Nod factors activate both heterotrimeric and monomeric G-proteins in Vigna unguiculata (L.) Walp

Kelly, Marilyn N.; Irving, Helen Ruth

doi:10.1007/s00425-002-0900-8

Cited by 22 publications

(10 citation statements)

References 43 publications

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“…For example, nodules show higher expression of GmGa1-3, but not GmGa4. This is interesting because multiple pharmacological experiments suggest the involvement of G-proteins in signaling processes related to nodulation (Pingret et al, 1998;den Hartog et al, 2001;Kelly & Irving, 2003;Charron et al, 2004;Sun et al, 2007;Santos-Briones et al, 2009).…”

Section: Developmental Regulation Of Soybean Heterotrimeric G-proteinmentioning

confidence: 99%

“…Expanding on studies of model monocots and dicots, growing evidence suggests that G-protein signaling plays a critical role in a range of economically important legume crops during normal growth and development as well as during symbiosis. Some examples include the G-proteinregulated blue and red light signaling pathways in pea (Warpeha et al, 1991), induction of root hair deformation in Vigna, and expression of nodulation-specific genes in Medicago by mastoparan, a known G-protein agonist (Kelly & Irving, 2003;Sun et al, 2007). Additionally, some wellestablished effectors of G-protein signaling, for example phospholipase C (PLC) and D (PLD) are involved in mastoparan-induced root hair deformation and development of root nodules (Pingret et al, 1998;den Hartog et al, 2001;Charron et al, 2004;Santos-Briones et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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An elaborate heterotrimeric G‐protein family from soybean expands the diversity of plant G‐protein networks

2010

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Summary• The repertoire of heterotrimeric G-proteins in plant species analyzed thus far is simple, with the presence of only two possible canonical heterotrimers in Arabidopsis and rice vs hundreds in animal systems. We assessed whether genome duplication events have resulted in the multiplicity of G-protein in plant species like soybean that would increase the complexity of G-protein networks.• We identified and amplified four Ga, four Gb and two Gc proteins, analyzed their expression profile by quantitative PCR during different developmental stages. We purified the four Ga proteins and analyzed their guanosine-5¢-triphosphate (GTP)-binding and GTPase activity. We performed yeast-based interaction analysis to assess the interaction specificity of different G-protein subunits.• Our results show that all 10 G-protein genes are retained in the soybean genome and ubiquitously expressed. The four Ga proteins seem to be plasma membrane-localized. The G-protein genes have interesting expression profiles during seed development and germination. The four Ga proteins form two distinct groups based on their GTPase activity. Yeast-based interaction analyses predict that the proteins interact in most of the possible combinations, with some degree of interaction specificity between duplicated gene pairs.• This research identifies the most elaborate heterotrimeric G-protein network known to date in the plant kingdom.

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Section: Developmental Regulation Of Soybean Heterotrimeric G-proteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An elaborate heterotrimeric G‐protein family from soybean expands the diversity of plant G‐protein networks

2010

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“…Work with mutants has implicated G proteins in ion-channel regulation, control of seed germination, light responses (Okamoto et al, 2001, but, see also Jones et al, 2003), cell division and elongation, and responses to the phytohormones abscisic acid (ABA), gibberellic acid (GA) and auxin (Ma, 1994;Fujisawa et al, 2001;Jones, 2002). Pharmacological studies have also implicated heterotrimeric G proteins in plant interactions with symbiotic bacteria (Pingret et al, 1998;Kelly & Irving, 2003). For example, S1P, lysophosphatidic acid and S1P is used as a signal in G-protein-based pathways in guard cells metabolites eicosanoids (Ng et al, 2001;Coursol et al, 2003)…”

Section: G-protein-signalling Genes In the Plant Genomementioning

confidence: 99%

Plants: the latest model system for G‐protein research

2004

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In humans, heterotrimeric G proteins couple stimulus perception by G-protein-coupled receptors (GPCRs) with numerous downstream effectors. By contrast, despite great complexity in their signal-transduction attributes, plants have a simpler repertoire of G-signalling components. Nonetheless, recent studies on Arabidopsis thaliana have shown the importance of plant G-protein signalling in such fundamental processes as cell proliferation, hormone perception and ion-channel regulation.

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“…Plant G-proteins are also involved in numerous signaling processes including interactions with rhizobia [23], defense against pathogens [24], [25], [26], [27], [28], [29], [30], morphological development and growth [31], [32], [33], cell proliferation [34], [35], ion-channel regulation [36], stomatal control [37], [38], light perception [32], [39], [40], [41], [42], abiotic stress [43], [44], [45], [46] and hormonal responses including glucose, brassinosteroid, abscisic acid and jasmonate [47], [48], [49], [50], [51], [52], [53]. G-proteins have also been linked to yield related quantitative trait loci in important crops such as rice [54], [55].…”

Section: Introductionmentioning

confidence: 99%

Signaling Specificity Provided by the Arabidopsis thaliana Heterotrimeric G-Protein γ Subunits AGG1 and AGG2 Is Partially but Not Exclusively Provided through Transcriptional Regulation

et al. 2013

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The heterotrimeric G-protein complex in Arabidopsis thaliana consists of one α, one ß and three γ subunits. While two of the γ subunits, AGG1 and AGG2 have been shown to provide functional selectivity to the Gßγ dimer in Arabidopsis, it is unclear if such selectivity is embedded in their molecular structures or conferred by the different expression patterns observed in both subunits. In order to study the molecular basis for such selectivity we tested genetic complementation of AGG1- and AGG2 driven by the respectively swapped gene promoters. When expressed in the same tissues as AGG1, AGG2 rescues some agg1 mutant phenotypes such as the hypersensitivity to Fusarium oxysporum and D-mannitol as well as the altered levels of lateral roots, but does not rescue the early flowering phenotype. Similarly, AGG1 when expressed in the same tissues as AGG2 rescues the osmotic stress and lateral-root phenotypes observed in agg2 mutants but failed to rescue the heat-stress induction of flowering. The fact that AGG1 and AGG2 are functionally interchangeable in some pathways implies that, at least for those pathways, signaling specificity resides in the distinctive spatiotemporal expression patterns exhibited by each γ subunit. On the other hand, the lack of complementation for some phenotypes indicates that there are pathways in which signaling specificity is provided by differences in the primary AGG1 and AGG2 amino acid sequences.

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Nod factors activate both heterotrimeric and monomeric G-proteins in Vigna unguiculata (L.) Walp

Cited by 22 publications

References 43 publications

An elaborate heterotrimeric G‐protein family from soybean expands the diversity of plant G‐protein networks

An elaborate heterotrimeric G‐protein family from soybean expands the diversity of plant G‐protein networks

Plants: the latest model system for G‐protein research

Signaling Specificity Provided by the Arabidopsis thaliana Heterotrimeric G-Protein γ Subunits AGG1 and AGG2 Is Partially but Not Exclusively Provided through Transcriptional Regulation

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