NMR three-dimensional structure of the cationic peptide Stigmurin from Tityus stigmurus scorpion venom: In vitro antioxidant and in vivo antibacterial and healing activity

Daniele-Silva, Alessandra; Rodrigues, Suedson de Carvalho Silva; Santos, Elizabeth Cristina Gomes dos; Neto, Moacir Fernandes de Queiroz; Rocha, Hugo Alexandre de Oliveira; Silva-Júnior, Arnóbio Antônio da; Resende, Jarbas M.; Araújo, Renata Mendonça; Fernandes-Pedrosa, Matheus de Freitas

doi:10.1016/j.peptides.2020.170478

Cited by 11 publications

(7 citation statements)

References 92 publications

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“…The development of multifunctional molecules, including natural products, capable of simultaneously combating several pathological characteristics, acting as chelating agents, antioxidants, anti-inflammatories, and peptide aggregation reducers, among others, is considered a new perspective for the treatment of neurodegenerative diseases ( Sales et al, 2019 ). In this context, components present in the scorpion venom have demonstrated anti-inflammatory ( Veloso Júnior et al, 2019 ), chelating ( Melo et al, 2017 ), and antioxidant action ( Daniele-Silva et al, 2021 ), constituting a promising source for the development of new drugs.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of molecules with antioxidant effects in scorpions has been reported in the literature ( Wali et al, 2020 ). The Stigmurin (FFSLIPSLVGGLISAFK-NH2), cationic peptide of T. stigmurus , showed hydroxyl radical scavenging above 70% at 10 μM ( Daniele-Silva et al, 2021 ). A peptide fraction isolated from the venom of Buthus occitanus has been demonstrated to exhibit the antioxidant and free radical scavenger effects ( Bekheet et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, some researchers have demonstrated the multifunctionality of linear peptides present in scorpions ( D’Suze et al, 2010 ; Gao et al, 2010 ; Guo et al, 2013 ; Ortiz et al, 2015 ; Daniele-Silva et al, 2016 , 2021 ; Guilhelmelli et al, 2016 ; Ojeda et al, 2016 ; Cesa-Luna et al, 2019 ), and their antibacterial, antifungal, hypotensive, anticancer, and immunomodulatory activities have been identified ( Zeng et al, 2012 ; Almaaytah et al, 2013 ; Daniele-Silva et al, 2016 , 2021 ; Machado et al, 2016 ; Torres-Rêgo et al, 2019 ; Furtado et al, 2020 ). However, little researches about anionic peptides of these arachnids have been reported.…”

Section: Introductionmentioning

confidence: 99%

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TanP: A Multifunctional Anionic Peptide From Tityus stigmurus Scorpion Venom

Melo

Oliveira²,

Neto³

et al. 2022

Front. Mol. Biosci.

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Anionic peptides of scorpions are molecules rich in aspartic and/or glutamic acid residues and correspond to a class of peptides without disulfide bonds that are still little explored. TanP is a linear anionic peptide (50 amino acid residues and net charge −20) present in the venom gland of the scorpion, Tityus stigmurus, with chelating properties for Cu2+ ion and immunomodulatory properties. The therapeutic application of chelating molecules is related to cases of acute or chronic intoxication by metals, neurodegenerative diseases, hematological diseases, healing of skin wounds, cardiovascular diseases, and cancer. In this approach, the chelating activity of TanP was evaluated in relation to new metal ions (Fe2+ and Zn2+) of biological importance, as well as its antioxidant, hemostatic, immunomodulatory, and healing potential, aiming to expand the biological and biotechnological potential of this peptide. TanP (25 µM) was able to form stable complexes with Fe2+ in a ratio of 1:5 (TanP: Fe2+). Theoretical results suggest that TanP can work as a sensor to identify and quantify Fe2+ ions. The fluorescence intensity of TanP (1.12 µM) decreased significantly after the addition of Fe2+, obtaining the highest ratio 1: 7.4 (TanP: Fe2+) that led to the lowest fluorescence intensity. For Zn2+, no relevant spectral change was noted. TanP (50 µM) showed a maximum of 3% of hemolytic activity, demonstrating biocompatibility, as well as exhibiting a 1,1-diphenyl-2-picrylhydrazyl radical–scavenging activity of above 70% at all the concentrations tested (1–25 μM), and 89.7% iron-chelating activity at 25 μM and 96% hydroxyl radical–scavenging activity at 73.6 μM. In addition, TanP (12.5 and 25 µM) revealed an anticoagulant effect, prolonging the clotting time in prothrombin time and activated partial thromboplastin time assays, with no fibrinogenolytic activity. TanP (12.5 and 25 µM) induced the release of TNF-α by murine macrophages, in the absence of lipopolysaccharides, with a concentration-dependent increase and also stimulated the migration of 3T3 cells in the in vitro healing assay. Thus, TanP revealed a multifunctional potential, being useful as a prototype for the development of new therapeutic and biotechnological agents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TanP: A Multifunctional Anionic Peptide From Tityus stigmurus Scorpion Venom

Melo

Oliveira²,

Neto³

et al. 2022

Front. Mol. Biosci.

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“…The animals were topically treated with daily 100 μL sterile saline, povidone-iodine solution (PVP-I), COS1, or COS5 (30 and 300 mg/kg) for 7 days. Photo documentation using the ImageJ 1.52a program software (National Institutes of Health, MD, USA) was performed daily to assess tissue repair and the lesion extent was measured from the first to the seventh day [ 73 , 74 ]. The size on the initial day of the experiment was considered as 100% (0% reduction).…”

Section: Methodsmentioning

confidence: 99%

Production and Characterization of Chitooligosaccharides: Evaluation of Acute Toxicity, Healing, and Anti-Inflammatory Actions

Andrade

Araújo

Torres-Rêgo

et al. 2021

IJMS

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The search for promising biomolecules such as chitooligosaccharides (COS) has increased due to the need for healing products that act efficiently, avoiding complications resulting from exacerbated inflammation. Therefore, this study aimed to produce COS in two stages of hydrolysis using chitosanases derived from Bacillus toyonensis. Additionally, this study aimed to structurally characterize the COS via mass spectrometry, to analyze their biocompatibility in acute toxicity models in vivo, to evaluate their healing action in a cell migration model in vitro, to analyze the anti-inflammatory activity in in vivo models of xylol-induced ear edema and zymosan-induced air pouch, and to assess the wound repair action in vivo. The structural characterization process pointed out the presence of hexamers. The in vitro and in vivo biocompatibility of COS was reaffirmed. The COS stimulated the fibroblast migration. In the in vivo inflammatory assays, COS showed an antiedematogenic response and significant reductions in leukocyte migration, cytokine release, and protein exudate. The COS healing effect in vivo was confirmed by the significant wound reduction after seven days of the experiment. These results indicated that the presence of hexamers influences the COS biological properties, which have potential uses in the pharmaceutical field due to their healing and anti-inflammatory action.

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“…It is possible to observe the amphipathic character of these structures through the hydrophobic and hydrophilic regions well distributed along the peptide chain. It is noticeable that Stigmurin exhibits greater hydrophobicity than IsCT ( Dai et al, 2001 ; Daniele-Silva et al, 2021 ).…”

Section: Physicochemical Properties That Influence the Antimicrobial ...mentioning

confidence: 99%

Antimicrobial Peptide Analogs From Scorpions: Modifications and Structure-Activity

Amorim-Carmo

Parente

Souza

et al. 2022

Front. Mol. Biosci.

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The rapid development of multidrug-resistant pathogens against conventional antibiotics is a global public health problem. The irrational use of antibiotics has promoted therapeutic limitations against different infections, making research of new molecules that can be applied to treat infections necessary. Antimicrobial peptides (AMPs) are a class of promising antibiotic molecules as they present broad action spectrum, potent activity, and do not easily induce resistance. Several AMPs from scorpion venoms have been described as a potential source for the development of new drugs; however, some limitations to their application are also observed. Here, we describe strategies used in several approaches to optimize scorpion AMPs, addressing their primary sequence, biotechnological potential, and characteristics that should be considered when developing an AMP derived from scorpion venoms. In addition, this review may contribute towards improving the understanding of rationally designing new molecules, targeting functional AMPs that may have a therapeutic application.

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NMR three-dimensional structure of the cationic peptide Stigmurin from Tityus stigmurus scorpion venom: In vitro antioxidant and in vivo antibacterial and healing activity

Cited by 11 publications

References 92 publications

TanP: A Multifunctional Anionic Peptide From Tityus stigmurus Scorpion Venom

TanP: A Multifunctional Anionic Peptide From Tityus stigmurus Scorpion Venom

Production and Characterization of Chitooligosaccharides: Evaluation of Acute Toxicity, Healing, and Anti-Inflammatory Actions

Antimicrobial Peptide Analogs From Scorpions: Modifications and Structure-Activity

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