Airway Cell Biology and Immunopathology 2022
DOI: 10.1183/23120541.lsc-2022.98
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Novel alveolar macrophage-like model (ImmuPHAGE) as a platform for better mechanistic understanding for the fate of inhaled medicines.

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“…In accordance with the literature, alveolar macrophages display phagocytic activity. 36 Therefore, to determine the impact of MC on the phagocytic ability of ImmuPHAGE, cells were incubated for two hours in the dark with uniform 1.0 µm carboxylate-modified microspheres at a ratio of 1 : 30 (cells : particles). Each sample was diluted with 150-200 µL CCM before centrifugation (7 rpm/5 min).…”
Section: Cell Functionality Assessmentmentioning
confidence: 99%
“…In accordance with the literature, alveolar macrophages display phagocytic activity. 36 Therefore, to determine the impact of MC on the phagocytic ability of ImmuPHAGE, cells were incubated for two hours in the dark with uniform 1.0 µm carboxylate-modified microspheres at a ratio of 1 : 30 (cells : particles). Each sample was diluted with 150-200 µL CCM before centrifugation (7 rpm/5 min).…”
Section: Cell Functionality Assessmentmentioning
confidence: 99%