Normal timing of oligodendrocyte development from genetically engineered,lineage-selectable mouse ES cells

Billon, Noëlle; Jolicoeur, Christine; Ying, Q.Y.; Smith, Austin; Raff, Martin

doi:10.1242/jcs.00049

Cited by 126 publications

(90 citation statements)

References 73 publications

(114 reference statements)

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“…Because studies of nuclear organization rely on analysis at the single cell level, it was important to have a more homogenous population of cells, before and after differentiation. Therefore, we chose to use OS25 ES cells that allow for G418 selection of undifferentiated (Sox2 expressing) cells, and then selection against undifferentiated (Oct4 expressing) cells with Ganciclovir after differentiation (Billon et al 2002). The expression of alkaline phosphatase and SSEA-1 markers, and Oct4, confirmed the undifferentiated state of cells grown in the presence of LIF and G418 (Billon et al 2002;data not shown).…”

Section: Ra Induces a Temporal Program Of Hoxb Gene Expression In Es mentioning

confidence: 92%

“…Undifferentiated OS25 ES cells were maintained on 0.1% gelatin-coated dishes in Glasgows' minimal Eagle medium containing 10% fetal calf serum, nonessential amino acids, 1 mM sodium pyruvate, 0.3 mg/ml L-glutamine, 0.1 mM 2-mercaptoethanol, 1000 U/mL human recombinant LIF, and 100 µg/mL G418 (Billon et al 2002).…”

Section: Es Cells Culture Differentiation and Tsa Treatmentmentioning

confidence: 99%

“…Subsequently, media was supplemented only with Ganciclovir (no RA) for eight more days until day 12. The medium was changed every 2 d during the differentiation protocol (Billon et al 2002).…”

Section: Es Cells Culture Differentiation and Tsa Treatmentmentioning

confidence: 99%

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Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription

Chambeyron

Bickmore²

2004

Genes Dev.

603

567

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The colinearity of genes in Hox clusters suggests a role for chromosome structure in gene regulation. We reveal programmed changes in chromatin structure and nuclear organization upon induction of Hoxb expression by retinoic acid. There is an early increase in the histone modifications that are marks of active chromatin at both the early expressed gene Hoxb1, and also at Hoxb9 that is not expressed until much later. There is also a visible decondensation of the chromatin between Hoxb1 and Hoxb9 at this early stage. However, a further change in higher-order chromatin structure, looping out of genes from the chromosome territory, occurs in synchrony with the execution of the gene expression program. We suggest that higher-order chromatin structure regulates the expression of the HoxB cluster at several levels. Locus-wide changes in chromatin structure (histone modification and chromatin decondensation) may establish a transcriptionally poised state but are not sufficient for the temporal program of gene expression. The choreographed looping out of decondensed chromatin from chromosome territories may then allow for activation of high levels of transcription from the sequence of genes along the cluster.

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Section: Ra Induces a Temporal Program Of Hoxb Gene Expression In Es mentioning

confidence: 92%

Section: Es Cells Culture Differentiation and Tsa Treatmentmentioning

confidence: 99%

See 1 more Smart Citation

Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription

Chambeyron

Bickmore²

2004

Genes Dev.

603

567

View full text Add to dashboard Cite

show abstract

“…To clarify the differences in their capacities to differentiate in vitro, rabbit pluripotent stem cells were induced to undergo further neural differentiation into oligodendrocytes. These are a more mature neural cell type than other neural cells such as neural stem cells, neurons, or astrocytes (32,33). Because the differentiation of pluripotent stem cells into oligodendrocytes was effectively induced by the addition of Noggin, further culture in the presence of Noggin was examined for 20 days (Fig.…”

Section: Rabbit Ips Cells Have a Limited Capacity To Differentiate Intomentioning

confidence: 99%

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Honda

Hatori

Hirose

et al. 2013

Journal of Biological Chemistry

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Background: The quality of induced pluripotent stem (iPS) cells might be inherently worse than embryonic stem cells. Results: Although the differentiation capacity of iPS cells is limited, it can be enhanced. Conclusion: Improving their level of pluripotency alleviated the limited capacity of iPS cells. Significance: This report offers an effective strategy for the development of iPS cell-based research.

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“…Embryonic stem (ES) cells are derived from the inner cell mass of blastocyst-stage embryos, and are pluripotent cells that are able to generate the entire repertoire of cell types in the body. Glial precursors with myelinogenic properties were derived from mouse ES cells [196][197][198]. Human ES cells [199,200] can be directed into neural fate [201,202].…”

Section: Embryonic Stem and Induced Pluripotential Cellsmentioning

confidence: 99%

Cell Therapy for Multiple Sclerosis

Ben‐Hur

2011

Neurotherapeutics

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The spontaneous recovery observed in the early stages of multiple sclerosis (MS) is substituted with a later progressive course and failure of endogenous processes of repair and remyelination. Although this is the basic rationale for cell therapy, it is not clear yet to what degree the MS brain is amenable for repair and whether cell therapy has an advantage in comparison to other strategies to enhance endogenous remyelination. Central to the promise of stem cell therapy is the therapeutic plasticity, by which neural precursors can replace damaged oligodendrocytes and myelin, and also effectively attenuate the autoimmune process in a local, nonsystemic manner to protect brain cells from further injury, as well as facilitate the intrinsic capacity of the brain for recovery. These fundamental immunomodulatory and neurotrophic properties are shared by stem cells of different sources. By using different routes of delivery, cells may target both affected white matter tracts and the perivascular niche where the trafficking of immune cells occur. It is unclear yet whether the therapeutic properties of transplanted cells are maintained with the duration of time. The application of neural stem cell therapy (derived from fetal brain or from human embryonic stem cells) will be realized once their purification, mass generation, and safety are guaranteed. However, previous clinical experience with bone marrow stromal (mesenchymal) stem cells and the relative easy expansion of autologous cells have opened the way to their experimental application in MS. An initial clinical trial has established the probable safety of their intravenous and intrathecal delivery. Short-term follow-up observed immunomodulatory effects and clinical benefit justifying further clinical trials.

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Normal timing of oligodendrocyte development from genetically engineered,lineage-selectable mouse ES cells

Cited by 126 publications

References 73 publications

Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription

Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Cell Therapy for Multiple Sclerosis

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