Nonequilibrium Collective Dynamics in Photoexcited Lipid Multilayers by Time Resolved Diffuse X-Ray Scattering

Reusch, Tobias; Mai, Dong-Du; Osterhoff, Markus; Khakhulin, Dmitry; Wulff, Michaël; Salditt, Tim

doi:10.1103/physrevlett.111.268101

Cited by 11 publications

(9 citation statements)

References 25 publications

(31 reference statements)

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“…The links between the cellular behaviour and properties of phospholipid membranes are treated in a review by Tanaka 4 . Investigations of such systems have been carried out using a wide range of methods, such as light-scattering 3 , atomic force microscopy (AFM) 5 , X-ray scattering 6–12 as well as elastic 10, 13 and inelastic 7, 14, 15 neutron scattering. An overview of neutron scattering methods used to investigate phospholipid membranes was presented by Fragneto and Rheinstädter 16 , while Salditt focused on X-ray scattering 10 .…”

Section: Introductionmentioning

confidence: 99%

Nanoscale rheology at solid-complex fluid interfaces

Jaksch

Holderer

Gvaramia

et al. 2017

Sci Rep

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Here we present an approach to measure dynamic membrane properties of phospholipid membranes close to an interface. As an example we show results of the membrane dynamics of a phospholipid membrane multilayer-stack on a solid substrate (silicon). On this sample we were able to measure local interaction and friction parameters using Grazing Incidence Neutron Spin Echo Spectroscopy (GINSES), where an evanescent neutron wave probes the fluctuations close to a rigid interface. With this method it is possible to access length scales in the nano to micrometer region as well as energies in the μeV range. Using a new neutron resonator structure we achieved the required intensity gain for this experiment. During our investigations we found an excitation mode of the phospholipid membrane that has not been reported previously and only became visible using the new methodology. We speculate that the energy transported by that undulation can also serve to distribute energy over a larger area of the membrane, stabilizing it. This new methodology has the capability to probe the viscoelastic effects of biological membranes, becoming a new tool for tribology on the nanoscale and has allowed the observation of the hitherto invisible property of phospholipid membranes using neutrons.

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Section: Introductionmentioning

confidence: 99%

Nanoscale rheology at solid-complex fluid interfaces

Jaksch

Holderer

Gvaramia

et al. 2017

Sci Rep

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“…The third term is a harmonic approximation to the interactions between adjacent bilayers which has been shown to be reasonable [24]. Equation 1is a tilt-dependent extension of a discrete free energy functional previously utilized to describe x-ray scattering from membrane stacks [22,23,[25][26][27], originally from liquid crystal literature [28]. The height fluctuation spectrum was derived following standard methodology [14].…”

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confidence: 99%

Experimental Support for Tilt-Dependent Theory of Biomembrane Mechanics

2014

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Recent simulations have indicated that the traditional model for topographical fluctuations in biomembranes should be enriched to include molecular tilt. Here we report the first experimental data supporting this enrichment. Utilizing a previously posited tilt-dependent model, a height-height correlation function was derived. The x-ray scattering from a liquid crystalline stack of oriented fluid phase lipid bilayers was calculated and compared with experiment. By fitting the measured scattering intensity, both the bending modulus K(c)=8.3±0.6×10⁻²⁰ J and the tilt modulus K(θ)=95±7 mN/m were determined for DOPC lipid bilayers at 30 °C.

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“…Compared to other biophysical techniques, neutron beams may offer distinct advantages when studying biological systems at the atomic level. Neutron scattering does not rely on bulky fluorophore or spin label probes, which can alter the physical properties of model membrane systems [ 10 , 12 ]. Instead, neutrons scatter off the nucleus of the atom, with the greatest scattering power coming from light elements commonly found in biological systems, such as carbon, hydrogen, oxygen and nitrogen.…”

Section: Neutron Scatteringmentioning

confidence: 99%

Neutron Scattering at the Intersection of Heart Health Science and Biophysics

Marquardt

Alsop

Rheinstädter

et al. 2015

JCDD

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There is an urgent quest for improved heart health. Here, we review how neutron radiation can provide insight into the molecular basis of heart health. Lower cholesterol, a daily intake of aspirin and supplemental vitamin E are argued to all improve heart health. However, the mechanisms behind these common regimens, and others, are not entirely understood. It is not clear why a daily intake of aspirin can help some people with heart disease, and the benefits of vitamin E in the treatment of reperfusion injury have been heavily debated. The molecular impact of cholesterol in the body is still a hot topic. Neutron scattering experiments present a unique opportunity for biophysicists attempting to address these problems. We review some recently published studies that are advancing our understanding of how cholesterol, vitamin E and aspirin work at the molecular level, by studying the impact of these molecules on the cell membrane. These insights engage the broader health science community with new ways of thinking about these molecules.

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Nonequilibrium Collective Dynamics in Photoexcited Lipid Multilayers by Time Resolved Diffuse X-Ray Scattering

Cited by 11 publications

References 25 publications

Nanoscale rheology at solid-complex fluid interfaces

Nanoscale rheology at solid-complex fluid interfaces

Experimental Support for Tilt-Dependent Theory of Biomembrane Mechanics

Neutron Scattering at the Intersection of Heart Health Science and Biophysics

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