Note on the Determination of Microquantities of Organic Phosphorus

Purification and properties of a haemolysin produced by group A haemolytic streptococci (streptolysin O)

“…Total carbohydrate in protein preparations was determined by the orcin method of Pirie [1936]. Phosphorus was determined by the method of Fiske and Subbarow as modified by Horecker et al [1940]. Arginine was determined according to Weber [1930].…”

Spectrophotometric Ultramicrodetermination of Inorganic Phosphorus and Lipide Phosphorus in Serum.

“…The views of these investigators (2,8,7) are supported by the recovery study on the known control serum. The present method is not recommended when whole blood or serum from badly hemolyzed blood is employed, because the heating and strong acid may hydrolyze some of the organic phosphorus compounds contained in the corpuscles.…”

“…Previous workers (1, 6, 8) expressed the belief that, during heating, some inorganic orthophosphate may be converted into inorganic phosphate in the presence of phosphate esters. However, Buell (8) and Benedict and Theis (2) found no acid-soluble organic phosphorus in the serum filtrate even after its hydrolysis with strong acid or heat.…”

“…The present method, which reduces the amount of serum to 1/m to 1/m Volumetric Determination of Aluminum in Steels, High Temperature Alloys, and Nonferrous Alloys Sir: Investigation of the acidimetric determination of aluminum using potassium fluoride as in Viebóck and Brecher's method (8), and subsequent modifications (1, k, 4, 5) revealed that the method was effective for the determination of aluminum in ores. Adaptation for its use in metal samples seemed practical.…”

“…in its sensitivity is desired. Heat has been applied to intensify the color of the mixture (8), and the method has recently been revived for a sensitive phosphorus assay in lipide column chromatography (1). The procedure described in this paper' involves: (1) increasing the acidity of the sodium trichloroacetate (TCA) filtrate with relatively strong sulfuric acid and adapting the heating modification of Bartlett…”