Non-Zygotic Embryogenesis in Hardwood Species

Corredoira, Elena; Merkle, S. A.; Martínez, M. T.; Toribio, M.; Canhoto, Jorge; Correia, Sandra; Ballester, A.; Viéitez, A. M.

doi:10.1080/07352689.2018.1551122

Cited by 59 publications

(85 citation statements)

References 379 publications

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“…Likewise, average shoot length and number of shoots produced from single starting nodes were also genotype specific. The genotypespecific response with some individuals tolerating and performing better than others in a tissue culture environment has been found in other oak tissue culture studies during the establishment, multiplication, and rooting phases (Herrmann and Buscot, 2008;San-Jos e et al, 1988;Vengadesan and Pijut, 2009;Vieitez et al, 1993Vieitez et al, , 1994Vieitez et al, , 2009 and in oak somatic embryogenesis (Corredoira et al, 2019;Vieitez et al, 2012).…”

Section: Establishmentsupporting

confidence: 53%

Tissue Culture Clonal Propagation of Hybrid White Oaks for the Urban Environment

Sax¹,

Bassuk²,

Bridgen³

2019

horts

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A tissue culture system was developed to clonally propagate a series of hybrid white oaks (Quercus L.) at the plant breeding program of the Urban Horticulture Institute (UHI), Cornell University. From 2014 through 2018, 34 genotypes and 1966 individual explants of UHI hybrid white oaks were trialed to determine their capacity to establish, multiply, and root in a tissue culture environment. UHI hybrid oak genotypes were selected based on their known tolerance to stresses common in the urban environment (drought, alkaline soils, pests, and diseases) and their ornamental qualities. Individual genotype was the single largest factor affecting successful establishment, multiplication, stabilization, and rooting of hybrid oaks in vitro. Thirteen clones of hybrid oaks were identified as having the capacity to stabilize and grow continuously in the multiplication phase. Multiplication efficiency rates were dependent on individual genotype. Stabilized genotypes showed the capacity to be re-established during successive years. The tissue culture process was simplified and refined to make the protocols less labor intensive for laboratory technicians using these methods. This study presents a preliminary and promising method for the clonal propagation of oak species and provides a path for cultivar development for plants belonging to the genus Quercus.

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Section: Establishmentsupporting

confidence: 53%

Tissue Culture Clonal Propagation of Hybrid White Oaks for the Urban Environment

Sax¹,

Bassuk²,

Bridgen³

2019

horts

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“…Nowadays, the main limitation for using somatic embryogenesis in trees is that, in many Hardwood species and in almost all Gymnosperm species, the induction of somatic embryos is achieved by using zygotic embryos as an initial explant, which normally have a genetic unknown value (Corredoira et al., 2018a). It would be recommendable to induce somatic embryogenesis in explants from adult specimens when the characteristics of the genotype that is being propagated are known; but then, the capacity to induce somatic embryogenesis decreases considerably (Bonga et al., 2010).…”

Section: Advances On the Application Of Somatic Embryogenesis In Adulmentioning

confidence: 99%

“…A possible way to overcome this problem is to employ adult tissues that have regained juvenile characteristics like floral tissues (e.g., immature inflorescences, petals, floral staminodes, pistils, stamens, or anther teguments) or maternal tissues (e.g., nucellus or inner teguments) (Corredoira et al., 2018a). The induction of somatic embryos is generally easier in these types of explants, as the site of meiosis is closed around the time of meiosis (Bonga et al., 2010).…”

Section: Advances On the Application Of Somatic Embryogenesis In Adulmentioning

confidence: 99%

“…Initially, the objectives were focused on the search within the tree material that had undergone a certain level of rejuvenation. First, a successful protocol for inducing somatic embryos in recalcitrant cases was defined using leaves obtained from shoots derived from forced flushing of branch segments (Ballester et al., 2016). Forced shoots are the result of growth of preformed dormant buds, which are in a state of quiescence in the tree (Vieitez et al., 2012).…”

Section: Advances On the Application Of Somatic Embryogenesis In Adulmentioning

confidence: 99%

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Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

et al. 2019

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Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advantages such as mass propagation, genetic transformation, application of synthetic seed, and cryopreservation of elite genotypes. In this report, the state of the art of somatic embryogenesis in holm oak is reviewed. Factors affecting the induction (plant donor age, type of explant, or plant growth regulators) and maintenance and proliferation of the embryogenic cultures are summarized. Advances on the conversion of somatic embryos into plants and on the acclimatization of these plantlets, as well as the results obtained on the application of the genetic transformation and the cryopreservation procedures to holm oak embryogenic cultures, are also presented.

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“…Following this phase, the progeny may be released as a clonal cultivar. Cloning selected materials allows to capture all selection and improvement gains without involving genetic segregation [7].…”

Section: Introductionmentioning

confidence: 99%

Observations on Somatic Embryogenesis inCoffea arabicaL.

Almeida¹

2020

Coffee - Production and Research

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Somatic embryogenesis contributes to coffee breeding programs. This is a process of asexual reproduction which is based on the concept of cellular totipotency. Each haploid or somatic cell of the plant tissue has the genetic information necessary to generate a complete and functional plant. The somatic embryogenesis can occur either indirectly or directly. Coffea arabica genotypes may respond to direct, indirect, or both. In this species, the indirect somatic embryogenesis is composed of two phases, the callogenesis and the embryogenesis, while the direct pathway occurs in a single phase, without the callogenesis. In Coffea, in general, the indirect pathway is induced by the auxin and cytokinin, and the direct pathway with cytokinin only. C. arabica genotypes usually respond easily to the indirect route with high production of somatic embryos. But these are inefficient by the direct route because they present low production of embryos and the process occurs for a long time. In this review, emphasis will be given to different events that are part of the somatic embryogenesis of C. arabica occurring indirectly and in the direct pathway as well as factors that may affect its control.

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Non-Zygotic Embryogenesis in Hardwood Species

Cited by 59 publications

References 379 publications

Tissue Culture Clonal Propagation of Hybrid White Oaks for the Urban Environment

Tissue Culture Clonal Propagation of Hybrid White Oaks for the Urban Environment

Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

Observations on Somatic Embryogenesis inCoffea arabicaL.

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