Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures

Haller, Dirk; Bode, Christiane; Hammes, Walter P.; Pfeifer, Andréa; Schiffrin, Eduardo J.; Blum, Stéphanie

doi:10.1136/gut.47.1.79

Cited by 424 publications

(318 citation statements)

References 56 publications

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“…As found in former experiments (16,17), apical stimulation of Caco-2 cells by nonpathogenic bacteria leads to an enhanced production of cytokines in the basolateral compartment of the coculture model. In parallel with conditions in the intestine, bacteria and leukocytes are separated from leukocytes by a layer of IEC.…”

Section: Discussionsupporting

confidence: 72%

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Conjugated primary bile salts reduce permeability of endotoxin through intestinal epithelial cells and synergize with phosphatidylcholine in suppression of inflammatory cytokine production

Parlesak

Schaeckeler

Moser

et al. 2007

Critical Care Medicine

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Section: Discussionsupporting

confidence: 72%

“…For this purpose, we used a coculture model that was previously established in our department (16,17). In this model, a layer of differentiated IEC (Caco-2 cells) separated an apical compartment (luminal side) from a basolateral one (blood side).…”

Section: Conjugated Primary Bile Salts Reduce Permeability Of Endotoxmentioning

confidence: 99%

Conjugated primary bile salts reduce permeability of endotoxin through intestinal epithelial cells and synergize with phosphatidylcholine in suppression of inflammatory cytokine production

Parlesak

Schaeckeler

Moser

et al. 2007

Critical Care Medicine

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“…Several lines of evidence in vivo and in vitro indicate that probiotics engage and interact with host immune and other mucosal cells (12,32,33). In this study, consumption of L. salivarius UCC118 Rif was associated with a speci c secretary IgA anti-UCC118 Rif response as detected in saliva by a dilution-controlled, protein-standardised ow cytometric detection system.…”

Section: Discussionmentioning

confidence: 59%

A Randomised Controlled Trial of a Probiotic Lactobacillus Strain in Healthy Adults: Assessment of its Delivery, Transit and Influence on Microbial Flora and Enteric Immunity

Collins

Dunne²,

Murphy³

et al. 2002

Microbial Ecology in Health and Disease

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In severa l intestinal disease states, altered micro ora, impaired gut barrier and:or intestinal in ammation offer a rationale for the effective therapeut ic use of probiotic microorganisms. However , for most candidat e probiotic organisms there is a lack of evidence detailing their characterisation and effects on host ora and immunity. We have previously reported the isolation and characterisation, from surgically resected segment s of the huma n gastrointestinal tract (GIT), of potential probiotic lactic acid bacteria (LAB). We have also described subsequen t animal experimen ts that evaluated the establishment , persistence and localisation of speci c probiotic Lactobacillus strains within the murine intestinal tract, in addition to their ability to in uence the developmen t of murine in ammatory disorders. In these studies, transit and surviva l of Lactobacillus salivarius U CC118 at the ileum was demonstrat ed using enteral tube sampling of six healthy volunteer s following consumption of a single dose (150 ml) of fermented milk-born e probiotic (10 8 colony forming units per ml (CF U :ml)). Subsequently, we performed a randomised controlled trial of 80 volunteer s fed strain UCC118 (10 8 CF U:day for 21 d), using two oral delivery vehicles (fresh milk, n ¾ 20 vs. fermented milk, n ¾ 20; controls, n ¾ 20 for each). Throughout this feeding period , and for up to 100 days following cessation of feeding, the number s of total culturable lactobacilli and of the administered Lactobacillus U CC118 present in faeces were monitored. F ive subjects (5:40; fresh milk, four; fermented milk, one) were still excretin g the probiotic lactobacilli 21 days post-cessatio n of feeding, while one subject (fermented milk) was still colonised up to 100 days after feeding. Consumpt ion of fermented milk-born e UCC118 cells resulted in signi cantly increased levels of faecal-borne enterococci and lactobacilli. N umber s of bi dobacteria, coliforms and bacteroides were not signi cantly altered. In addition, changes in salivary IgA levels against U CC118 cells and increased granulocyt e phagocytic activity were observed following consumption of the fermented milk-borne probiotic. In summary, Lactobacillus UCC118 was found to effectively transit (and persist within) the human intestinal tract, to modify the faeca l ora and to engage the immune system.

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“…Indeed, they found that Caco-2 cells alone are hyporesponsive to challenge with non-pathogenic E. coli and L. sakei. On the contrary, when cultured with leukocytes, Caco-2 cells produce TNF-α and IL-1β after treatment with L. sakei, but also the antiinflammatory transforming growth factor (TGF)-β after L. johnsonii treatment [26].…”

Section: Immunomodulationmentioning

confidence: 99%

Alternatives to in-feed antibiotics in pigs: Evaluation of probiotics, zinc or organic acids as protective agents for the intestinal mucosa. A comparison of in vitro and in vivo results

et al. 2005

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-Weaned pigs are susceptible to frequent infectious diseases. Antibiotics have been used over decades to reduce pathogen infections, but many microorganisms are becoming resistant to antibiotics. Thus, there is an urgent need to find alternatives to in-feed antibiotics. Up to now, various compounds have been used as alternatives, giving promising but sometimes contrasting results. In recent years, the in vitro models of cell culture have been proposed for the screening of various compounds and understanding their mechanisms of action. The intestine is a complex system with a continuous cross-talk among epithelial cells, the local immune system and microflora. Alternatives to in-feed antibiotics may interact with any of these constituents. This review presents data on the protective effects of probiotics, zinc salts and organic acids, used as alternatives to in-feed antibiotics, against pathogen induced damage and inflammatory processes in the intestinal cells and the underlying mechanisms, with an attempt to compare in vitro and in vivo results. All the alternatives tested resulted in effective protection against pathogens by a series of interactions with intestinal cells and pathogens, including the inhibition of adhesion and invasion of pathogens, interference in signalling pathways induced by pathogenic bacteria, maintenance of the epithelial cell cytoskeleton and junctional structure and modulation of the host immune response. microorganismes sont devenus résistants à ces substances. Aussi, est-il urgent de trouver des alternatives aux antibiotiques additifs alimentaires. Jusqu'à présent, diverses alternatives ont été envisagées, certaines donnant des résultats prometteurs, d'autres des résultats variables. Au cours des dernières années, les modèles in vitro de cultures cellulaires ont été proposés pour cribler ces substances et élucider leurs mécanismes d'action. L'intestin est un système complexe faisant intervenir des communications permanentes entre les cellules épithéliales, le système immunitaire local et la microflore. Les alternatives aux antibiotiques peuvent interagir avec n'importe lequel de ces composants. Le présent article fait la synthèse des effets protecteurs et des mécanismes d'action des probiotiques, des sels de zinc et des acides organiques, utilisés comme alternatives aux antibiotiques dans les processus inflammatoires et les altérations cellulaires épithéliales in vitro, induites par des agents pathogènes. Elle tente de comparer ces résultats avec les observations in vivo. Toutes les alternatives évaluées ont été efficaces contre les agents pathogènes, via des mécanismes d'interaction cellules épithéliales-agents pathogènes. Ces mécanismes incluaient l'inhibition de l'adhésion et de l'invasion cellulaire par ces pathogènes, la modulation des mécanismes de signalisation intracellulaire induits par les bactéries pathogènes, le maintien de la structure du cytosquelette et des jonctions serrées des cellules épithéliales, et enfin la modulation des réponses immunitaires de la cellu...

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Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures

Cited by 424 publications

References 56 publications

Conjugated primary bile salts reduce permeability of endotoxin through intestinal epithelial cells and synergize with phosphatidylcholine in suppression of inflammatory cytokine production

Conjugated primary bile salts reduce permeability of endotoxin through intestinal epithelial cells and synergize with phosphatidylcholine in suppression of inflammatory cytokine production

A Randomised Controlled Trial of a Probiotic Lactobacillus Strain in Healthy Adults: Assessment of its Delivery, Transit and Influence on Microbial Flora and Enteric Immunity

Alternatives to in-feed antibiotics in pigs: Evaluation of probiotics, zinc or organic acids as protective agents for the intestinal mucosa. A comparison of in vitro and in vivo results

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