“…The alterations in the histone proteins have been studied using various biophysical and biochemical techniques like radio-labelling [19], NMR spectroscopy [19], fluorescence spectroscopy [20,21], fourier transform infrared spectroscopy [20,21], circular dichroism [20,22], Xray irradiation [23], electrophoresis [20,21,24], Western blotting [12], immunoassays [25], liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization (MALDI-MS/ MS) [13], and reversed-phase HPLC [26] It was in late 70s and in 80s that some workers suggested Schiff base formation and browning reactions in histone proteins on the pattern of other glycated proteins, thus opening vistas of studies on glyoxidative damage to nuclear proteins [27][28][29][30][31][32][33]. The term 'glycohistone' was coined by Jobst et al in 1991 after they detected histone glycation in hepatic nuclei of patients dying of decompensated diabetes [34].…”