Non-destructive vacuum-assisted measurement of lung elastic modulus

Chen, Jiawen; Mir, Seyed Mohammad; Pinezich, Meghan R.; O’Neill, John D.; Guenthart, Brandon A.; Bacchetta, Matthew; Vunjak‐Novakovic, Gordana; Huang, Sarah X.L.; Kim, Jin-Ho

doi:10.1016/j.actbio.2021.06.037

Cited by 7 publications

(4 citation statements)

References 81 publications

(79 reference statements)

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“…Although we did not find a significant correlation between the number of smoking pack years and the YM of tumours, research has shown that smoking causes a significant increase in the stiffness of cadaveric lung tissue in smokers versus nonsmokers [19]. Furthermore, researchers have found differences in the YM of healthy and damaged lung tissue in murine models at the cellular level [20]. Increased cellular turn over and dysplasia could result in the stiffening of normal lung tissue and lung tumours.…”

Section: Commentcontrasting

confidence: 79%

Young’s Moduli of Human Lung Parenchyma and Tumours

2024

J Oncol Res Ther

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Tumour localization of small deep subpleural lesions during VATS depends on the differential tissue stiffness between a tumour and the adjacent tissue. To obtain a quantitative measure of stiffness, we catalogued the Young's Modulus of in situ abnormal lung lesions and adjacent tissue from resected specimens.A 5 to 10 mm section of resected tumour and adjacent lung tissue was placed in a custom indenting elastometer device to measure the Young's Modulus. The device applied a uniform force on the tissue samples to varying levels of tissue displacement -up to 15% of their thickness. The Young's Modulus was calculated from 200 measurements of forces and displacements. Each set of measurements was repeated three times. The Young's Modulus for each tissue histology was assessed by parametric or nonparametric analyses as appropriate.The median [range] Young's Modulus for all lung tumours ; p < 0.001) was significantly higher than adjacent lung tissue (6.12 kPa [1. 65-13.19]; p < 0.001). Adenocarcinomas, squamous cell carcinomas, various metastases, and granuloma/fibromas had Young's Modulus values greater than adjacent lung histologies. This is the first study to report the elastic properties of human lung parenchyma in various disease states and abnormal lesions. There was a significant difference between the Young's Moduli of human lung tumours and parenchyma. These findings may aid in the development of improved intraoperative localization technologies for minimally invasive pulmonary surgeries.

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Section: Commentcontrasting

confidence: 79%

Young’s Moduli of Human Lung Parenchyma and Tumours

2024

J Oncol Res Ther

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“…Similar to lung and other soft tissues, sealant compression moduli increased nonlinearly with increasing strain. [ 27 ] Further, increasing transglutaminase concentration resulted in greater sealant compression moduli across all strain ranges (Figure 3B). To determine Young's modulus and elongation at failure under stretch conditions similar to those the sealant encounters during the respiratory cycle, we performed tension testing (Figure 3C; Figure S3, Supporting Information).…”

Section: Resultsmentioning

confidence: 97%

Lung‐Mimetic Hydrofoam Sealant to Treat Pulmonary Air Leak

Pinezich,

Mir,

Graney

et al. 2024

Adv Healthcare Materials

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Pulmonary air leak is the most common complication of lung surgery, contributing to post‐operative morbidity in up to 60% of patients, yet there is no reliable treatment. Available surgical sealants do not match the demanding deformation mechanics of lung tissue and therefore fail to seal air leak. To address this therapeutic gap, we developed and systematically evaluated a sealant with structural and mechanical similarity to subpleural lung. Our “lung‐mimetic” sealant is a hydrofoam material that has alveolar‐like porous ultrastructure, lung‐like viscoelastic properties (adhesive, compressive, tensile), and lung extracellular matrix‐derived signals (matrikines) to support tissue repair. In biocompatibility testing, the lung‐mimetic sealant showed minimal cytotoxicity and immunogenicity in vitro. Human primary monocytes exposed to sealant matrikines in vitro upregulated key genes (MARCO, PDGFB, VEGF) known to correlate with pleural wound healing and tissue repair in vivo. In rat and swine models of pulmonary air leak, our lung‐mimetic sealant rapidly sealed air leak and restored baseline lung mechanics. Altogether, these data indicate that our lung‐mimetic sealant can effectively seal pulmonary air leak and promote a favorable cellular response in vitro.This article is protected by copyright. All rights reserved

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“…, PhysioSuite, Kent Scientific; tidal volume: 15 mL kg −1 of animal weight; breathing rate: 60–70 breaths per minute). 73,74 In addition, future studies should evaluate the creation of the ALI within the cultured trachea in our bioreactor to guide the differentiation of implanted basal cells into different epithelial cell types. The creation of ALI can be achieved by selectively controlling the infusion of culture medium and airflow through dedicated inlet ports.…”

Section: Discussionmentioning

confidence: 99%