Nondestructive Application of Laser-Induced Fluorescence Spectroscopy for Quantitative Analyses of Phenolic Compounds in Strawberry Fruits (<i>Fragaria x ananassa</i>)

Wulf, Janina; Rühmann, Susanne; Rego, I.; Puhl, Iva; Treutter, D.; Zude, Manuela

doi:10.1021/jf072495i

Cited by 33 publications

(10 citation statements)

References 32 publications

(50 reference statements)

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“…In the HPLC activity profile ( Figure 6) peaks with [M+H] + ions at m/z 291.0 and m/z 442.9 in the ESIMS, and with l max 277 and 280 nm in the UV spectra were detected in the active time window. These peaks corresponded to catechin (Wulf et al, 2008) and epicatechin gallate (Salem et al, 2011), respectively. The occurrence of these compounds in V. nilotica has been reported (Khalid et al, 1989b;Dikti Vildina et al, 2017).…”

Section: Dereplication Of Active Principlesmentioning

confidence: 99%

Mining Sudanese Medicinal Plants for Antiprotozoal Agents

et al. 2020

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Neglected tropical diseases are major health hazards in developing countries. Annually, up to 30 million people are affected by either Chagas disease, African trypansomiasis or leishmaniasis, and more than 200 million by malaria. Most of the currently available drugs have drawbacks in terms of toxicity, limited oral availability, development of resistance, or non-affordability. Tropical plants of the arid zones are a treasure chest for the discovery of bioactive secondary metabolites. This study aims to compile Sudanese medicinal plants, validate their antiprotozoal activities, and identify active molecules. We have performed a survey of medicinal plants of Sudan and selected 62 that are being used in Sudanese traditional medicine. From these, we collected materials such as leaves, stem, bark, or fruit. The plant materials were extracted in 70% ethanol and further fractionated by liquid-liquid partitioning using solvents of increasing polarity. This resulted in a library of 235 fractions. The library was tested in vitro against Plasmodium falciparum (erythrocytic stages), Trypanosoma brucei rhodesiense (bloodstream forms), Trypanosoma cruzi (intracellular amastigotes), and Leishmania donovani (axenic amastigotes). Active fractions were also tested for cytotoxicity. Of the 235 fractions, 125 showed growth inhibitory activity >80% at 10 mg/ml, and >50% at 2 mg/ml against at least one of the protozoan parasites. Plasmodium falciparum was the most sensitive of the parasites, followed by T. b. rhodesiense and L. donovani. Only few hits were identified for T. cruzi, and these were not selective. Contrary to expectation based on phylogeny, but in agreement with previous results, a large number of extracts displayed mutual activity against T. brucei and P. falciparum. HPLC-based activity profiling for selected active extracts was performed to identify the bioactive principles. Active compounds identified by dereplication were guieranone A from Guiera senegalensis J.F.Gmel.; pseudosemiglabrin from Tephrosia apollinea (Delile) DC; ellagic acid and quercetin from Terminalia leiocarpa (DC.) Baill.; and catechin, ethyl gallate, and epicatechin gallate from Vachellia nilotica (L.) P.J.H.Hurter & Mabb. Also the extracts of Croton gratissimus var. gratissimus and Cuscuta hyalina Roth ex Schult. exhibited promising antitrypanosomatid activity. This assessment provides a comprehensive overview of Sudanese medicinal plants and supports the notion that they are a potential source of bioactive molecules against protozoan parasites.

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Section: Dereplication Of Active Principlesmentioning

confidence: 99%

Mining Sudanese Medicinal Plants for Antiprotozoal Agents

et al. 2020

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“…Some of the phenolic compounds are fluorescent, and their contributions to the autofluorescence of food products has been reported, including that of apple juices (Poryvkina, Tsvetkova, & Sobolev, 2014;Seiden, Bro, Poll, & Munck, 1996), wine (Airado-Rodríguez, Durán-Merás, Galeano-Díaz, & Wold, 2011;Airado-Rodríguez, Galeano-Díaz, Durán-Merás, & Wold, 2009), honey (Karoui, Dufour, Bosset, & De Baerdemaeker, 2007;Lenhardt, Bro, Zeković , Dramić anin, & Dramić anin, 2015;Sergiel, Pohl, Biesaga, & Mironczyk, 2014) and strawberry fruits (Wulf et al, 2008). Recently, fluorescence spectroscopy coupled with multivariate regression was used to screen the antioxidant contents and the antioxidant capacities of coffee, peppermint extract and tomato paste (Orzel & Daszykowski, 2014;Orzel, Stanimirova, Czarnik-Matusewicz, & Daszykowski, 2015).…”

Section: Introductionmentioning

confidence: 99%

Explorative study of apple juice fluorescence in relation to antioxidant properties

et al. 2016

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Total fluorescence spectra (excitation-emission matrices, EEM) were recorded for a series of commercial apple juices, including clear and cloudy juices produced from concentrate, cloudy juices that were not from concentrate, and freshly squeezed juices. An exploratory study of the spectra with parallel factor analysis (PARAFAC) revealed three groups of fluorophores with different emission properties, and these properties were characterized by excitation/emission maxima at 270/315nm, (310, 370)/455nm, and 430/(550, 680)nm, respectively. A regression analysis of the total fluorescence spectra arranged into three-way arrays using N-way partial least squares regression methods (NPLS1 and NPLS2) and an analysis of the unfolded spectra by partial least squares methods (PLS1 and PLS2) revealed quantitative relations between the fluorescence and antioxidant properties of juices. The best models for the total phenolic contents and total antioxidant capacities were obtained by applying the NPLS1 method to the EEM. The model parameters were as follows: R(2)CV=0.802, RPD=2.3 for the total phenolic content and R(2)CV=0.808 and RPD=2.3 for the total antioxidant capacity. These results show the potential use of fluorescence spectroscopy for screening apple juices for their antioxidant properties.

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“…Laser-induced fluorescence spectroscopy with excitation at 337 nm was applied to detect phenolic compounds in strawberry fruits (Wulf et al, 2008) and polyphenols in apples (Wulf et al, 2005).…”

Section: Laser-induced Fluorescencementioning

confidence: 99%

Fluorescence Tools for Sensing of Quality-Related Phytochemicals in Fruits and Vegetables

Agati¹,

Bilger²,

Cerović³

2020

Sensor-Based Quality Assessment Systems for Fruits and Vegetables

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The quality of fruits and vegetables can be related to particular classes of compounds possessing peculiar optical properties. This aspect has been exploited by developing specific spectroscopic techniques for the non-destructive detection of such compounds, which are also suitable for the product quality monitoring. Among these, fluorescence spectroscopy was preferentially employed for a long time in the labs of the authors. By using various tools, partially developed by the authors, this technique proved to be suitable not only in basic research, but also for applied purposes. In this chapter, we are going to recall basic concepts of fluorescence and fluorescence measurements, give a brief description of different fluorescence methods employed in the acquisition of parameters describing the quality of fruits and vegetables and define what are the quality-related phytochemicals detectable by fluorescence. A report on portable fluorescence sensors available for in-field measurements is presented in subsection 4.3. Finally, some recent applications of the various fluorescence techniques and sensors on quality assessment of both fruits and vegetables are outlined.

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Nondestructive Application of Laser-Induced Fluorescence Spectroscopy for Quantitative Analyses of Phenolic Compounds in Strawberry Fruits (Fragaria x ananassa)

Cited by 33 publications

References 32 publications

Mining Sudanese Medicinal Plants for Antiprotozoal Agents

Mining Sudanese Medicinal Plants for Antiprotozoal Agents

Explorative study of apple juice fluorescence in relation to antioxidant properties

Fluorescence Tools for Sensing of Quality-Related Phytochemicals in Fruits and Vegetables

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