Nonlinear microscopy of chitin and chitinous structures: a case study of two cave-dwelling insects

Rabasović, Mihailo D.; Pantelić, Dejan; Jelenković, Branislav; Ćurčić, Srećko; Vrbica, Maja; Lazović, Vladimir; Ćurčić, B.P.M.; Krmpot, Aleksandar J.

doi:10.1117/1.jbo.20.1.016010

Cited by 46 publications

(31 citation statements)

References 26 publications

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“…The homemade two-photon microscopy experimental setup is similar to that reported in our previous study. 31 The schematic of the setup is given in Fig. 1 pulses is generated by the Ti:Sapphire laser (Coherent, Mira 900-F).…”

Section: Experimental Setup For 2pementioning

confidence: 99%

Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy

et al. 2017

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The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label- and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells’ shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.

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Section: Experimental Setup For 2pementioning

confidence: 99%

Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy

et al. 2017

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“…In practice, we operated above threshold to enable reliable and repeatable laser‐drawing. That is why we achieved the minimum cut width which is considerably above the lateral resolution of our femtosecond system . Based on that and the average size of the wing‐scale (~50 × 100 μm 2 ), we estimated the information capacity of a single scale at about 3000 bits, providing that the damaged spot is treated as binary 1, and undamaged as binary 0.…”

Section: Optical Document Protection With Wing Scalesmentioning

confidence: 97%

Naturally safe: Cellular noise for document security

Pavlović

Rabasović

Krmpot

et al. 2019

Journal of Biophotonics

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Modern document protection relies on the simultaneous combination of many optical features with micron and submicron structures, whose complexity is the main obstacle for unauthorized copying. In that sense, documents are best protected by the diffractive optical elements generated lithographically and mass‐produced by embossing. The problem is that the resulting security elements are identical, facilitating mass‐production of both original and counterfeited documents. Here, we prove that each butterfly wing‐scale is structurally and optically unique and can be used as an inimitable optical memory tag and applied for document security. Wing‐scales, exhibiting angular variability of their color, were laser‐cut and bleached to imprint cryptographic information of an authorized issuer. The resulting optical memory tag is extremely durable, as verified by several century‐old insect specimens still retaining their coloration. The described technique is simple, amenable to mass‐production, low cost and easy to integrate within the existing security infrastructure.

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“…Although much useful information can be derived from these simple experiments, not many insect biological tissues have been characterized with multiphoton microscopy. Some other example are the two‐pronged bristletails Plusiocampa christiani and Pheggomisetes ninae as well as the the fire‐chaser beetle Melanophila acuminata .…”

Section: Nonlinear Optical Effects In Biophotonic Structuresmentioning

confidence: 99%

Linear and nonlinear optical effects in biophotonic structures using classical and nonclassical light

Verstraete

Mouchet

Verbiest

et al. 2018

Journal of Biophotonics

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In this perspective article, we review the optical study of different biophotonic geometries and biological structures using classical light in linear and nonlinear regime, especially highlighting the link between these morphologies and modern biomedical research. Additionally, the importance of nonlinear optical study in biological research, beyond traditional cell imaging is also highlighted and described. Finally, we present a short introduction regarding nonclassical light and describe the new future perspective of quantum optical study in biology, revealing the link between quantum realm and biological research.

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Nonlinear microscopy of chitin and chitinous structures: a case study of two cave-dwelling insects

Cited by 46 publications

References 26 publications

Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy

Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy

Naturally safe: Cellular noise for document security

Linear and nonlinear optical effects in biophotonic structures using classical and nonclassical light

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