Nodule-Specific Regulation of Phosphatidylinositol Transfer Protein Expression in Lotus japonicus

Kapranov, Philipp; Routt, Sheri M.; Bankaitis, Vytas A.; Bruijn, Frans J. de; Szczygłowski, Krzysztof

doi:10.1105/tpc.13.6.1369

Cited by 57 publications

(33 citation statements)

References 42 publications

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“…, 2000), has recently been identified as a member of a novel class of plant phosphatidylinositol transfer protein (PITP)‐like proteins (Böhme et al. , 2004; Kapranov et al. , 2001) and is not discussed further here.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the root‐hair morphogenesis transcriptome reveals the molecular identity of six genes with roles in root‐hair development in Arabidopsis

Jones

Raymond

Smirnoff³

2005

The Plant Journal

191

171

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SummaryRoot-hair morphogenesis is a model for studying the genetic regulation of plant cell development, and doublemutant analyses have revealed a complex genetic network underlying the development of this type of cell. Therefore, to increase knowledge of gene expression in root hairs and to identify new genes involved in roothair morphogenesis, the transcriptomes of the root-hair differentiation zone of wild-type (WT) plants and a tipgrowth defective root-hair mutant, rhd2-1, were compared using Affymetrix ATH1 GeneChips Ò . A set of 606 genes with significantly greater expression in WT plants defines the 'root-hair morphogenesis transcriptome'. Compared with the whole genome, this set is highly enriched in genes known to be involved in root-hair morphogenesis. The additional gene families and functional groups enriched in the root-hair morphogenesis transcriptome are cell wall enzymes, hydroxyproline-rich glycoproteins (extensins) and arabinogalactan proteins, peroxidases, receptor-like kinases and proteins with predicted glycosylphosphatidylinositol (GPI) anchors. To discover new root-hair genes, 159 T-DNA insertion lines identified from the root-hair morphogenesis transcriptome were screened for defects in root-hair morphogenesis. This identified knockout mutations in six genes (RHM1-RHM6) that affected root-hair morphogenesis and that had not previously been identified at the molecular level: At2g03720 (similar to Escherichia coli universal stress protein); At3g54870 (armadillo-repeat containing kinesin-related protein); At4g18640 (leucine-rich repeat receptor-like kinase subfamily VI); At4g26690 (glycerophosphoryl diester phosphodiesterase-like GPI-anchored protein); At5g49270 (COBL9 GPI-anchored protein) and At5g65090 (inositol-1,4,5 triphosphate 5-phosphatase-like protein). The mutants were transcript null, their root-hair phenotypes were characterized and complementation testing with uncloned root-hair genes was performed. The results suggest a role for GPI-anchored proteins and lipid rafts in root-hair tip growth because two of these genes (At4g26690 and At5g49270) encode predicted GPI-anchored proteins likely to be associated with lipid rafts, and several other genes previously shown to be required for root-hair development also encode proteins associated with sterol-rich lipid rafts.

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Section: Resultsmentioning

confidence: 99%

Analysis of the root‐hair morphogenesis transcriptome reveals the molecular identity of six genes with roles in root‐hair development in Arabidopsis

Jones

Raymond

Smirnoff³

2005

The Plant Journal

191

171

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“…These proteins include the Ssh1p and Ssh2p polypeptides from soybean, AtSEC14 from Arabidopsis and the LjPLPs from Lotus japonicus (Jouannic et al. , 1998; Kapranov et al. , 2001; Kearns et al.…”

Section: Introductionmentioning

confidence: 99%

“…, 1998). Moreover LjPLPIV expression from L. japonicus is specifically controlled in nodules (Kapranov et al. , 2001).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis COW1 gene encodes a phosphatidylinositol transfer protein essential for root hair tip growth

et al. 2004

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SummaryRoot hairs are a major site for the uptake of water and nutrients into plants, and they form an increasingly important model system for the study of development in higher plants. We now report on the molecular genetic analysis of the srh1 mutant in Arabidopsis thaliana impaired in root hair tip growth. We show that srh1 is a new allele of cow1 (can of worms1) and we identified the COW1 gene using a positional cloning strategy. The N-terminus of the COW1 protein is 32% identical to an essential phosphatidylinositol transfer protein (PITP), the yeast Sec14 protein (sec14p) while the C-terminus is 34.5% identical to a late nodulin of Lotus japonicus, Nlj16. We show that expression of the COW1 lipid-binding domain complements the growth defect associated with Sec14p dysfunction in yeast. In addition, we show that GFP fused to the COW1 protein specifically accumulates at the site of root hair outgrowth. We conclude that the COW1 protein is a PITP, essential for proper root hair growth.

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“…The Sec14 domain is also associated with uncharacterized modules that include nodulin domains (in higher plants) (Vincent et al 2005; Kapranov et al 2001), Golgi dynamics (GOLD) domains in metazoans and higher plants (Anantharaman and Aravind 2002), metazoan-specific PRELI domains (thought to function as mitochondrial targeting motifs) (Dee and Moffat 2005), and a GTPase motif of uncertain function (Habermehl et al 2004). Stand-alone Sec14 domain proteins are more prevalent in simpler eukaryotes.…”

Section: 5 the Sec14 Superfamily Of Pitpsmentioning

confidence: 99%

“…These units present basic carboxy-terminal tails (Vincent et al 2005; Kapranov et al 2001), thereby resembling known PIP-binding motifs that operate via membrane surface electrostatics (e.g. the MARCKS peptide (McLaughlin and Murray 2005).…”

Section: 9 Definition Of Sensing Territoriesmentioning

confidence: 99%

Sec14 Like PITPs Couple Lipid Metabolism with Phosphoinositide Synthesis to Regulate Golgi Functionality

Mousley

Davison

Bankaitis

2012

Subcellular Biochemistry

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An interface coordinating lipid metabolism with proteins that regulate membrane trafficking is necessary to regulate Golgi morphology and dynamics. Such an interface facilitates the membrane deformations required for vesicularization, forms platforms for protein recruitment and assembly on appropriate sites on a membrane surface and provides lipid co-factors for optimal protein activity in the proper spatio-temporally regulated manner. Importantly, Sec14 and Sec14-like proteins are a unique superfamily of proteins that sense specific aspects of lipid metabolism, employing this information to potentiate phosphoinositide production. Therefore, Sec14 and Sec14 like proteins form central conduits to integrate multiple aspects of lipid metabolism with productive phosphoinositide signaling.

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Nodule-Specific Regulation of Phosphatidylinositol Transfer Protein Expression in Lotus japonicus

Cited by 57 publications

References 42 publications

Analysis of the root‐hair morphogenesis transcriptome reveals the molecular identity of six genes with roles in root‐hair development in Arabidopsis

Analysis of the root‐hair morphogenesis transcriptome reveals the molecular identity of six genes with roles in root‐hair development in Arabidopsis

The Arabidopsis COW1 gene encodes a phosphatidylinositol transfer protein essential for root hair tip growth

Sec14 Like PITPs Couple Lipid Metabolism with Phosphoinositide Synthesis to Regulate Golgi Functionality

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