited by 11 publications

References 50 publications

Serum miRNA-499 and miRNA-210: A potential role in early diagnosis of acute coronary syndrome

Serum miRNA-499 and miRNA-210: A potential role in early diagnosis of acute coronary syndrome

Screening and identification of microRNA involved in unstable angina using gene-chip analysis

Takotsubo cardiomyopathy: A potentially serious trap (Data from the International Takotsubo Cardiomyopathy Registry)

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