NMR insights on nano silver post-surgical treatment of superficial caseous lymphadenitis in small ruminants

Stanisić, Danijela; Fregonesi, Natália L.; Barros, Caio; Pontes, João G. M.; Fulaz, Stephanie; Menezes, Ulisses José; Nicoleti, Jorge L.; Castro, Thiago Luiz de Paula; Seyffert, Núbia; Azevedo, Vasco; Portela, Ricardo Wagner; Tasić, Ljubica

doi:10.1039/c8ra08218a

Cited by 9 publications

(9 citation statements)

References 53 publications

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“…The solution was maintained at 28 • C in flasks sealed with aluminum foil until the formation of the nanoparticles. The characterization of the Fusarium oxysporum-AgNPs was performed using UV-Vis, showing a characteristic maximum absorbance at 440 nm (Supplementary Figure S1), dynamic light scattering and transmission electron microscopy, as previously described [11,23] and showed in Table 1 and Supplementary Figure S2. The AgNP were spherical, showing a size of 28.0 ± 13.1 nm, a polydispersity of 0.231, and a zeta potential of −31.7 ± 2.8 mV (Figure S1) [11,23].…”

Section: Synthesis Of Silver Nanoparticlesmentioning

confidence: 95%

“…According to the M27-S4 document [27], the breakpoint for fluconazole was considered for the classification of the fungal isolates according to the corresponding MIC100 (µg/mL), as follows: resistant (R) ≥ 8; dose-dependent susceptible (SDD) = 4; susceptible (S) ≤ 2, except for C. glabrata, where the interpretative endpoints were SDD ≤ 32 and R ≥ 64. Breakpoints for ketoconazole, nystatin and AmB were not available in M27-S4 document [23], and this classification was not performed for these drugs.…”

Section: Broth Microdilution Assaymentioning

confidence: 99%

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Activity of Fusarium oxysporum-Based Silver Nanoparticles on Candida spp. Oral Isolates

et al. 2022

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Candida spp. resistant to commercially available antifungals are often isolated from patients with oral candidiasis, a situation that points to the need for the development of new therapies. Thus, we evaluated the activity of Fusarium oxysporum-based silver nanoparticles (AgNPs) on Candida spp. isolated from denture stomatitis lesions. Candida isolates were molecularly identified and submitted to susceptibility assays using AgNPs and commercial fungicides. The interference on biofilm formation and the mechanisms of action of AgNPs on Candida spp. were also investigated. Scanning electron microscopy was used to evaluate the morphology of AgNP-treated Candida. Candida albicans was the most frequent species isolated from denture stomatitis cases. All Candida spp. were susceptible to AgNPs at low concentrations, except Candida parapsilosis. AgNPs caused surface damage, cell disruption, and biofilm formation inhibition. The ergosterol supplementation protected C. albicans against the AgNP action. AgNPs are effective against Candida spp. and can be faced as a promising new therapeutic agent against oral candidiasis.

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Section: Synthesis Of Silver Nanoparticlesmentioning

confidence: 95%

Section: Broth Microdilution Assaymentioning

confidence: 99%

Activity of Fusarium oxysporum-Based Silver Nanoparticles on Candida spp. Oral Isolates

et al. 2022

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“…The solution was maintained at 28°C and sealed with aluminum foil until the formation of the nanoparticles. The AgNPs were characterized and showed sizes of 28.0 ± 13.1 nm, polydispersity of 0.231, zeta potential of −31.7 ± 2.8 mV, and were spherical in form (Stanisic et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…For the post-surgical treatment of CL, an ointment based on AgNPs, natural waxes, and oils was made, as described by Stanisic et al (2018). In the first step of ointment production, the oils and waxes (31% solid vaseline, 20% lanoline, 10% liquid vaseline, 9.0% cera alba, 8.0% cetostearyl alcohol, and 2.0% cholesterol) were blended and heated at 60°C until the mixture was homogeneous, and all of the ingredients were fused.…”

Section: Methodsmentioning

confidence: 99%

“…Then, the emulsion was cooled to room temperature (25°C), while constantly mixing to maintain its homogeneity. In the second step of ointment production, 20% part-to-part of a 12.8 mg/mL colloidal solution of biogenic AgNPs was added gradually to the mixture at room temperature under constant stirring (Stanisic et al, 2018). The final ointment was a gray oil/water cream that was easy to apply and left an oily film when applied to the skin surface.…”

Section: Methodsmentioning

confidence: 99%

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Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants

et al. 2019

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Caseous lymphadenitis (CL) is an infectious and zoonotic disease characterized by the development of granulomas in the lymph nodes and internal organs of small ruminants. The etiological agent of this disease is Corynebacterium pseudotuberculosis , a Gram-positive and facultative intracellular bacterium. The conventional treatment for CL consists of drainage and chemical cauterization of the lesions using a 10% iodine solution. However, this type of treatment is not effective, due to iodine’s cytotoxic profile and low antibacterial activity. Currently, silver nanoparticles (AgNPs) can be seen as an alternative treatment for CL due to their antimicrobial activity and wound healing effects. Therefore, the present study aimed to evaluate AgNPs as a post-surgical treatment for CL. Twenty-nine goats and sheep with clinical signs of CL were selected. Surgical intervention was performed to excise the caseous lesions. To treat the lesions, an ointment formulation based on AgNP mixed with natural waxes and oils was used in the experimental group, and the conventional treatment with 10% iodine was used in the control group. Bacteria were isolated from the excised caseous material. The animals were observed for 8 weeks after the surgical treatment, and blood samples were taken weekly. The surgical wounds of sheep treated with AgNP healed faster, and the surgical wound area was smaller during the observation period; the latter effect was also observed in goats. AgNP-treated animals also had less purulent discharge and less moisture in the surgical wounds. The AgNP-treated animals had lower leukocyte counts and lower titers of anti- C. pseudotuberculosis antibodies. There was no statistically significant difference between the groups with regard to the hemogram results. The results of the susceptibility testing of C. pseudotuberculosis strains (T1, 1002, FRC41, and VD57 strains) and clinical isolates to AgNPs showed growth inhibition, even at low concentrations. It can be concluded that post-surgical treatment of CL using the AgNP-based ointment may be a promising tool in the control of CL, through faster healing, decreased wound contamination, and no apparent toxic effects.

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A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats

Nicoleti

Braga

Stanisić

et al. 2023

Appl Microbiol Biotechnol

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NMR insights on nano silver post-surgical treatment of superficial caseous lymphadenitis in small ruminants

Abstract: Biogenic nanosilver in a pharmaceutical cream for wound healing in animal and human healthcare.

Cited by 9 publications

References 53 publications

Activity of Fusarium oxysporum-Based Silver Nanoparticles on Candida spp. Oral Isolates

Activity of Fusarium oxysporum-Based Silver Nanoparticles on Candida spp. Oral Isolates

Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants

A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats

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