NMR Studies of Body Fluids and Tissue Extracts

Bell, Jimmy D.; Preece, Nicholas E.; Parkes, Harold G.

doi:10.1016/b978-0-12-283980-1.50019-3

Cited by 8 publications

(6 citation statements)

References 70 publications

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“…Traditionally, tissue analysis required sample extraction prior to analysis by conventional liquid-state NMR spectroscopy. This has highlighted interesting differences between different types of tissues, 14 however, extraction processes can be selective (e.g., in the case of aqueous or perchlorate extracts) leading to loss of certain tissue components such as lipids or proteins. Alternatively, in vivo approaches such as MRS on intact tissues are prone to poor sensitivity and signal dispersion based on the heterogeneity of the sample and, typically, the low magnetic fields employed.…”

Section: Spectroscopic Techniquesmentioning

confidence: 99%

Analytical Strategies in Metabonomics

2006

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To perform metabonomics investigations, it is necessary to generate comprehensive metabolite profiles for complex samples such as biofluids and tissue/tissue extracts. Analytical technologies that can be used to achieve this aim are constantly evolving, and new developments are changing the way in which such profiles' metabolite profiles can be generated. Here, the utility of various analytical techniques for global metabolite profiling, such as, e.g., 1H NMR, MS, HPLC-MS, and GC-MS, are explored and compared.

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Section: Spectroscopic Techniquesmentioning

confidence: 99%

Analytical Strategies in Metabonomics

2006

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“…The other peaks were assigned to adenosine monophosphate (peak 9), fructose-6-phosphate (peak 11), and glycerol phosphate (peak 12) from published results (7). Resonance at 6.94 ppm was not assigned.…”

Section: In Vitro ( 31 P)mentioning

confidence: 99%

In vivo and in vitro MR spectroscopic profile of central neurocytomas

Jayasundar

Shah

Vaishya

et al. 2003

Magnetic Resonance Imaging

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This study reports in vivo and in vitro magnetic resonance spectroscopic findings in two cases of central neurocytomas (CNC) confirmed by immunohistochemistry. Volume localized in vivo proton magnetic resonance spectroscopy (MRS) was carried out before surgery using a point resolved spectroscopy (PRESS) sequence with a repetition time of six seconds and an echo time of 135 msec. Normal spectrum was obtained from gray matter from a volunteer for comparison. 1 H and 31 P in vitro MRS studies were carried out at 9.4 T on the extracts prepared from the surgically excised tumors. The in vivo spectra showed prominent glycine (Gly) and choline (Cho) and low N-acetyl aspartate compared to the normal. The Gly peak was assigned using the in vitro studies. These studies showed that the major contribution to the Cho peak observed in vivo is from phosphocholine. A combination of the presence of NAA and an increased Gly in the proton MR spectrum could be a characteristic feature of CNCs, which are rare intraventricular tumors of neuronal origin.

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“…Cross-relaxation has been discussed (5). NMR of body fluids and tissue extracts has been discussed (6), as has its application to cell metabolism (7). An exciting field is the use of NMR in conjunction with other experiments to correlate information.…”

Section: Reviews and Booksmentioning

confidence: 99%