NMR and LCMS analytical platforms exhibited the nephroprotective effect of Clinacanthus nutans in cisplatin-induced nephrotoxicity in the in vitro condition

Mahmod, Ilya Iryani; Ismail, Intan Safinar; Alitheen, Noorjahan Banu Mohamed; Normi, Yahaya M.; Abas, Faridah; Khatib, Alfi; Rudiyanto, .; Latip, Jalifah

doi:10.1186/s12906-020-03067-3

Cited by 13 publications

(13 citation statements)

References 45 publications

(54 reference statements)

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“…Additionally, Gilda et al [18] identified 35 metabolites, along with antioxidant and AG inhibitory effects of the fruits of both Morus alba and Morus nigra. Similar technology has been applied to evaluate the activity of Clinacanthus nutans leaves against cisplatin toxicity [19]. In addition, potential thrombin/factor Xa inhibitors were reported from Salvia miltiorrhiza Bunge and Ligusticum chuanxiong Hort using the LC-MS-based multivariate data analysis technique [20,21].…”

Section: Introductionmentioning

confidence: 99%

Characterization of α-Glucosidase Inhibitors from Psychotria malayana Jack Leaves Extract Using LC-MS-Based Multivariate Data Analysis and In-Silico Molecular Docking

et al. 2020

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Psychotria malayana Jack has traditionally been used to treat diabetes. Despite its potential, the scientific proof in relation to this plant is still lacking. Thus, the present study aimed to investigate the α-glucosidase inhibitors in P.malayana leaf extracts using a metabolomics approach and to elucidate the ligand–protein interactions through in silico techniques. The plant leaves were extracted with methanol and water at five various ratios (100, 75, 50, 25 and 0% v/v; water–methanol). Each extract was tested for α-glucosidase inhibition, followed by analysis using liquid chromatography tandem to mass spectrometry. The data were further subjected to multivariate data analysis by means of an orthogonal partial least square in order to correlate the chemical profile and the bioactivity. The loading plots revealed that the m/z signals correspond to the activity of α-glucosidase inhibitors, which led to the identification of three putative bioactive compounds, namely 5′-hydroxymethyl-1′-(1, 2, 3, 9-tetrahydro-pyrrolo (2, 1-b) quinazolin-1-yl)-heptan-1′-one (1), α-terpinyl-β-glucoside (2), and machaeridiol-A (3). Molecular docking of the identified inhibitors was performed using Auto Dock Vina software against the crystal structure of Saccharomyces cerevisiae isomaltase (Protein Data Bank code: 3A4A). Four hydrogen bonds were detected in the docked complex, involving several residues, namely ASP352, ARG213, ARG442, GLU277, GLN279, HIE280, and GLU411. Compound 1, 2, and 3 showed binding affinity values of −8.3, −7.6, and −10.0 kcal/mol, respectively, which indicate the good binding ability of the compounds towards the enzyme when compared to that of quercetin, a known α-glucosidase inhibitor. The three identified compounds that showed potential binding affinity towards the enzymatic protein in molecular docking interactions could be the bioactive compounds associated with the traditional use of this plant.

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Section: Introductionmentioning

confidence: 99%

Characterization of α-Glucosidase Inhibitors from Psychotria malayana Jack Leaves Extract Using LC-MS-Based Multivariate Data Analysis and In-Silico Molecular Docking

et al. 2020

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“…C. nutans has also been evaluated for its renoprotective activities. The renoprotective effect of C. nutans has been demonstrated by several in-vivo and in-vitro studies [ 63 , 64 , 65 ]. In 2017, the nephroprotective effect of C. nutans leaves against cisplatin-induced nephrotoxicity and the safety assessment of C. nutans leaves has been demonstrated by [ 63 ].…”

Section: Pharmacological and Medicinal Propertiesmentioning

confidence: 99%

“…Collectively, these findings highlighted the potential use of C. nutans leaves extract in the management and treatment of cisplatin-induced nephrotoxicity. Meanwhile, [ 64 ] has also reported the nephroprotective effect of C. nutans in cisplatin-induced nephrotoxicity under an in-vitro condition using the Proton Nuclear Magnetic Resonance ( 1 H NMR) and Liquid Chromatography Mass Spectroscopy (LCMS) techniques coupled with multivariate data analysis to characterize the metabolic variations in intracellular metabolites and the compositional changes of the corresponding culture media in rat renal proximal tubular cells (NRK-52E). Investigations of this study have highlighted the altered pathways perturbed by cisplatin induced nephrotoxic on NRK-52E cells which involved changes in amino acid metabolism, lipid metabolism and glycolysis such that choline, creatinine, phosphocholine, valine, acetic acid, phenylalanine, leucine, glutamic acid, threonine, uridine and proline as the main metabolites which differentiated the cisplatin induced group of NRK-52E from control cells extract while the corresponding media exhibited lactic acid, glutamine, glutamic acid and glucose-1-phosphate as the varied metabolites.…”

Section: Pharmacological and Medicinal Propertiesmentioning

confidence: 99%

“…Investigations of this study have highlighted the altered pathways perturbed by cisplatin induced nephrotoxic on NRK-52E cells which involved changes in amino acid metabolism, lipid metabolism and glycolysis such that choline, creatinine, phosphocholine, valine, acetic acid, phenylalanine, leucine, glutamic acid, threonine, uridine and proline as the main metabolites which differentiated the cisplatin induced group of NRK-52E from control cells extract while the corresponding media exhibited lactic acid, glutamine, glutamic acid and glucose-1-phosphate as the varied metabolites. C. nutans aqueous leaves extract at 1000 μg/mL exhibited the highest potential for a nephroprotective effect against cisplatin toxicity on NRK-52E cell lines at 89% of viability where the protective effect of C. nutans aqueous leaves extract could be discerned by the changes in the metabolites such as choline, alanine and valine in the pre-treated samples with those of the cisplatin-induced group [ 64 ]. Moreover, the nephroprotective effect of C. nutans against cisplatin-induced nephrotoxic human kidney cells was also reported by the same group using 8 different solvent extracts from C. nutans leaves.…”

Section: Pharmacological and Medicinal Propertiesmentioning

confidence: 99%

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A Narrative Review on the Phytochemistry, Pharmacology and Therapeutic Potentials of Clinacanthus nutans (Burm. f.) Lindau Leaves as an Alternative Source of Future Medicine

et al. 2021

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The application of natural products and supplements has expanded tremendously over the past few decades. Clinacanthus nutans (C. nutans), which is affiliated to the Acanthaceae family, has recently caught the interest of researchers from the countries of subtropical Asia due to its medicinal uses in alternative treatment for skin infection conditions due to insect bites, microorganism infections and cancer, as well as for health well-being. A number of bioactive compounds from this plant’s extract, namely phenolic compounds, sulphur containing compounds, sulphur containing glycosides compounds, terpens-tripenoids, terpens-phytosterols and chlorophyll-related compounds possess high antioxidant activities. This literature search yielded about one hundred articles which were then further documented, including the valuable data and findings obtained from all accessible electronic searches and library databases. The promising pharmacological activities from C. nutans leaves extract, including antioxidant, anti-cancer, anti-viral, anti-bacterial, anti-fungal, anti-venom, analgesic and anti-nociceptive properties were meticulously dissected. Moreover, the authors also discuss a few of the pharmacological aspect of C. nutans leaves extracts against anti-hyperlipidemia, vasorelaxation and renoprotective activities, which are seldom available from the previously discussed review papers. From the aspect of toxicological studies, controversial findings have been reported in both in-vitro and in-vivo experiments. Thus, further investigations on their phytochemical compounds and their mode of action showing pharmacological activities are required to fully grasp both traditional usage and their suitability for future drugs development. Data related to therapeutic activity and the constituents of C. nutans leaves were searched by using the search engines Google scholar, PubMed, Scopus and Science Direct, and accepting literature reported between 2010 to present. On the whole, this review paper compiles all the available contemporary data from this subtropical herb on its phytochemistry and pharmacological activities with a view towards garnering further interest in exploring its use in cardiovascular and renal diseases.

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“…Additionally, C. nutans contains sulfur‐containing compounds, which have been reported to possess antioxidant and anti‐inflammatory properties (Khoo et al, 2019). Our group has recently demonstrated the potential nephroprotective effect of C. nutans aqueous extract (1000 μg/mL) against cisplatin‐induced nephrotoxicity in renal proximal tubular cells (NRK‐52E) of rat at 89% cell viability (Mahmod et al, 2020). This has piqued our interest in delving deeper into the nephroprotective potential of C. nutans extracts on human kidney cells (PCS‐400‐010).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Clinacanthus nutans in cisplatin‐induced nephrotoxicity on human kidney cell (PCS‐400‐010) elucidated by an LCMS‐based metabolomics approach

Mahmod,

Ismail,

Normi

et al. 2023

Biomedical Chromatography

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Cisplatin‐induced nephrotoxicity has been widely reported in numerous studies. The objective of this study is to assess the potential nephroprotective effects of Clinacanthus nutans (Burm. f.) Lindau (Acanthaceae) leaf extracts on human kidney cells (PCS‐400‐010) in vitro using an LCMS‐based metabolomics approach. Orthogonal partial least square‐discriminant analysis identified 16 significantly altered metabolites when comparing the control and pre‐treated C. nutans cisplatin‐induced groups. These metabolites were found to be associated with glycerophospholipid, purine, and amino acid metabolism, as well as the glycolysis pathway. Pre‐treatment with C. nutans aqueous extract (125 μg/mL) for 24 h, followed by 48 h of cisplatin induction in PCS‐400‐010 cells, demonstrated a nephroprotective effect, particularly involving the regulation of amino acid metabolism.

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NMR and LCMS analytical platforms exhibited the nephroprotective effect of Clinacanthus nutans in cisplatin-induced nephrotoxicity in the in vitro condition

Cited by 13 publications

References 45 publications

Characterization of α-Glucosidase Inhibitors from Psychotria malayana Jack Leaves Extract Using LC-MS-Based Multivariate Data Analysis and In-Silico Molecular Docking

Characterization of α-Glucosidase Inhibitors from Psychotria malayana Jack Leaves Extract Using LC-MS-Based Multivariate Data Analysis and In-Silico Molecular Docking

A Narrative Review on the Phytochemistry, Pharmacology and Therapeutic Potentials of Clinacanthus nutans (Burm. f.) Lindau Leaves as an Alternative Source of Future Medicine

Protective effect of Clinacanthus nutans in cisplatin‐induced nephrotoxicity on human kidney cell (PCS‐400‐010) elucidated by an LCMS‐based metabolomics approach

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