Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants

Cai, Guangqin; Fan, Chuchuan; Liu, Sheng; Yang, Qingyong; Li, Dongxu; Wu, Jian; Li, Jianwu; Zhou, Yongming; Guo, Liang; Wang, Xuemin

doi:10.1111/nph.16473

Cited by 29 publications

(32 citation statements)

References 75 publications

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“…The C-terminal HA tag was fused upstream of the terminator manually by adding its coding sequence to the reverse primer. The details for gene cloning, plant transformation, putative transgenic plants identi cation were performed as described previously [21,22]. Camelina plants were grown in greenhouse at 21 °C with approximately 16 hr light.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…There were 18 biological replicates with a completely randomized block design for each transgenic line. The greenhouse condition and plants managements were as described previously [21,22]. Measurements of owering time, plant height, branch number, thousand-seed weight, and plant yield per plant were performed as described [23].…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Total protein extraction and immunoblotting were performed as described previously [22]. Protein concentrations were measured using the Bradford assay (Bio-Rad, 500 − 0205).…”

Section: Immunoblotting and Transcript Analysismentioning

confidence: 99%

“…Seed oil content and FA composition were determined as described previously [21,22]. Fatty acid methyl esters (FAMEs) from TAG were identi ed by comparing their retention times with known standards.…”

Section: Seed Oil Content and Fatty Acid Composition Analysesmentioning

confidence: 99%

“…Polar lipids were extracted and analyzed by ESI-MS/MS based on a method described previously [22,26].…”

Section: Lipid Extraction and Pro Lingmentioning

confidence: 99%

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Increased Expression of Fatty Acid and ABC Transporters Enhances Seed Oil Production in Camelina

Cai

Wang

Kim

et al. 2020

Preprint

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Background: Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results: Here we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing siliques, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis was increased in developing seeds of FAX1- and/or ABCA9-OEs. Conclusions: These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Total protein extraction and immunoblotting were performed as described previously [22]. Protein concentrations were measured using the Bradford assay (Bio-Rad, 500 − 0205).…”

Section: Immunoblotting and Transcript Analysismentioning

confidence: 99%

Section: Seed Oil Content and Fatty Acid Composition Analysesmentioning

confidence: 99%

“…Polar lipids were extracted and analyzed by ESI-MS/MS based on a method described previously [22,26].…”

Section: Lipid Extraction and Pro Lingmentioning

confidence: 99%

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Increased Expression of Fatty Acid and ABC Transporters Enhances Seed Oil Production in Camelina

Cai

Wang

Kim

et al. 2020

Preprint

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Non-specific phospholipase C (NPC): an emerging class of phospholipase C in plant growth and development

Nakamura

Ngo

2020

J Plant Res

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Non-specific phospholipase C (NPC) is a novel class of phospholipase C found only in bacteria and higher plants. NPC hydrolyzes major phospholipid classes such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE) to produce diacylglycerol (DAG) and a corresponding phosphate-containing polar head group. Originally known as a toxin in certain bacteria to invade the host cell, this class of phospholipase has been well-investigated in bacteriology. Since the first discovery of eukaryotic NPC in Arabidopsis in 2005, this emerging class of phospholipase has received greater attention in plant biology in elucidating the biochemical characteristics and physiological function in the context of plant growth regulation and stress response. Particularly in the last few years, there has been significant progress made in understanding the fundamental character of 6 NPC isoforms in Arabidopsis, as well as novel function in other plant models. Now that research with plant NPC is entering into a new phase, this review aims to summarize recent progress in plant NPC along with some future perspectives.

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Exploring the plant lipidome: techniques, challenges, and prospects

Liu,

Li,

Chen

et al. 2024

Adv. Biotechnol.

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Plant lipids are a diverse group of biomolecules that play essential roles in plant architecture, physiology, and signaling. To advance our understanding of plant biology and facilitate innovations in plant-based product development, we must have precise methods for the comprehensive analysis of plant lipids. Here, we present a comprehensive overview of current research investigating plant lipids, including their structures, metabolism, and functions. We explore major lipid classes, i.e. fatty acids, glyceroglycolipids, glycerophospholipids, sphingolipids, and phytosterols, and discuss their subcellular distributions. Furthermore, we emphasize the significance of lipidomics research techniques, particularly chromatography-mass spectrometry, for accurate lipid analysis. Special attention is given to lipids as crucial signal receptors and signaling molecules that influence plant growth and responses to environmental challenges. We address research challenges in lipidomics, such as in identifying and quantifying lipids, separating isomers, and avoiding batch effects and ion suppression. Finally, we delve into the practical applications of lipidomics, including its integration with other omics methodologies, lipid visualization, and innovative analytical approaches. This review thus provides valuable insights into the field of plant lipidomics and its potential contributions to plant biology.

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Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants

Cited by 29 publications

References 75 publications

Increased Expression of Fatty Acid and ABC Transporters Enhances Seed Oil Production in Camelina

Increased Expression of Fatty Acid and ABC Transporters Enhances Seed Oil Production in Camelina

Non-specific phospholipase C (NPC): an emerging class of phospholipase C in plant growth and development

Exploring the plant lipidome: techniques, challenges, and prospects

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