Non-Coding RNAs as Regulators of Myogenesis and Postexercise Muscle Regeneration

Archacka, Karolina; Ciemerych, Maria A.; Florkowska, Anita; Romanczuk, Karolina

doi:10.3390/ijms222111568

Cited by 9 publications

(11 citation statements)

References 145 publications

(193 reference statements)

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“…The development of myogenesis is regulated by epigenetic factors, including DNA methylation, RNA methylation, histone modification, and noncoding RNA [ 35 , 36 , 37 ]. There have been many reports on the research of lncRNA in poultry [ 38 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

Chen

Zhang

Geng-Hua

et al. 2022

Animals

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Chinese Shitou goose is a type of large goose with high meat yield. Understanding the genetic regulation of muscle development in Shitou goose would be beneficial to improve the meat production traits of geese. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the RNA-seq method was used to construct the mRNA and lncRNA expression profiles of Shitou goose myoblasts and myotubes. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified. The alternative mRNA splicing in proliferation and differentiation stages was also analyzed. Notably, pathways enriched in DE-mRNAs, DE-splicing transcripts, and DE-lncRNAs all point to the Wnt signaling pathway, indicating that the Wnt signaling is a key regulatory pathway of muscle development in Shitou goose. We also constructed the interactive network of DE-lncRNAs and DE-mRNAs and revealed some key genes of lncRNAs regulating the proliferation and differentiation of myoblasts. These results provide new insights for the study of the muscle development of the Shitou goose.

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Section: Resultsmentioning

confidence: 99%

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

Chen

Zhang

Geng-Hua

et al. 2022

Animals

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“…During myogenesis, the activation of muscle-specific transcription factors and the reprogramming of skeletal myogenesis genes are necessary for precursor cells to differentiate into myogenic lineages and form new myofibers [ 48 , 49 ]. In recent years, miRNAs have been determined to play a fundamental role in directing and modulating satellite cell/myoblast differentiation [ 5 , 49 , 66 , 92 , 93 , 94 ]. Mice with reduced muscle miRNAs (eliminated Dicer activity, specifically in muscles) showed decreased skeletal muscle mass and increased myoblast apoptosis, as well as abnormal myofiber morphology [ 95 ].…”

Section: Exosomes In Skeletal Muscle Repair and Regenerationmentioning

confidence: 99%

The Therapeutic Potential of Exosomes in Soft Tissue Repair and Regeneration

Wan

Hussain

Behfar

et al. 2022

IJMS

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Soft tissue defects are common following trauma and tumor extirpation. These injuries can result in poor functional recovery and lead to a diminished quality of life. The healing of skin and muscle is a complex process that, at present, leads to incomplete recovery and scarring. Regenerative medicine may offer the opportunity to improve the healing process and functional outcomes. Barriers to regenerative strategies have included cost, regulatory hurdles, and the need for cell-based therapies. In recent years, exosomes, or extracellular vesicles, have gained tremendous attention in the field of soft tissue repair and regeneration. These nanosized extracellular particles (30–140 nm) can break the cellular boundaries, as well as facilitate intracellular signal delivery in various regenerative physiologic and pathologic processes. Existing studies have established the potential of exosomes in regenerating tendons, skeletal muscles, and peripheral nerves through different mechanisms, including promoting myogenesis, increasing tenocyte differentiation and enhancing neurite outgrowth, and the proliferation of Schwann cells. These exosomes can be stored for immediate use in the operating room, and can be produced cost efficiently. In this article, we critically review the current advances of exosomes in soft tissue (tendons, skeletal muscles, and peripheral nerves) healing. Additionally, new directions for clinical applications in the future will be discussed.

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“…Skeletal muscle is a remarkable organ with intrinsic plasticity because of its regenerative and reparative capabilities in response to exercise, traumatic injury, and disease. The ability to respond to these stimuli require tunable changes in gene expression often under the control of noncoding RNAs ( Chen et al, 2020 ; Aránega et al, 2021 ; Archacka et al, 2021 ; La Rocca et al, 2021 ). A subset of miRNAs have been identified and shown to be muscle-enriched, play key functional roles in myogenesis and muscle function, and are referred to as myomiRs ( Horak et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

miR-486 is essential for muscle function and suppresses a dystrophic transcriptome

et al. 2022

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miR-486 is a muscle-enriched microRNA, or “myomiR,” that has reduced expression correlated with Duchenne muscular dystrophy (DMD). To determine the function of miR-486 in normal and dystrophin-deficient muscles and elucidate miR-486 target transcripts in skeletal muscle, we characterized mir-486 knockout mice (mir-486 KO). mir-486 KO mice developed disrupted myofiber architecture, decreased myofiber size, decreased locomotor activity, increased cardiac fibrosis, and metabolic defects were exacerbated in mir-486 KO:mdx5cv (DKO) mice. To identify direct in vivo miR-486 muscle target transcripts, we integrated RNA sequencing and chimeric miRNA eCLIP sequencing to identify key transcripts and pathways that contribute towards mir-486 KO and dystrophic disease pathologies. These targets included known and novel muscle metabolic and dystrophic structural remodeling factors of muscle and skeletal muscle contractile transcript targets. Together, our studies identify miR-486 as essential for normal muscle function, a driver of pathological remodeling in dystrophin-deficient muscle, a useful biomarker for dystrophic disease progression, and highlight the use of multiple omic platforms to identify in vivo microRNA target transcripts.

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Non-Coding RNAs as Regulators of Myogenesis and Postexercise Muscle Regeneration

Cited by 9 publications

References 145 publications

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

The Therapeutic Potential of Exosomes in Soft Tissue Repair and Regeneration

miR-486 is essential for muscle function and suppresses a dystrophic transcriptome

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