Noninvasive Biodegradable Nanoparticles-in-Nanofibers Single-Dose Ocular Insert:
 In Vitro
 ,
 Ex Vivo
 and
 In Vivo
 Evaluation

Khalil, Islam A.; Ali, Isra H.; El‐Sherbiny, Ibrahim M.

doi:10.2217/nnm-2018-0297

Cited by 42 publications

(22 citation statements)

References 48 publications

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“…Tensile strength was tested at 5 kN with 10 mm/min rate using universal testing machine (AGX-PLUS, Shimadzu, Japan). Each sample was cut into a rectangle with thickness × width × length (0.5 × 5 × 20 mm) . Porosity of the fabricated NFs (1 cm × 1 cm of known weight) was investigated using a pycnometer (Ultrapyc 1200e, Quantachrome Instruments).…”

Section: Methodsmentioning

confidence: 99%

Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration

Yahia

Khalil

El‐Sherbiny

2019

ACS Appl. Mater. Interfaces

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Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity. The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing. The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM). Apatite deposition was evaluated using simulated body fluid (SBF). The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine. SEM images exhibited a porous three-dimensional structure with NF diameters of 514−4745 nm and 68−786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively. Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8. This was confirmed by measuring the consumption of calcium and phosphorous ions from SBF. Thermal stability of scaffolds was confirmed using DSC and TGA. Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young’s modulus of 17.53 ± 3.24 MPa. The scaffolds’ porous structure enhanced both cell propagation and proliferation. Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment. Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds. Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days. In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.

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Section: Methodsmentioning

confidence: 99%

Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration

Yahia

Khalil

El‐Sherbiny

2019

ACS Appl. Mater. Interfaces

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“…The formulations were characterized in vitro, ex vivo, and in vivo. The results showed improved bioavailability, low risk of toxicity, and prolonged drug release over 10 days [ 56 ]. Two polymer series, polycaprolactone (PCL) and polyvinyl alcohol (PVA), were used to formulate biodegradable polymeric patches containing timolol maleate and dorzolamide hydrochloride for cul-de-sac insertion.…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review of Drug-Loaded Electrospun Nanofiber-Based Ophthalmic Inserts

Omer

Zelkó

2021

Pharmaceutics

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Currently, ocular inserts and nanoparticles have received much attention due to the limited bioavailability of conventional eye preparations and the toxicity problems of systemic drug administration. The current systematic review aims to present recent studies on the use of electrospun nanofiber-based ocular inserts to improve the bioavailability of drugs used for different ophthalmic diseases. A systematic search was performed in PubMed, Ovid Medline, Web of Science, ScienceDirect, Scopus, Reaxys, Google Scholar, and Google Patents/Espacenet taking “drug-loaded”, “nanofibers”, and “ophthalmic inserts” and their equivalent terms as keywords. The search was limited to original and peer-reviewed studies published in 2011–2021 in English language. Only 13 out of 795 articles and 15 out of 197 patents were included. All results revealed the success of nanofiber-based ocular inserts in targeting and improved bioavailability. Ocular inserts based on nanofibers can be used as safe, efficient carriers for the treatment of anterior and posterior eye diseases.

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“…The drug release and permeation profile established that ocular insert could render controlled drug release upto 10 days. The authors concluded that the incorporation of NPs into NFs could achieve many advantages such as increased ocular residence due to better contact with conjunctiva, accurate dosage, prolonged drug release, reduced dosing frequency, reduced systemic side effects, and improved bioavailability [ 64 ].…”

Section: Recent Advancements In Ocular Deliverymentioning

confidence: 99%

Drug Delivery Challenges and Current Progress in Nanocarrier-Based Ocular Therapeutic System

Akhter

Ahmad

Alshahrani

et al. 2022

Gels

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Drug instillation via a topical route is preferred since it is desirable and convenient due to the noninvasive and easy drug access to different segments of the eye for the treatment of ocular ailments. The low dose, rapid onset of action, low or no toxicity to the local tissues, and constrained systemic outreach are more prevalent in this route. The majority of ophthalmic preparations in the market are available as conventional eye drops, which rendered <5% of a drug instilled in the eye. The poor drug availability in ocular tissue may be attributed to the physiological barriers associated with the cornea, conjunctiva, lachrymal drainage, tear turnover, blood–retinal barrier, enzymatic drug degradation, and reflex action, thus impeding deeper drug penetration in the ocular cavity, including the posterior segment. The static barriers in the eye are composed of the sclera, cornea, retina, and blood–retinal barrier, whereas the dynamic barriers, referred to as the conjunctival and choroidal blood flow, tear dilution, and lymphatic clearance, critically impact the bioavailability of drugs. To circumvent such barriers, the rational design of the ocular therapeutic system indeed required enriching the drug holding time and the deeper permeation of the drug, which overall improve the bioavailability of the drug in the ocular tissue. This review provides a brief insight into the structural components of the eye as well as the therapeutic challenges and current developments in the arena of the ocular therapeutic system, based on novel drug delivery systems such as nanomicelles, nanoparticles (NPs), nanosuspensions, liposomes, in situ gel, dendrimers, contact lenses, implants, and microneedles. These nanotechnology platforms generously evolved to overwhelm the troubles associated with the physiological barriers in the ocular route. The controlled-drug-formulation-based strategic approach has considerable potential to enrich drug concentration in a specific area of the eye.

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Noninvasive Biodegradable Nanoparticles-in-Nanofibers Single-Dose Ocular Insert: In Vitro , Ex Vivo and In Vivo Evaluation

Cited by 42 publications

References 48 publications

Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration

Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration

A Systematic Review of Drug-Loaded Electrospun Nanofiber-Based Ophthalmic Inserts

Drug Delivery Challenges and Current Progress in Nanocarrier-Based Ocular Therapeutic System

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