Non-apoptotic caspase activation sustains ovarian somatic stem cell functions by modulating Hedgehog-signalling and autophagy

Galasso, Alessia; Iakovleva, Daria; Baena‐López, Luis Alberto

doi:10.1101/722330

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…As for immunohistochemistry, wing discs were dissected in PBS and fixed in 4% formaldehyde in PBS for 20 min. In situ detection of fragmented genomic DNA was then performed according to methods established previously ( Galasso et al, 2020 ), using the DeadEnd Colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega).…”

Section: Methodsmentioning

confidence: 99%

Non-apoptotic activation of Drosophila caspase-2/9 modulates JNK signaling, the tumor microenvironment, and growth of wound-like tumors

Wang

Yamada

et al. 2022

Cell Reports

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Section: Methodsmentioning

confidence: 99%

Non-apoptotic activation of Drosophila caspase-2/9 modulates JNK signaling, the tumor microenvironment, and growth of wound-like tumors

Wang

Yamada

et al. 2022

Cell Reports

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“…Recent research paints a complex picture of caspase activity, outside their established apoptotic and inflammatory roles, with caspase activity being involved in a wide range of other fundamental cellular processes, including development, aging, proliferation, differentiation, and neuronal plasticity [50][51][52][53][54][55][56]. The collection of non-apoptotic caspase functions have been termed caspase-dependent non-lethal cellular processes (CDPs) [44].…”

Section: Death Receptorsmentioning

confidence: 99%

The Duality of Caspases in Cancer, as Told through the Fly

Hounsell

Fan

2021

IJMS

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Caspases, a family of cysteine-aspartic proteases, have an established role as critical components in the activation and initiation of apoptosis. Alongside this a variety of non-apoptotic caspase functions in proliferation, differentiation, cellular plasticity and cell migration have been reported. The activity level and context are important factors in determining caspase function. As a consequence of their critical role in apoptosis and beyond, caspases are uniquely situated to have pathological roles, including in cancer. Altered caspase function is a common trait in a variety of cancers, with apoptotic evasion defined as a “hallmark of cancer”. However, the role that caspases play in cancer is much more complex, acting both to prevent and to promote tumourigenesis. This review focuses on the major findings in Drosophila on the dual role of caspases in tumourigenesis. This has major implications for cancer treatments, including chemotherapy and radiotherapy, with the activation of apoptosis being the end goal. However, such treatments may inadvertently have adverse effects on promoting tumour progression and acerbating the cancer. A comprehensive understanding of the dual role of caspases will aid in the development of successful cancer therapeutic approaches.

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“…As for immunohistochemistry, wing discs were dissected in ice-cold PBS and fixed in 4% formaldehyde in PBS for 20 minutes. In situ detection of fragmented genomic DNA was then performed according to methods established previously (Galasso et al, 2020), using the DeadEnd Colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega).…”

Section: Tunel Stainingmentioning

confidence: 99%

Widespread non-apoptotic activation ofDrosophilaCaspase-2/9 limits JNK signaling, macrophage proliferation, and growth of wound-like tumors

Yamada

Baena‐López

2020

Preprint

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SummaryResistance to apoptosis due to caspase deregulation is considered one of the main hallmarks of cancer. However, the discovery of novel non-apoptotic caspase functions has revealed unknown intricacies about the interplay between these enzymes and tumor progression. To investigate this biological problem, we capitalized on a Drosophila tumor model highly relevant for humans that relies on the concomitant upregulation of EGFR and the JAK/STAT signaling pathway. Our results indicate that widespread non-apoptotic activation of initiator caspases limits JNK signaling and facilitates cell fate commitment in these tumors, thus preventing the overgrowth and exacerbation of malignant features. Intriguingly, these caspase functions are strongly linked to the ability of these enzymes to control the recruitment and subsequent proliferation in situ of macrophage-like cells on the tumor. These findings assign novel tumor-suppressor activities to caspases independent of apoptosis, while providing highly relevant molecular details to understanding their diverse contribution during tumor progression.

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Non-apoptotic caspase activation sustains ovarian somatic stem cell functions by modulating Hedgehog-signalling and autophagy

Cited by 4 publications

References 66 publications

Non-apoptotic activation of Drosophila caspase-2/9 modulates JNK signaling, the tumor microenvironment, and growth of wound-like tumors

Non-apoptotic activation of Drosophila caspase-2/9 modulates JNK signaling, the tumor microenvironment, and growth of wound-like tumors

The Duality of Caspases in Cancer, as Told through the Fly

Widespread non-apoptotic activation ofDrosophilaCaspase-2/9 limits JNK signaling, macrophage proliferation, and growth of wound-like tumors

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