Non-synonymous mutations mapped to chromosome X associated with andrological and growth traits in beef cattle

Abstract

BackgroundPrevious genome-wide association analyses identified QTL regions in the X chromosome for percentage of normal sperm and scrotal circumference in Brahman and Tropical Composite cattle. These traits are important to be studied because they are indicators of male fertility and are correlated with female sexual precocity and reproductive longevity. The aim was to investigate candidate genes in these regions and to identify putative causative mutations that influence these traits. In addition, we tested the identified mutations for female fertility and growth traits.ResultsUsing a combination of bioinformatics and molecular assay technology, twelve non-synonymous SNPs in eleven genes were genotyped in a cattle population. Three and nine SNPs explained more than 1% of the additive genetic variance for percentage of normal sperm and scrotal circumference, respectively. The SNPs that had a major influence in percentage of normal sperm were mapped to LOC100138021 and TAF7L genes; and in TEX11 and AR genes for scrotal circumference. One SNP in TEX11 was explained ~13% of the additive genetic variance for scrotal circumference at 12 months. The tested SNP were also associated with weight measurements, but not with female fertility traits.ConclusionsThe strong association of SNPs located in X chromosome genes with male fertility traits validates the QTL. The implicated genes became good candidates to be used for genetic evaluation, without detrimentally influencing female fertility traits.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1595-0) contains supplementary material, which is available to authorized users.

Genetic parameters, genetic trends, and principal component analysis for productive and reproductive traits of Guzera beef cattle

“…Irano et al (2016) performed GWAS for indicators of sexual precocity in Nelore cattle and found that windows with great effects for scrotal perimeter and sexual precocity traits were not superimposed, although there is a moderate genetic correlation between these traits in Nelore cattle (Santana et al, 2015). Camargo et al (2015), studying causal mutations, showed that highly significant SNP for andrological traits were not significant for female reproductive traits. Irano et al (2016) elaborated three hypotheses to try to explain the association between these traits.…”

Short Communication: Population structure of mangrove crab Scylla oceanica in mangrove ecosystem of Tanjung Lesung, Banten, Indonesia

“…IGFBP-3 gene has been investigated on small ruminants such as goats and sheep much more than in cattle. Eight SNPs have been detected in exon 2 of IGFBP-3 gene, 6 of them produce nonsynonymous protein coding or non-synonymous mutation (Sharma et al 2014) which can lead to a significant metabolic abnormality that is desirable or not desirable (Veneroni-Gouveia et al 2015;de Camargo et al 2015). IGFBP-3 gene expression was higher in the liver and longisimus dorsi muscles, thus contributing to the development of these organs (Zhang et al 2015), increased ADG from birth to weaning and weaning weight (Rasouli et al 2016), but there is not enough evidence to suggest that SNPs are associated with milk production (Zhou et al 2016).…”

Candidate mutations used to aid the prediction of genetic merit for female reproductive traits in tropical beef cattle

“…These SNP were selected from the 140 SNP previously described by Fortes et al (2014a), because examination of sequenced genomes of representative sires showed that they were present in our validation populations. Sequenced genomes from Beef CRC sires were described before (Camargo et al, 2015) and were submitted to the 1000-bull genome project (Hayes et al, 2014).…”