Non-doped and non-modified carbon dots with high quantum yield for the chemosensing of uric acid and living cell imaging

Pang, Xinpei; Yan, Rui; Li, Li; Wang, Panyong; Zhang, Yan; Liu, Yulu; Liu, Pai; Dong, Wen‐Fei; Mei, Qian

doi:10.1016/j.aca.2022.339571

Cited by 16 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following this, urine samples were prepared using synthetic human urine without UA (Figure 5d). Uric acid concentrations in the urine of healthy individuals typically range from 160 to 320 µM [47]. Therefore, we prepared a sample reflecting normal conditions by dissolving 300 µM of uric acid, within the normal range, in artificial urine.…”

Section: Ua Detection In Real Sample: Human Serum and Urinementioning

confidence: 99%

Asterias forbesi-Inspired SERS Substrates for Wide-Range Detection of Uric Acid

Park,

Chai,

Kim

et al. 2023

Biosensors

View full text Add to dashboard Cite

Uric acid (UA), the final metabolite of purine, is primarily excreted through urine to maintain an appropriate concentration in the bloodstream. However, any malfunction in this process can lead to complications due to either deficiency or excess amount of UA. Hence, the development of a sensor platform with a wide-range detection is crucial. To realize this, we fabricated a surface-enhanced Raman spectroscopy (SERS) substrate inspired by a type of starfish with numerous protrusions, Asterias forbesi. The Asterias forbesi-inspired SERS (AF-SERS) substrate utilized an Au@Ag nanostructure and gold nanoparticles to mimic the leg and protrusion morphology of the starfish. This substrate exhibited excellent Raman performance due to numerous hotspots, demonstrating outstanding stability, reproducibility, and repeatability. In laboratory settings, we successfully detected UA down to a concentration of 1.16 nM (limit of detection) and demonstrated selectivity against various metabolites. In the experiments designed for real-world application, the AF-SERS substrate detected a broad range of UA concentrations, covering deficiencies and excesses, in both serum and urine samples. These results underscore the potential of the developed AF-SERS substrate as a practical detection platform for UA in real-world applications.

show abstract

Section: Ua Detection In Real Sample: Human Serum and Urinementioning

confidence: 99%

Asterias forbesi-Inspired SERS Substrates for Wide-Range Detection of Uric Acid

Park,

Chai,

Kim

et al. 2023

Biosensors

View full text Add to dashboard Cite

show abstract

“…13 The linear detection ranges and LOD obtained in the established sensor to detect UA were compared with those in the previously reported studies so as to reveal its performance (Table S2, ESI †). At present, most of the fluorescence detection methods of UA are still based on the indirect detection way in the presence of enzymes, [39][40][41] in contrast, this work realizes the enzyme-free detection. Of note, the data show that the linear detection ranges and LOD are better than or comparable to those of the other methods (Table S2, ESI †).…”

Section: Sensitive and Selective Detection Of Uric Acidmentioning

confidence: 99%

Fabrication of nitrogen-enriched carbon dots with green fluorescence for enzyme-free detection of uric acid

Chai

Mao

et al. 2022

New J. Chem.

View full text Add to dashboard Cite

show abstract

“…Therefore, an accurate, rapid, and sensitive method for the detection of uric acid is important for effective gout control, diagnosis, and therapy. Thus far, various methods such as electrochemical detection [7–9], enzymatic method [10, 11], chromatography [12–14], and fluorescence spectroscopy have been developed for uric acid detection [15, 16]. However, disadvantages such as complicated sample pretreatments, expensive instruments, and insufficient sensitivity and selectivity greatly limit their wide application in complex biological samples [17].…”

Section: Introductionmentioning

confidence: 99%

Photosensitized covalent organic framework as a light‐induced oxidase mimic for colorimetric detection of uric acid

Wang,

Li,

et al. 2024

Luminescence

View full text Add to dashboard Cite

As large numbers of people are suffering from gout, an accurate, rapid, and sensitive method for the detection of gout biomarker, uric acid, is important for its effective control, diagnosis, and therapy. Although colorimetric detection methods based on uricase have been considered, they still have limitations as they produce toxic H2O2 and are expensive and not stable. Here, a novel uricase‐free colorimetric method was developed for the sensitive and selective detection of uric acid based on the light‐induced oxidase‐mimicking activity of a new photosensitized covalent organic framework (COF) (2,4,6‐trimethylpyridine‐3,5‐dicarbonitrile–4‐[2‐(4‐formylphenyl)ethynyl]benzaldehyde COF [DCTP–EDA COF]). DCTP–EDA COF has a strong ability to harvest visible light, and it could catalyze the oxidation of 1,4‐dioxane, 3,3′,5,5′‐tetramethylbenzidine under visible light irradiation to produce obvious color changes. With the addition of uric acid, however, the significant inhibition of the oxidase‐mimicking activity of DCTP–EDA COF remarkably faded the color, and thus uric acid could be colorimetrically detected in the range of 2.0–150 μM with a limit of detection of 0.62 μM (3σ/K). Moreover, the present colorimetric method exhibited high selectivity; uric acid level in serum samples was successfully determined, and the recoveries ranged from 96.5% to 105.64%, suggesting the high accuracy of the present colorimetric method, which demonstrates great promise in clinical analysis.

show abstract

Non-doped and non-modified carbon dots with high quantum yield for the chemosensing of uric acid and living cell imaging

Cited by 16 publications

References 48 publications

Asterias forbesi-Inspired SERS Substrates for Wide-Range Detection of Uric Acid

Asterias forbesi-Inspired SERS Substrates for Wide-Range Detection of Uric Acid

Fabrication of nitrogen-enriched carbon dots with green fluorescence for enzyme-free detection of uric acid

Photosensitized covalent organic framework as a light‐induced oxidase mimic for colorimetric detection of uric acid

Contact Info

Product

Resources

About