Non-viral Gene Disruption by CRISPR/Cas9 Delivery Using Cell-permeable and Protein-stabilizing 30Kc19 Protein

Kim, Yu Jin; Lee, Hyelim; Cha, Hyeonjin; Park, Jae Young

doi:10.1007/s12257-020-0068-8

Cited by 4 publications

(1 citation statement)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…n the field of biotechnology, protein delivery systems have various applications like laboratory applications (gene editing) and clinical applications (drug delivery) [1][2][3][4][5][6][7] . To date, numerous methods have been introduced for efficient intracellular protein delivery in mammalian cells, in which various biomaterials are used as delivery vehicles, including cationic lipids 2 , peptides 3,8,9 , nanoparticles [10][11][12][13] , and decorated liposomes 14,15 .…”

mentioning

confidence: 99%

Identification of efficient prokaryotic cell-penetrating peptides with applications in bacterial biotechnology

et al. 2021

View full text Add to dashboard Cite

In bacterial biotechnology, instead of producing functional proteins from plasmids, it is often necessary to deliver functional proteins directly into live cells for genetic manipulation or physiological modification. We constructed a library of cell-penetrating peptides (CPPs) capable of delivering protein cargo into bacteria and developed an efficient delivery method for CPP-conjugated proteins. We screened the library for highly efficient CPPs with no significant cytotoxicity in Escherichia coli and developed a model for predicting the penetration efficiency of a query peptide, enabling the design of new and efficient CPPs. As a proof-of-concept, we used the CPPs for plasmid curing in E. coli and marker gene excision in Methylomonas sp. DH-1. In summary, we demonstrated the utility of CPPs in bacterial engineering. The use of CPPs would facilitate bacterial biotechnology such as genetic engineering, synthetic biology, metabolic engineering, and physiology studies.

show abstract